identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
0820A223DC23FF889A17FE08FE5DFC3A.text	0820A223DC23FF889A17FE08FE5DFC3A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna	<div><p>Taxon and DNA regions sampling</p><p>To analyse the phylogenetic position of the putative new species, we used a total of 110 taxa representing 13 genera of the subfamily Cercidoideae . Of these, ten species of the genus Phanera that are mostly not available in GenBank (http://www. ncbi.nlm.nih.gov/genbank/), including a putative new species and the type species of the genus ( P. coccinea), were newly sequenced. Nuclear ribosomal ITS sequences and the trnL intron and trnL -F spacer (hereafter trnLF region) of Cercidoideae were downloaded from GenBank (http://www.ncbi.nlm.nih. gov/genbank/). Voucher information and GenBank accession numbers are provided in the Appendix. Species of the genus Cercis were chosen as outgroup, thereby following previous studies (Lai et al. 1997, Bruneau et al. 2001, 2008, Hao et al. 2003, Sinou et al. 2009, 2020).</p></div>	https://treatment.plazi.org/id/0820A223DC23FF889A17FE08FE5DFC3A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Mattapha, S.;Suddee, S.;Duangjai, S.;Kiewbang, W.	Mattapha, S., Suddee, S., Duangjai, S., Kiewbang, W. (2022): Phanera mekongensis (Fabaceae: Cercidoideae), a new species from Thailand as supported by morphological and molecular evidence. Blumea 67 (2): 113-122, DOI: 10.3767/blumea.2022.67.02.04, URL: https://doi.org/10.3767/blumea.2022.67.02.04
0820A223DC23FF889A17FC6AFDFAF803.text	0820A223DC23FF889A17FC6AFDFAF803.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification and sequencing</p><p>Leaf samples of the putatively new species and P. kockiana (Korth.) Benth. were collected from cultivated plants at Kasetsart University, Bangkok, and ten other species of Phanera and two species of Cheniella were collected in the field and preserved in silica gel. Total DNA was extracted following the 2 × cetyltrimethylammonium bromide (CTAB) procedure of Doyle &amp; Doyle (1987) with minor modifications. We washed ground plant material with sorbitol buffer (Tel-zur et al. 1999) followed by centrifugation before incubating with CTAB buffer. The trnLF region of our samples was amplified using the universal primers c and f (Taberlet et al. 1991). PCR was performed in a total reaction mixture of 50 μL containing 25 μL of 2 × DreamTaq Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 21 μL of nuclease-free water, 1 μL of bovine serum albumin (BSA (20 mg /mL), 1 μL of each primer (20 mmol/L) and 1 μL of template DNA . The PCR programme consisted of an initial 3 min pre-melt at 94 °C and 35 cycles of 45 sec denaturation at 94 °C, 45 sec annealing at 50 °C, and a 1 min extension at 65 °C, followed by final extension of 6 min at 65 °C. The inter- nal transcribed spacers (ITS) of nuclear ribosomal DNA were amplified using the 5F (Möller &amp; Cronk 2001) and 4R (White et al. 1990) primers. As above, the PCR was performed in a total reaction mixture of 50 μL, but the volume of nuclease-free water was reduced to 20 μL and 1 μL DMSO was added. The PCR programme was the same as above, except that the extension step was conducted at 72 °C. All of the successfully amplified products were cleaned using FastAP Thermosensitive Alkaline Phosphatase and Exonuclease I (Thermo Fisher Scientific, Waltham, MA, USA). Ultimately, cleaned PCR products were sent to Macrogen (Seoul, Korea) for Sanger sequencing using the same primers as in the initial amplification. Raw sequences were edited and assembled using AutoAssembler v. 1.4.0 (Applied Biosystems 1995). All newly generated sequences have been deposited in GenBank (Appendix).</p></div>	https://treatment.plazi.org/id/0820A223DC23FF889A17FC6AFDFAF803	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Mattapha, S.;Suddee, S.;Duangjai, S.;Kiewbang, W.	Mattapha, S., Suddee, S., Duangjai, S., Kiewbang, W. (2022): Phanera mekongensis (Fabaceae: Cercidoideae), a new species from Thailand as supported by morphological and molecular evidence. Blumea 67 (2): 113-122, DOI: 10.3767/blumea.2022.67.02.04, URL: https://doi.org/10.3767/blumea.2022.67.02.04
0820A223DC26FF8E994EFF67FAE5FC54.text	0820A223DC26FF8E994EFF67FAE5FC54.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Phanera mekongensis Mattapha, Suddee & Duangjai 2022	<div><p>Phanera mekongensis Mattapha, Suddee &amp; Duangjai, sp. nov. — Fig. 3, 4; Map 1</p><p>Similar to Phanera hekouensis (T.Y.Tu &amp; D.X.Zhang) Krishnaraj but differs by having shorter pedicels (8–11 mm in P. mekongensis vs 21–34 mm long in P. hekouensis), oblong floral buds (vs spindle-shaped in P. hekouensis), shorter hypanthium (9–11 mm long in P. mekongensis vs 17–21 mm long in P. hekouensis), length of petal limb 7–8 mm long (vs 12–22 mm long in P. hekouensis), anthers densely silky with hairs on the back (vs glabrous in P. hekouensis), stipe of ovary c. 1 mm long (vs 8–15 mm long in P. hekouensis) and a short style (less than 1 mm in P. mekongensis vs 12–19 mm long in P.hekouensis). — Type: Suddee, Puudjaa, Hemrat &amp; Kiewbang 5390 (holo BKF;iso BK,BKF,K, KKU,QBG), Thailand, Bueng Kan Province,Bung Khla District, Phu Wua Wildlife Sanctuary, cultivated in a medicinal garden, altitude c. 190 m, 5 Nov. 2018, fl. For paratypes see below.</p><p>Etymology. The epithet refers to the Mekong River, which runs close to the Phu Wua Wildlife Sanctuary.</p><p>Liana; tendrils opposite, 4–8 cm long, hairy; young twigs densely whitish tomentose when young, becoming ferruginous at maturity. Leaves simple, bifid, spirally arranged, coriaceous; petioles 30–35 mm long, pubescent; lamina ovate, 6–13.5 by 5.5–12 cm, apically bifid to 1/4–1/3 of lamina length with a narrow sinus, midrib bristly between the lobes, lobes ovate to lanceolate, tip of lobes triangular-acute, margin tomentose, base deeply cordate, with 2 rounded to oblong alveoles at base of veins, 9–11-veined from base, tertiary veins reticulate-scalariform; upper surface sparsely pubescent; lower surface greyish velvety when young, densely brownish tomentose when fully grown; stipules ovate-lanceolate, 6–20 by 3–9 mm, apex acute, outer surface densely tomentose, inner surface glabrescent, caducous. Inflorescences terminal racemes, erect, elongated, slender, lax-flowered, 20 – 26 cm long, with 20 – 35 flowers, axis densely tomentose. Pedicels 8–11 mm long, densely pubescent; bracts caducous, ovate to lanceolate, 14–15 by 3–4 mm, apex acuminate, outer surface pubescent, inner surface tomentose; bracteoles caducous, linear to lanceolate, 8–9 by 2–3 mm, apex acuminate, outer surface pubescent, inner surface glabrous, inserted in lower part below the middle of pedicels. Buds oblong, 7–8 mm long, pubescent, apex acute. Hypanthium tubular, 9–11 mm long, densely pubescent. Calyx 5-lobed, splitting regularly at anthesis; lobes lanceolate, 7–8 by 2–3 mm, gradually separate from the base to completely separate at apex, equal, apex acute, margin pubescent, outer</p><p>cm</p><p>2 surface densely pubescent, inner surface glabrous, persistent in fruit. Petals 5, light yellow to slightly light pink, posterior one relatively larger than lateral petals, spathulate or obovate; limb of posterior petal 10–11 by 8–9 mm, limps of lateral petals 8–9 by c. 3.5 mm, apex rounded, margin undulate, crisped, inner surface glabrous, outer surface densely whitish pubescent along veins, more dense pubescent on claw, otherwise glabrous, claw of posterior petal 5 – 6 mm long, claw of lateral petals 6–7 mm long, flattened, pubescent outside and margins. Fertile stamens 3, included in the flowers; filaments 5–6 mm long; anthers brown, more or less flattened, oblong, c. 2.5 by 2 mm, dehiscing by longitudinal slits, versatile, densely silky on the back. Staminodes 0–2; filaments c. 1 mm long; anthers absent. Floral disc absent. Ovary shortly stipitate, densely pubescent all over, 7–9-ovuled; stipe c. 1 mm long; style very short, less than 1 mm long, glabrous at upper part; stigma peltate, c. 1 mm diam. Fruits oblong, flattened, 20–32 by 2.5–4 cm, dehiscent, woody, glabrous, 6–8-seeded; stipe 2–3 cm long. Seeds flattened, elliptic to rounded, 1.4–2 by 1.2–1.7 cm, hilum crescentic, glabrous, black.</p><p>Distribution — Seeds were originally collected from Phu Ngoen Village, Bueng Kan and subsequently cultivated in Phu Wua Wildlife Sanctuary medicinal garden (Fig. 4). The precise habitat remains untraceable, and additional investigations are necessary to explore its distribution site in the natural habitat.</p><p>Habitat &amp; Ecology — Cultivated in dry evergreen forest, elevation c. 190 m.</p><p>Phenology — Flowering: September–October; fruiting: November–February.</p><p>Vernacular name (in Thai) — Kam lang chang phueak (กำ�ลัง ช้�งเผือก).</p><p>Conservation status — Phanera mekongensis is currently known from a single population with a few individuals cultivated in Phu Wua Wildlife Sanctuary. A wild population in the area has not been found. In addition, we have no more information about the occurrence of the species in the areas of Nam Kading National Bio-Diversity Conservation Area, Lao PDR which is rich in endemic species (Tagane et al. 2018). The species can probably be found in the Lao PDR’s area, opposite the Phu Wua Wildlife Sanctuary. Therefore, future intensive floristic investigations are necessary to explore the complete distribution range of the species. We assess it here as data deficient (DD) according to the IUCN Red List Categories (IUCN 2019), due to inadequacy of the distribution information.</p><p>Additional specimens examined (paratypes). CENTRAL, Bangkok, seeds were taken from Bueng Kan Province, but the exact location is unknown, and later cultivated in the Faculty of Forestry, Kasetsart University, 22 Oct. 2018, fl., Duangjai 2018 s.n. (BKF) . – NORTHEASTERN, Bueng Kan, Bung Khla district, Phu Wua Wildlife Sanctuary, cultivated in Medicinal Garden, alt. 190 m, 10 Oct. 2007, fl., Kaewphoung 01 (BKF) ; ibid., 8 Feb. 2018, fr., Suddee, Mattapha, Hemrat &amp; Kiewbang 5343 (BKF); ibid., 15 Sept. 2018, fl., Suddee, Puudjaa, Hemrat &amp; Kiewbang 5378 (BKF); ibid., 15 Oct. 2019, Suddee, Puudjaa, Tetsana, Thananthaisong, Hemrat, Kiewbang &amp; Ue-aree 5568 (BKF) .</p><p>Note — Phanera mekongensis is remarkable by its elongate laxly racemose inflorescences, flowers without a floral disc, anthers having a densely silky indumentum on the back and an ovary with a very short style and peltate stigma. Addition- ally, the morphological differences of the new species with its closely related species are shown in Table 2.</p></div>	https://treatment.plazi.org/id/0820A223DC26FF8E994EFF67FAE5FC54	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Mattapha, S.;Suddee, S.;Duangjai, S.;Kiewbang, W.	Mattapha, S., Suddee, S., Duangjai, S., Kiewbang, W. (2022): Phanera mekongensis (Fabaceae: Cercidoideae), a new species from Thailand as supported by morphological and molecular evidence. Blumea 67 (2): 113-122, DOI: 10.3767/blumea.2022.67.02.04, URL: https://doi.org/10.3767/blumea.2022.67.02.04
