identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
D14C8E3AFFE9FFB2FF3EF8DF32D9551A.text	D14C8E3AFFE9FFB2FF3EF8DF32D9551A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction	<div><p>DNA extraction, amplification, and assembly</p><p>The strain was harvested with 0.2 μm pore sized membrane filter (Millipore, Billerica, MA) and treated with 800 μL cetyltrimethylammonium bromide (CTAB) buffer (100 mM tris-HCl pH 8.0, 100mM Na2EDTA, 100 mM sodium phosphate pH 8.0, 1.5M NaCl, 1% CTAB). The sample was stored at -20°C until further experiment. Total genomic DNA was extracted following the CTAB method (Richards et al. 1994).</p><p>A nuclear region of partial 18S to 28S rRNA including full ITS1 + 5.8S + ITS2 region (3,760 bp) was amplified with polymerase chain reaction (PCR) with a primer set (forward: 18F01, 5′-CACCTGGTTGATCCTGCCAGTAG-3′; reverse: 28 R1318, 5 ’-TCGGCAGGTGAGTTGTTACACAC-3′). The 20 μL PCR reaction mixture was composed of 12.8 μL sterile distilled water, 2 μL 10× Ex PCR buffer ( Takara Shuzo, Kyoto, Japan), 2 μL dNTP mix (4mM each), 1 μL of each primer (10 pmol), 0.2 μL Ex Taq polymerase (2.5U), and 1 μL of template. The PCR performing conditions were as follows: 94℃ for 3 min ; 40 cycles of 94℃ for 30 sec, 61℃ for 40 sec, and 72℃ for 2 min; and 72℃ for 10 min. The PCR product was confirmed with 1.5% agarose gel (Condalab, Madrid, Spain) stained with Midori Green under ultraviolet light. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen GmbH, Hilden, Germany) .</p><p>DNA was sequenced by the sanger sequencing method with the ABI3730 DNA sequencer using the same primer for PCR (Applied Biosystems, Foster City, CA). The complete sequence of the amplified DNA was obtained through primer walking. The obtained DNA fragments were assembled and edited with Sequencher 5.1 (Gene Codes Corporation, Ann Arbor, MI).</p></div>	https://treatment.plazi.org/id/D14C8E3AFFE9FFB2FF3EF8DF32D9551A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Lee, Ha-Eun;Kim, Taehee;Ki, Jang-Seu	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
D14C8E3AFFEBFFB3FF3EFB7F32D7544A.text	D14C8E3AFFEBFFB3FF3EFB7F32D7544A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Mychonastes koreanus H. Lee, T. Kim, & J. Ki 2025	<div><p>Mychonastes koreanus H. Lee, T. Kim, &amp; J. Ki sp. nov. (Fig. 2A–E)</p><p>Description: Cells are green to light yellowish-green, single, planktonic, and spherical. Cell wall is sculptured in SEM observation. They are 1.5 to 3.5 μm in diameter, with an average of 2.75 μm. They lack a mucilaginous envelope and stalks. They do not form colonies, except during propagation two or four autospores after sporulation remain attached for a short time. The chloroplast is single, parietal, and cup-shaped without pyrenoids. This species is distinguished from other Mychonastes species by the nucleotide composition of the rRNA ITS2 sequence and its secondary structure.</p><p>Etymology: The name of the country (Korea) where the species was discovered was reflected.</p><p>Holotype: A permanent microscopic slide, NIBRCL0000117313 in the Herbarium at the National Institute of Biological Resources, from cultured strain SMU-GA001.</p><p>Type locality: Yun-gang River, Toegang-ri, Sabeolguk-myeon, Sangju-si, Gyeongsangbuk-do, Korea (36°31'36.3"N, 128°15'50.5"E).</p><p>Distribution: To date, Mychonastes koreanus has only been observed in this type of locality. At the time of collection, the pH was 8.0, and the water temperature was 17.1 °C.</p><p>Gene sequences: partial 18S rRNA, ITS1, 5.8S, ITS2, and partial 28S rRNA gene sequences with GenBank accession number OR914224.</p></div>	https://treatment.plazi.org/id/D14C8E3AFFEBFFB3FF3EFB7F32D7544A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Lee, Ha-Eun;Kim, Taehee;Ki, Jang-Seu	Lee, Ha-Eun, Kim, Taehee, Ki, Jang-Seu (2025): Description of Mychonastes koreanus sp. nov. (Sphaeropleales, Chlorophyceae) isolated from the Yun River, Republic of Korea. Phytotaxa 693 (4): 255-269, DOI: 10.11646/phytotaxa.693.4.1, URL: https://doi.org/10.11646/phytotaxa.693.4.1
