taxonID	type	description	language	source
6B2F417EFF86FFCAFC84FBD0FEC9FAD5.taxon	description	A previous study have reported the identification of seven UGT 88 F in apple tree genome (Zhou et al., 2017). This result was re-examined by an exhaustive identification of UGT 88 F subfamily members using the updated and improved Malus x domestica genome assembly (Daccord et al., 2017). Based on predicted protein sequence identity to UGT 88 F 1 (Jugdé et al., 2008; threshold of 60 % total protein identity), only five locus tags were obtained instead of seven: MD 15 D 1407600, MD 15 G 1407300, MD 08 G 1219100, MD 08 G 1219000 and MD 08 G 1218900. Among them, the first two correspond respectively to the already reported UGT 88 F 1 (Jugdé et al., 2008) and UGT 88 F 4 (Gosch et al., 2010 a), whereas the last three, which were previously identified by Zhou et al. (2017) as MDP 0000461555, MDP 0000288715 and MDP 0000170162, are uncharacterized members that we have been named UGT 88 F 6 (KC 895981), UGT 88 F 7 (KC 895982) and UGT 88 F 8 (KC 895983) respectively. On the two additional sequences found by Zhou et al. (2017), MDP 0000361449 is a partial sequence corresponding to UGT 88 F 1 which probably arise from incomplete reconstruction of the first version of apple tree genome (Velasco et al., 2010), whereas MDP 0000318101 have a protein identity lower than 60 % with other UGT 88 Fs. Finally, the previously cloned UGT 88 F 5 were not found in either old or new genome versions and seems to be an allelic sequence of UGT 88 F 4 (Gosch et al., 2010 a). The new genome assembly prompt us to investigate the genomic context of the UGT 8 8 F members. Unlike most UGTs, these five gene models do not possess any introns. This unique gene structure, their similar lengths (1446 bp ± 3 to 6 bp) and their high sequence identity between 79,9 and 91,9 % at the protein level (Fig. 2 C), support a common origin. UGT 8 8 F genes are organized into two genomic clusters on chromosome 8 and 15 (Fig. 2 A). UGT 88 F 1 is located closer to UGT 88 F 4 on chromosome 15 and both genes share the same orientation. An additional truncated sequence corresponding to an UGT pseudogene is found nearby UGT 88 F 1. On chromosome 8, UGT 88 F 6, UGT 88 F 7 and UGT 88 F 8 form a physical cluster sharing again the same orientation. Interestingly, UGT organization and genomic environment is conserved between these two clusters (Fig. 2 A). In fact, it has been previously proposed that chromosome 8 originates from a truncated copy of chromosome 15 as a result of the global apple tree genome duplication event (Daccord et al., 2017). However, slight differences between the two clusters can be noticed, indicating modifications (insertion / deletion) after chromosomal duplication event including, among others, partial loss of one UGT sequence on chromosome 15 leading to a pseudogene. Finally, DNA phylogeny analysis revealed a higher identity of UGT within clusters than between clusters (Fig. 2 B). Taking together, these results preclude us from determine whether gene duplication within clusters occurred prior or after the global genome duplication event.	en	Elejalde-Palmett, Carolina, Billet, Kévin, Lanoue, Arnaud, Craene, Johan-Owen De, Glévarec, Gaëlle, Pichon, Olivier, Clastre, Marc, Courdavault, Vincent, St-Pierre, Benoit, Giglioli-Guivarc'h, Nathalie, Bernonville, Thomas Dugé de, Besseau, Sébastien (2019): Genome-wide identification and biochemical characterization of the UGT 88 F subfamily in Malus x domestica Borkh. Phytochemistry 157: 135-144, DOI: 10.1016/j.phytochem.2018.10.019, URL: http://dx.doi.org/10.1016/j.phytochem.2018.10.019
