identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
F22E84601753AF7AFF45B1A23B53FAE5.text	F22E84601753AF7AFF45B1A23B53FAE5.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification, and sequencing</p><p>Methods for DNA extraction and PCR amplification referred to He et al. (2023). The primer pairs used in this study were: ITS: ITS5/ITS4 (White et al. 1990); LSU: LR0R/LR5 (Vilgalys &amp; Hester 1990); SSU: NS1/NS4 (White et al. 1990); and tef1: tef1 -983F/ tef1 -2218R (Rehner &amp; Buckley 2005). The total volume of the PCR reaction system is 25 μl, containing 2 μl of DNA template, 1 μl of each primer, and 21 μl of 1 × Power Taq PCR MasterMix. The PCR conditions for amplifying ITS, LSU, and SSU gene loci are: initial denaturation at 95°C for 5 min, followed by 35 cycles of denaturation at 95°C for 15 s, annealing at 55°C for 15 s, and extension at 72°C for 15 s, and finally extension at 72°C for 10 min. The PCR reaction program of tef1 is initial denaturation at 98°C for 5 min, followed by 35 cycles of denaturation at 98°C for 45 s, annealing at 52°C for 45 s, extension at 72°C for 50 s, and finally extension at 72°C for 10 min. PCR products were detected by 1% agarose gel electrophoresis. The purification and sequencing were completed by Shanghai Sangon Bioengineering Co., Ltd.</p></div>	https://treatment.plazi.org/id/F22E84601753AF7AFF45B1A23B53FAE5	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Sun, Bing-Bing;He, Shu-Cheng;Yu, Feng-Ming;Li, Cui-Jin-Yi;Yang, Yan-Yan;Wang, Jian-Rui;Zhao, Qi	Sun, Bing-Bing, He, Shu-Cheng, Yu, Feng-Ming, Li, Cui-Jin-Yi, Yang, Yan-Yan, Wang, Jian-Rui, Zhao, Qi (2025): A new species of Periconia (Periconiaceae, Pleosporales) from Xizang, China. Phytotaxa 693 (2): 133-145, DOI: 10.11646/phytotaxa.693.2.2, URL: https://doi.org/10.11646/phytotaxa.693.2.2
F22E84601757AF7DFF45B2DE3A84FBF7.text	F22E84601757AF7DFF45B2DE3A84FBF7.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Periconia motuoensis B. B. Sun, S. C. He & Q. Zhao 2025	<div><p>Periconia motuoensis B.B. Sun, S.C. He &amp; Q. Zhao, sp. nov. Fig. 2.</p><p>Index Fungorum number: IF902655; Facesoffungi number: FoF 16560</p><p>Etymology: The specific epithet refers to the locality “Motuo” from where the holotype was collected.</p><p>Saprobic on dead sheaths of Poaceae . Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies numerous on substrate, dark brown to black, hairy. Conidiophores 430−600 μm × 13−21 μm (x̅ = 520 × 16 μm, n = 20), macronematous, mononematous, erect, straight or flexuous, branched at the apex, smooth, thick-walled, solitary, septate, brown to black. Conidiogenous cells polyblastic, integrated, terminal, subglobose, smooth to verruculose, brown. Conidia 21−26 μm × 10−14 μm (x̅ = 24 × 12 μm, n = 30), acrogenous, ellipsoidal to cylindrical, verrucose, with two guttulates when mature, thick- and rough-walled, sometimes septate, pale brown to brown.</p><p>Culture characteristics: Conidia germinated on PDA within 24 hours, and produced one or two germ tubes. After three weeks at 25℃, the colony diameter reached about 8 cm with white edges and a grey center, appearing round, flocculent, and with sparsely hairy edges. The reverse of the colony was grey with white edges.</p><p>Material examined: CHINA, Xizang, Linzhi, Motuo, on dead sheaths of Poaceae, 12 July 2022, coll. Yan-Yan Yang, YYY319 (HKAS 135698, holotype), ex-type culture KUNCC24-17924 ; ibid., YYY319-2 (HKAS 135699, isotype), living culture KUNCC24-17925; ibid, 9 August 2023, coll. Shucheng He, SBB39 (HKAS 134948), living culture KUNCC24-17926; ibid., SBB39A (HKAS 134949), living culture KUNCC24-17927 .</p><p>Notes: Periconia motuoensis clustered with P. imperatae, P. spodiopogonis and P. submersa in the phylogenetic tree with high supports (ML / PP = 77% / 1.00) (Fig. 1). Based on the analyses of molecular data, P. motuoensis differs from P. spodiopogonis by 2.65 % in ITS, 0.12 % in LSU, no difference in SSU, and 1.25 % in tef1, and it differs from P. imperatae by 2.62 % in ITS, 0.13 % in LSU, no difference in SSU, and 1.11 % in tef1; as well as it differs from P. submersa by 3.01 % in ITS, 0.02 % in LSU, no difference in SSU and 1.12 % in tef1. Morphologically, the conidia of P. motuoenis are similar to P. aquatica, P. imperatae, and P. spodiopogonis, all of which are nearly ellipsoidal. However, the conidia of P. motuoensis are significantly larger than the ones of P. imperatae and P. aquatica (21−26 μm × 10−14 μm vs. 8.5−13.5 × 5.5−8.5 μm and 10−12 × 6−7 μm respectively). Compared with P. spodiopogonis, the conidia of P. motuoensis are slightly constricted in the middle, longer (21−26 × 10−14 μm vs. 14.5−24.5 × 9.5−15.5 μm), and have two obvious guttulates when mature. In addition, five species, viz., P. chandoliensis, P. guangdongensis, P. notabilis, P. palmivora, and P. pergularigena, lack molecular information, and they can be easily separated from P. motuoenis because of their spherical or nearly spherical conidia.</p></div>	https://treatment.plazi.org/id/F22E84601757AF7DFF45B2DE3A84FBF7	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Sun, Bing-Bing;He, Shu-Cheng;Yu, Feng-Ming;Li, Cui-Jin-Yi;Yang, Yan-Yan;Wang, Jian-Rui;Zhao, Qi	Sun, Bing-Bing, He, Shu-Cheng, Yu, Feng-Ming, Li, Cui-Jin-Yi, Yang, Yan-Yan, Wang, Jian-Rui, Zhao, Qi (2025): A new species of Periconia (Periconiaceae, Pleosporales) from Xizang, China. Phytotaxa 693 (2): 133-145, DOI: 10.11646/phytotaxa.693.2.2, URL: https://doi.org/10.11646/phytotaxa.693.2.2
