taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707834/files/figure.png	https://doi.org/10.5281/zenodo.16707834	FIGURES 4–6. LM images of Actinocyclus aquae-dulcis (living cells) from Lake Akan.The white arrow indicates a chloroplast; the black arrow marks a nucleus. (4, 5) Valve views. (6) Girdle view. Scale bar = 10 μm.	FIGURES 4–6. LM images of Actinocyclus aquae-dulcis (living cells) from Lake Akan.The white arrow indicates a chloroplast; the black arrow marks a nucleus. (4, 5) Valve views. (6) Girdle view. Scale bar = 10 μm.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707840/files/figure.png	https://doi.org/10.5281/zenodo.16707840	FIGURES 7–9. LM images of Actinocyclus aquae-dulcis holotype from Lake Akan. Scale bar = 10 μm.	FIGURES 7–9. LM images of Actinocyclus aquae-dulcis holotype from Lake Akan. Scale bar = 10 μm.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707844/files/figure.png	https://doi.org/10.5281/zenodo.16707844	FIGURES 10–15. LM images of Actinocyclus aquae-dulcis from Lake Akan and variations in cell size. The white arrows each indicate a rimoportula; the black arrows each mark a pseudonodule. Scale bar = 10 μm.	FIGURES 10–15. LM images of Actinocyclus aquae-dulcis from Lake Akan and variations in cell size. The white arrows each indicate a rimoportula; the black arrows each mark a pseudonodule. Scale bar = 10 μm.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707848/files/figure.png	https://doi.org/10.5281/zenodo.16707848	FIGURES 16–24. SEM images of Actinocyclus aquae-dulcis from Lake Akan. (16) External valve view. (17, 18) Internal valve views. White arrows denote rimoportulae. (19–21) External girdle views. (22) Pseudonodule in external valve view; black arrows mark pseudonodules. (23) Loculate areolae in external valve view. (24) Cross section of the areolae. Scale bar = 10 μm in (16–21), and 1 μm in (22–24).	FIGURES 16–24. SEM images of Actinocyclus aquae-dulcis from Lake Akan. (16) External valve view. (17, 18) Internal valve views. White arrows denote rimoportulae. (19–21) External girdle views. (22) Pseudonodule in external valve view; black arrows mark pseudonodules. (23) Loculate areolae in external valve view. (24) Cross section of the areolae. Scale bar = 10 μm in (16–21), and 1 μm in (22–24).	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707852/files/figure.png	https://doi.org/10.5281/zenodo.16707852	FIGURES 25–31. SEM images of Actinocyclus aquae-dulcis from Lake Akan, internal valve and the rimoportulae. (25–28) Complete rimoportulae. (27) Complete rimoportula and the split ring spacing (open band, on the left side). (29–31) Broken rimoportulae. White arrow denotes a rimoportula. Scale bar = 1 μm in (25–27) and 5 μm in (28–31).	FIGURES 25–31. SEM images of Actinocyclus aquae-dulcis from Lake Akan, internal valve and the rimoportulae. (25–28) Complete rimoportulae. (27) Complete rimoportula and the split ring spacing (open band, on the left side). (29–31) Broken rimoportulae. White arrow denotes a rimoportula. Scale bar = 1 μm in (25–27) and 5 μm in (28–31).	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707856/files/figure.png	https://doi.org/10.5281/zenodo.16707856	FIGURES 32–35. LM images of Actinocyclus aquae-dulcis from the Yoneshiro River mouth. The white arrow indicates a rimoportula; the black arrow marks a pseudonodule. (32) Valve view of living cell and chloroplasts. (33) Girdle view of living cell and chloroplasts. Scale bar = 20 μm.	FIGURES 32–35. LM images of Actinocyclus aquae-dulcis from the Yoneshiro River mouth. The white arrow indicates a rimoportula; the black arrow marks a pseudonodule. (32) Valve view of living cell and chloroplasts. (33) Girdle view of living cell and chloroplasts. Scale bar = 20 μm.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707830/files/figure.png	https://doi.org/10.5281/zenodo.16707830	FIGURES 1–3. Study area. (1) Maps showing the study area and areas where Actinocyclus aquae-dulcis has been reported. The sampling locality of Actinocyclus normanii (Lake Kasumigaura) is also shown. (2) Sampling locality of A. aquae-dulcis in Lake Akan. (3) Sampling locality of A. aquae-dulcis in the Yoneshiro River mouth area.	FIGURES 1–3. Study area. (1) Maps showing the study area and areas where Actinocyclus aquae-dulcis has been reported. The sampling locality of Actinocyclus normanii (Lake Kasumigaura) is also shown. (2) Sampling locality of A. aquae-dulcis in Lake Akan. (3) Sampling locality of A. aquae-dulcis in the Yoneshiro River mouth area.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707860/files/figure.png	https://doi.org/10.5281/zenodo.16707860	FIGURE 36. Relationship between valve diameter and number of rimoportulae in Actinocyclus aquae-dulcis from Lake Akan and the Yoneshiro River mouth. White circles show the Yoneshiro River mouth sample and black diamonds show the Lake Akan sample.	FIGURE 36. Relationship between valve diameter and number of rimoportulae in Actinocyclus aquae-dulcis from Lake Akan and the Yoneshiro River mouth. White circles show the Yoneshiro River mouth sample and black diamonds show the Lake Akan sample.	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
B2232643595A0574FF402B21FE1AC0AC.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/16707864/files/figure.png	https://doi.org/10.5281/zenodo.16707864	FIGURE 37. The phylogenetic tree of Actinocyclus aquae-dulcis and related strains determined using the Maximum Likelihood (ML) method with rbcL (GTR+G+I) gene. Accession numbers, taxonomic names, and strain names follow localities for Japanese strains, and NJ/ML bootstrap support values are represented on branches (only values above 70 are shown).	FIGURE 37. The phylogenetic tree of Actinocyclus aquae-dulcis and related strains determined using the Maximum Likelihood (ML) method with rbcL (GTR+G+I) gene. Accession numbers, taxonomic names, and strain names follow localities for Japanese strains, and NJ/ML bootstrap support values are represented on branches (only values above 70 are shown).	2025-02-13	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko		Zenodo	biologists	Chiba, Takashi;Osada, Ryosuke;Tuji, Akihiro;Idei, Masahiko			
