identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
987F3839FF9C9000FF2AFB80655CDDA5.text	987F3839FF9C9000FF2AFB80655CDDA5.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna	<div><p>DNA Extraction, PCR Amplification, and Sequencing</p><p>Fungal genomic DNA was extracted from mycelia using the Trelief™ Plant Genomic DNA Kit (Beijing Qingke Biotech) in accordance with the manufacturer’s protocol. Based on a comprehensive review of the pertinent literature, suitable DNA barcodes and corresponding primer sequences were selected, and PCR reactions were carried out. Four DNA barcodes were utilized, and the primers employed included ITS9mun and ITS4_KYo1 (Toju et al. 2012) for the internal transcribed spacer (ITS), LR0R (Vilgalys &amp; Hester 1990) and LR5 (Cubeta et al. 1991) for the large subunit ribosomal RNA (LSU), fRPB2-7cR and fRPB2-5F (Voglmayr et al. 2016) for the second largest subunit of RNA polymerase II (rpb2), and EF1-728F and EF1-986R (Rehner et al. 2005) for translation elongation factor 1-alpha (tef1-α). The final PCR reaction system was set at 30 μL, consisting of 15 μL PCR Master Mix (CoWin Biosciences, Taizhou, China), 11 μL ddH 2 O, 1 μL each of forward and reverse primers (10 μM), and 2 μL of DNA template. The thermal cycling conditions for ITS, LSU, tef1-α, and rpb2 were set as follows: an initial denaturation occurred at 94 °C for 5 minutes, followed by 35 cycles. For ITS, LSU, and tef1-α, each cycle consisted of denaturation at 94 °C for 45 seconds, annealing at 56 °C for 50 seconds, and extension at 72 °C for 1 minute. For rpb2, each cycle included denaturation at 95 °C for 60 seconds, annealing at 56 °C for 120 seconds, and extension at 72 °C for 90 seconds. A final extension at 72 °C for 10 minutes was performed to ensure complete amplification. The PCR products were analysed using 1% agarose gel electrophoresis, followed by Sanger sequencing conducted by Sangon Biotech Co., Ltd. After obtaining the raw sequencing data, the SeqMan v.7.1.0 software was employed to evaluate the chromatogram files, check sequencing quality, trim the unstable terminal sequences, and compile the bidirectional sequencing results.</p></div>	https://treatment.plazi.org/id/987F3839FF9C9000FF2AFB80655CDDA5	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Ding, Peng-Cheng;Madhushan, Asanka;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Ding, Peng-Cheng, Madhushan, Asanka, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): A New Species of Dendrostoma (Erythrogloeaceae, Diaporthales) Identified by Combined Morphological and Phylogenetic Studies. Phytotaxa 714 (2): 181-190, DOI: 10.11646/phytotaxa.714.2.6, URL: https://doi.org/10.11646/phytotaxa.714.2.6
987F3839FF9E9005FF2AF9F462F7D806.text	987F3839FF9E9005FF2AF9F462F7D806.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dendrostoma multiguttulum P. C. Ding, Madhushan & Maharachch 2025	<div><p>Dendrostoma multiguttulum P.C. Ding, Madhushan &amp; Maharachch, sp. nov. (FIGURE 2)</p><p>MycoBank: 859477</p><p>Etymology – The name reflects the presence of numerous (multi) small drop-like spots (gutulum) on the conidia.</p><p>Asexual morph: Conidiomata pycnidial, conical, solitary, with a single neck, erumpent through the host bark, locule irregularly-shaped, 110–180 high × 370–410 μm diam (= 150.22 × 397.8 μm, n = 30). Conidiomata wall several layers of light yellow irregular tissues 16–32 μm (= 24.73 μm, n = 30. Conidiophores reduced to conidiogenous cells. Conidiogenous cells lining the inner walls of the cavity, hyaline, smooth, subcylindrical to ampulliform, 9–28.5 × 1–2.5 μm (= 18.2 × 1.68 μm, n = 30). Conidia hyaline, aseptate, smooth, multiguttulate, thin-walled, ellipsoid to fusoid 8.9–10.5 × 1.2–3.9 μm (= 8.36 × 2.71 μm, l/w=3, n = 50). Sexual morph: Not observed.</p><p>Colony characteristics: Conidia germinate on PDA at 24 °C within 12 hours, and colonies can reach a diameter of 42 mm after 20 days at the same temperature. Surface is effused, sparse, circular, and white on the surface, transitioning from light brown to black, and white in reverse.</p><p>Materials examined: CHINA, Sichuan Province, Mianyang City, Jiangyou County, WuDu Town, 104˚46'16''N, 31˚54′48′′E, 658 m, 20 November 2024, P.C. Ding (HKAS148723, holotype), ex type culture CGMCC3.28975.</p><p>Notes: In the multi-gene phylogeny, our collection forms an independent branch close to D. rizhaoense and D. castanopsidis with 100% ML bootstrap support and 1.00 BYPP values. The nucleotide sequences of strain D. multiguttulum show a 99% similarity to D. rizhaoense CFCC 57559 for ITS (468/472 bp, no gaps), a 99% similarity for rpb2 (839/842 bp, no gaps), and a 96% similarity for tef1-α (281/293 bp, with 3 gaps). Additionally, they show a 96% similarity to D. castanopsidis CGMCC 3.25676 for ITS (515/538 bp, with 8 gaps), a 99% similarity for LSU (784/786 bp, no gaps), a 96% similarity for rpb2 (869/904 bp, no gaps), and a 96% similarity for tef1-α (281/293 bp, with 3 gaps). Morphologically, our isolate D. multiguttulum shows significant differences from D. rizhaoense (Jiang et al. 2024) and D. castanopsidis (Mu et al. 2024) . The conidiogenous cells of D. multiguttulum are more slender (9–28.5 × 1–2.5 μm) than those of D. rizhaoense (8–27.5 × 3–5.5 μm) and are longer than those of D. castanopsidis (5–12.0 × 1–2 μm). The conidia of D. multiguttulum are more regularly shaped, ranging from ellipsoid to fusoid, whereas those of D. rizhaoense are more variable within the same range, and those of D. castanopsidis are cylindrical to clavate. Additionally, D. multiguttulum has longer, more slender conidia (8.9–10.5 × 1.2–3.9 μm) with more numerous small drop-like spots compared to D. rizhaoense (6.4–8.8 × 2.7–3.8 μm) and D. castanopsidis, which has shorter biguttulate conidia (4.0–6.0 × 1.0–2.0 μm). The differences in both molecular phylogeny and morphological characteristics support the distinction of this taxon. Therefore, based on combined phylogenetic and morphological evidence, this specimen is described here as a new species, D multiguttulum .</p></div>	https://treatment.plazi.org/id/987F3839FF9E9005FF2AF9F462F7D806	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Ding, Peng-Cheng;Madhushan, Asanka;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Ding, Peng-Cheng, Madhushan, Asanka, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): A New Species of Dendrostoma (Erythrogloeaceae, Diaporthales) Identified by Combined Morphological and Phylogenetic Studies. Phytotaxa 714 (2): 181-190, DOI: 10.11646/phytotaxa.714.2.6, URL: https://doi.org/10.11646/phytotaxa.714.2.6
