taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
C549878B753DFFC6FC9B1313A2E787F3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877618/files/figure.png	https://doi.org/10.5281/zenodo.16877618	Fig. 1. Phylogenomic placement of the novel taxa within the genus Torulaspora. The phylogeny is based on a concatenated alignment of 3766 single-copy orthologs from 77 Torulaspora genomes and was rooted with Zygosaccharomyces bailii. The JTT+F+I+G4 model of sequence evolution and the maximum-likelihood method were employed. Black bars highlight the novel species, and white bars represent the known ones. Nodes with black dots represent ≥ 95 % bootstrap support. The scale bar corresponds to the estimated number of substitutions per site. For the proposed novel species, numerals in black circles depict ANI values to their closest relatives.	Fig. 1. Phylogenomic placement of the novel taxa within the genus Torulaspora. The phylogeny is based on a concatenated alignment of 3766 single-copy orthologs from 77 Torulaspora genomes and was rooted with Zygosaccharomyces bailii. The JTT+F+I+G4 model of sequence evolution and the maximum-likelihood method were employed. Black bars highlight the novel species, and white bars represent the known ones. Nodes with black dots represent ≥ 95 % bootstrap support. The scale bar corresponds to the estimated number of substitutions per site. For the proposed novel species, numerals in black circles depict ANI values to their closest relatives.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B753DFFC6FC9B1313A2E787F3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877622/files/figure.png	https://doi.org/10.5281/zenodo.16877622	Fig. 2. Phenotype and gene content variation related to the utilization of selected carbohydrates across the genus Torulaspora. Squares and circles correspond to phenotypes and genotypes, respectively. A filled shape indicates a positive result (growth, G; fermentation, F; or gene presence), an empty shape indicates a negative result, and an absent shape indicates a lack of testing for that trait. Genes are designated using standard nomenclatures and capital letters corresponding to their encoded activities (isomal, isomaltase; mal, maltase; unk, unknown, encoding either for a maltase or isomaltase). Fermentation and assimilation results were obtained in this work or were taken from the literature as indicated in Table S3.	Fig. 2. Phenotype and gene content variation related to the utilization of selected carbohydrates across the genus Torulaspora. Squares and circles correspond to phenotypes and genotypes, respectively. A filled shape indicates a positive result (growth, G; fermentation, F; or gene presence), an empty shape indicates a negative result, and an absent shape indicates a lack of testing for that trait. Genes are designated using standard nomenclatures and capital letters corresponding to their encoded activities (isomal, isomaltase; mal, maltase; unk, unknown, encoding either for a maltase or isomaltase). Fermentation and assimilation results were obtained in this work or were taken from the literature as indicated in Table S3.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B753DFFC6FC9B1313A2E787F3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877624/files/figure.png	https://doi.org/10.5281/zenodo.16877624	Fig. 3. Phylogeny of all the species of the genus Torulaspora based on the complete ITS region. A. Phylogenetic tree constructed with the maximum-likelihood method and the TPM2u model of sequence evolution, chosen according to the Bayesian information criterion (BIC), and rooted with Zygotorulaspora mrakii. The final dataset had a total of 813 positions. Names in bold indicate the novel species. The scale bar represents the estimated number of substitutions per site. Percentage bootstrap values of 1000 replicates equal or higher that 95 % are depicted as black circles at tree nodes. B. For the most closely related species, the number of nucleotide substitutions in the ITS alignment is given. C. For the species pairs exhibiting the lowest number of nucleotide substitutions the location and nature of those substitutions is given (position 1 is the first position after the primer).	Fig. 3. Phylogeny of all the species of the genus Torulaspora based on the complete ITS region. A. Phylogenetic tree constructed with the maximum-likelihood method and the TPM2u model of sequence evolution, chosen according to the Bayesian information criterion (BIC), and rooted with Zygotorulaspora mrakii. The final dataset had a total of 813 positions. Names in bold indicate the novel species. The scale bar represents the estimated number of substitutions per site. Percentage bootstrap values of 1000 replicates equal or higher that 95 % are depicted as black circles at tree nodes. B. For the most closely related species, the number of nucleotide substitutions in the ITS alignment is given. C. For the species pairs exhibiting the lowest number of nucleotide substitutions the location and nature of those substitutions is given (position 1 is the first position after the primer).	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B753DFFC6FC9B1313A2E787F3.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7539FFCAFC9B1031A1358190.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7535FFCAFF251471A5148453.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7535FFCAFC9B1031A4A28350.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7535FFCBFC9B1731A0A18610.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7534FFCBFFD512F1A5408430.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7534FFCBFFD512F1A5408430.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877622/files/figure.png	https://doi.org/10.5281/zenodo.16877622	Fig. 2. Phenotype and gene content variation related to the utilization of selected carbohydrates across the genus Torulaspora. Squares and circles correspond to phenotypes and genotypes, respectively. A filled shape indicates a positive result (growth, G; fermentation, F; or gene presence), an empty shape indicates a negative result, and an absent shape indicates a lack of testing for that trait. Genes are designated using standard nomenclatures and capital letters corresponding to their encoded activities (isomal, isomaltase; mal, maltase; unk, unknown, encoding either for a maltase or isomaltase). Fermentation and assimilation results were obtained in this work or were taken from the literature as indicated in Table S3.	Fig. 2. Phenotype and gene content variation related to the utilization of selected carbohydrates across the genus Torulaspora. Squares and circles correspond to phenotypes and genotypes, respectively. A filled shape indicates a positive result (growth, G; fermentation, F; or gene presence), an empty shape indicates a negative result, and an absent shape indicates a lack of testing for that trait. Genes are designated using standard nomenclatures and capital letters corresponding to their encoded activities (isomal, isomaltase; mal, maltase; unk, unknown, encoding either for a maltase or isomaltase). Fermentation and assimilation results were obtained in this work or were taken from the literature as indicated in Table S3.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7534FFCBFFD512F1A5408430.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877624/files/figure.png	https://doi.org/10.5281/zenodo.16877624	Fig. 3. Phylogeny of all the species of the genus Torulaspora based on the complete ITS region. A. Phylogenetic tree constructed with the maximum-likelihood method and the TPM2u model of sequence evolution, chosen according to the Bayesian information criterion (BIC), and rooted with Zygotorulaspora mrakii. The final dataset had a total of 813 positions. Names in bold indicate the novel species. The scale bar represents the estimated number of substitutions per site. Percentage bootstrap values of 1000 replicates equal or higher that 95 % are depicted as black circles at tree nodes. B. For the most closely related species, the number of nucleotide substitutions in the ITS alignment is given. C. For the species pairs exhibiting the lowest number of nucleotide substitutions the location and nature of those substitutions is given (position 1 is the first position after the primer).	Fig. 3. Phylogeny of all the species of the genus Torulaspora based on the complete ITS region. A. Phylogenetic tree constructed with the maximum-likelihood method and the TPM2u model of sequence evolution, chosen according to the Bayesian information criterion (BIC), and rooted with Zygotorulaspora mrakii. The final dataset had a total of 813 positions. Names in bold indicate the novel species. The scale bar represents the estimated number of substitutions per site. Percentage bootstrap values of 1000 replicates equal or higher that 95 % are depicted as black circles at tree nodes. B. For the most closely related species, the number of nucleotide substitutions in the ITS alignment is given. C. For the species pairs exhibiting the lowest number of nucleotide substitutions the location and nature of those substitutions is given (position 1 is the first position after the primer).	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7534FFCBFC941011A53A8330.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7534FFCCFC941711A4758770.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7533FFCDFC9B1351A2618050.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7532FFCDFFD51431A3138490.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7532FFCDFC941371A5618330.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7532FFCEFC941711A1B18630.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
C549878B7531FFCEFF251211A1A582D0.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16877626/files/figure.png	https://doi.org/10.5281/zenodo.16877626	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	Fig. 4. Vegetative and sexual structures of the novel Torulaspora species. For each species, the first micrograph corresponds to budding yeast cells on YM agar after 3–5 d at 25 °C. Micrographs of asci, ascospores, or other structures were obtained from cultures grown on acetate agar for 1–3 wk at 17–20 °C. A–C. Yeast cells of T. asahi PYCC 8100T (A), cells with tapered protuberances (B), and ascus with ascospore (C). D. Yeast cells of T. cukson f.a. PYCC 9174T. E–G. Yeast cells of T. floroides UFMG-CM-Y6992T (E), peculiar arrangement of bud cells resembling a flower (F), and ascus with two ascospores (G). H–J. Yeast cells of T. gathmanii PYCC 9889T (H) and asci with ascospores (I, J). K, L. Yeast cells of T. incommunis CBS 5080T (K) and ascus with two ascospores (L). M–O. Yeast cells of T. kanakia PYCC 8417T (M), long protuberances, and asci with ascospores (N, O). P–R. Yeast cells of T. mapucheana PYCC 9893T (P), cell with protuberance (Q), and ascus with two ascospores (R). S, T. Yeast cells of T. markjohnstonii PYCC 9891T (S), a cell with a tapered protuberance, and an ascus containing two ascospores (T). U–W. Yeast cells of T. nezacadeziae PYCC 8102T (U), protuberances, and asci with ascospores (V, W). X–Z. Yeast cells of T. obscura f.a. PYCC 8933T (X). Yeast cells of T. pahokee CBS 11100T (Y) and asci with ascospores (Z). AA. Yeast cells of T. ventriculi f.a. PYCC 2995T. Scale bars = 10 µm.	2025-05-23	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.		Zenodo	biologists	Silva, M. R.;Paraíso, F.;Al-Oboudi, J.;Abegg, M.;Aires, A.;Barros, K. O.;Brito, P. H.;Jarzyna, M.;Sylvester, K.;Langdon, Q. K.;Opulente, D. A.;Carriconde, F.;Fell, J. W.;Hofmann, T. A.;Lachance, M. - A.;Legras, J. - L.;Libkind, D.;Pontes, A.;Gonçalves, P.;Rosa, C. A.;Groenewald, M.;Hittinger, C. T.;Sampaio, J. P.			
