taxonID	type	description	language	source
03AD7F1C4C1AFF87FF6EFE69FDD0A5AB.taxon	description	Mosquito females between 7 and 10 days old from both thewPip− and wPip + colonies were kept in groups of 100 individuals in paper cups and exposed to the infective blood meal using a Hemotek ® membrane feeding system for 1 h. After feeding, engorged females were selected following sedation with CO 2, transferred to new paper cups and maintained inside a climate chamber (Percival Scientific, Inc., Perry, IA, USA) maintained at 28 ° C and 70 % relative humidity RH, under 12: 12 - h light: dark cycle, with access to a 10 % sucrose solution. At days 7, 14 and 21 post-infection (dpi), 30 mosquitoes per group were anesthetized using CO 2,; the legs and wings were then removed and saliva was collected by inserting the mosquito proboscis into pipette tips filled with 5 µl of DMEM with 10 % FBS for 60 min. Te collected saliva was then transferred to a microtube containing 200 µl of DMEM supplemented with 10 % FBS. Te dissected body and legs plus wings were stored separately in microtubes containing 300 µl of complete DMEM with 10 % FBS, 1 % amphotericin B and three 2 - mm-diameter glass beads for subsequent homogenization during the RNA extraction. All samples were kept at − 80 ° C until further use in the RNA extraction.	en	Moura, Aires Januário Fernandes da, Tomaz, Filipe, Melo, Tiago, Seixas, Gonçalo, Sousa, Carla A., Pinto, João (2024): Vector competence of CUlex QUInQUefaSCIaTUS from Santiago Island, Cape Verde, to West Nile Virus: exploring the potential effect of the vector native WolbaCHIa. Parasites & Vectors (536) 17 (1): 1-12, DOI: 10.1186/s13071-024-06609-7, URL: https://doi.org/10.1186/s13071-024-06609-7
