taxonID	type	description	language	source
D14C8E3AFFE9FFB2FF3EF8DF32D9551A.taxon	description	A nuclear region of partial 18 S to 28 S rRNA including full ITS 1 + 5.8 S + ITS 2 region (3,760 bp) was amplified with polymerase chain reaction (PCR) with a primer set (forward: 18 F 01, 5 ′ - CACCTGGTTGATCCTGCCAGTAG- 3 ′; reverse: 28 R 1318, 5 ’ - TCGGCAGGTGAGTTGTTACACAC- 3 ′). The 20 μL PCR reaction mixture was composed of 12.8 μL sterile distilled water, 2 μL 10 × Ex PCR buffer (Takara Shuzo, Kyoto, Japan), 2 μL dNTP mix (4 mM each), 1 μL of each primer (10 pmol), 0.2 μL Ex Taq polymerase (2.5 U), and 1 μL of template. The PCR performing conditions were as follows: 94 ℃ for 3 min; 40 cycles of 94 ℃ for 30 sec, 61 ℃ for 40 sec, and 72 ℃ for 2 min; and 72 ℃ for 10 min. The PCR product was confirmed with 1.5 % agarose gel (Condalab, Madrid, Spain) stained with Midori Green under ultraviolet light. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen GmbH, Hilden, Germany). DNA was sequenced by the sanger sequencing method with the ABI 3730 DNA sequencer using the same primer for PCR (Applied Biosystems, Foster City, CA). The complete sequence of the amplified DNA was obtained through primer walking. The obtained DNA fragments were assembled and edited with Sequencher 5.1 (Gene Codes Corporation, Ann Arbor, MI).	en	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
D14C8E3AFFEBFFB3FF3EFB7F32D7544A.taxon	description	Description: Cells are green to light yellowish-green, single, planktonic, and spherical. Cell wall is sculptured in SEM observation. They are 1.5 to 3.5 μm in diameter, with an average of 2.75 μm. They lack a mucilaginous envelope and stalks. They do not form colonies, except during propagation two or four autospores after sporulation remain attached for a short time. The chloroplast is single, parietal, and cup-shaped without pyrenoids. This species is distinguished from other Mychonastes species by the nucleotide composition of the rRNA ITS 2 sequence and its secondary structure.	en	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
D14C8E3AFFEBFFB3FF3EFB7F32D7544A.taxon	etymology	Etymology: The name of the country (Korea) where the species was discovered was reflected.	en	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
D14C8E3AFFEBFFB3FF3EFB7F32D7544A.taxon	materials_examined	Holotype: A permanent microscopic slide, NIBRCL 0000117313 in the Herbarium at the National Institute of Biological Resources, from cultured strain SMU-GA 001. Type locality: Yun-gang River, Toegang-ri, Sabeolguk-myeon, Sangju-si, Gyeongsangbuk-do, Korea (36 ° 31 ' 36.3 " N, 128 ° 15 ' 50.5 " E).	en	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
D14C8E3AFFEBFFB3FF3EFB7F32D7544A.taxon	distribution	Distribution: To date, Mychonastes koreanus has only been observed in this type of locality. At the time of collection, the pH was 8.0, and the water temperature was 17.1 ° C. Gene sequences: partial 18 S rRNA, ITS 1, 5.8 S, ITS 2, and partial 28 S rRNA gene sequences with GenBank accession number OR 914224.	en	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
