identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03AD7F1C4C1AFF87FF6EFE69FDD0A5AB.text	03AD7F1C4C1AFF87FF6EFE69FDD0A5AB.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Culex quinquefasciatus Say 1823	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> Culex quinquefasciatus artificial infection with WNV </p>
            <p>Te infective blood meal was prepared by mixing blood and viral stock produced in the previous step to a final titer of 7.0 log 10 PFU/ml. An aliquot of the infectious blood meal was stored at − 80 °C for subsequent backtitration by plaque assay.</p>
            <p>Mosquito females between 7 and 10 days old from both thewPip− and wPip + colonies were kept in groups of 100 individuals in paper cups and exposed to the infective blood meal using a Hemotek ® membrane feeding system for 1 h. After feeding, engorged females were selected following sedation with CO 2, transferred to new paper cups and maintained inside a climate chamber (Percival Scientific, Inc., Perry, IA, USA) maintained at 28 °C and 70% relative humidity RH, under 12:12-h light:dark cycle, with access to a 10% sucrose solution.</p>
            <p>At days 7, 14 and 21 post-infection (dpi), 30 mosquitoes per group were anesthetized using CO 2,; the legs and wings were then removed and saliva was collected by inserting the mosquito proboscis into pipette tips filled with 5 µl of DMEM with 10% FBS for 60 min. Te collected saliva was then transferred to a microtube containing 200 µl of DMEM supplemented with 10% FBS. Te dissected body and legs plus wings were stored separately in microtubes containing 300 µl of complete DMEM with 10% FBS, 1% amphotericin B and three 2-mm-diameter glass beads for subsequent homogenization during the RNA extraction. All samples were kept at − 80 °C until further use in the RNA extraction.</p>
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	https://treatment.plazi.org/id/03AD7F1C4C1AFF87FF6EFE69FDD0A5AB	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Moura, Aires Januário Fernandes da;Tomaz, Filipe;Melo, Tiago;Seixas, Gonçalo;Sousa, Carla A.;Pinto, João	Moura, Aires Januário Fernandes da, Tomaz, Filipe, Melo, Tiago, Seixas, Gonçalo, Sousa, Carla A., Pinto, João (2024): Vector competence of CUlex QUInQUefaSCIaTUS from Santiago Island, Cape Verde, to West Nile Virus: exploring the potential effect of the vector native WolbaCHIa. Parasites & Vectors (536) 17 (1): 1-12, DOI: 10.1186/s13071-024-06609-7, URL: https://doi.org/10.1186/s13071-024-06609-7
