identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
039B878C9904FFD69BE3F9BD4FBAF7B4.text	039B878C9904FFD69BE3F9BD4FBAF7B4.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Dna extraction PCR	<div><p>DNA extraction, PCR amplification, and sequencing</p><p>For unculturable specimens, fungal material, including mycelium, conidiophores, and conidia, was directly harvested from the substrates and transferred into 1.5 mL Eppendorf tubes. In contrast, sufficient mycelium was collected from culturable specimens. DNA extraction was performed using the Trelief™ Plant Genomic DNA Extraction Kit (TSINGKE Biotech, Shanghai, China), following the manufacturer’s instructions. If the initial DNA extraction failed, the process was repeated thrice. Two barcodes, viz., the nuclear ribosomal internal transcribed spacer (ITS) region and the partial sequence of the large-subunit rRNA gene (LSU), were amplified by polymerase chain reaction (PCR). The pairwise primers were ITS9mun and ITS4_KYO1 (Toju et al. 2012) for ITS, LR0R (Vilgalys &amp; Hester 1990) and LR5 for LSU. The PCR reaction volume was 25 μL containing 2 μL of DNA template, 1 μL each of the forward and reverse primer (10 μM), 8.5 μL of double‐distilled water (ddH 2 O), and 12.5 μL of 2 × Flash PCR MasterMix (mixture of DNA Polymerase, dNTPs, Mg 2+ and optimized buffer; CoWin Biosciences, Taizhou, China). The PCR products were checked by agarose gel electrophoresis and then sent to Tsingke Biological Technology (Beijing, China) for Sanger sequencing. Based on the Sanger sequencing chromatograms, raw data were manually trimmed and assembled into consensus sequences using SeqMan Pro version 7.1.0 (DNASTAR, Inc. Madison, WI, USA).</p></div>	https://treatment.plazi.org/id/039B878C9904FFD69BE3F9BD4FBAF7B4	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Chen, Yanpeng;Liao, Yuechi;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Chen, Yanpeng, Liao, Yuechi, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Advancements in fungal and fungi-like diversity with a new species and updated records. Phytotaxa 701 (2): 127-147, DOI: 10.11646/phytotaxa.701.2.1, URL: https://doi.org/10.11646/phytotaxa.701.2.1
039B878C9902FFDF9BE3FF65493BFD92.text	039B878C9902FFDF9BE3FF65493BFD92.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Neomyrmecridium sichuanense Y. P. Chen & Maharachch. 2025	<div><p>Neomyrmecridium sichuanense Y.P. Chen &amp; Maharachch., sp. nov. (FIGURE 3)</p><p>Mycobank: MB 857089</p><p>Holotype: HUEST 24.0068</p><p>Etymology: The epithet refers to where the collection was made, Sichuan Province.</p><p>Saprobic on decaying twigs of an unidentified herbaceous plant. Sexual morph: Not observed. Asexual morph: Colonies on natural substrates effuse, scattered, or aggregated, hairy, brown, glistening. Conidiophores 93–130 × 4.0–5.0 (x = 115 × 4.5, n=20) macronematous, mononematous, erect, solitary or in small groups, lower part straight, upper part flexuous, cylindrical, reddish brown, slightly paler towards the apex, septate, unbranched. Conidiogenous cells polyblastic, integrated, terminal and intercalary, determinate, sympodial, denticulate, flexuous, cylindrical, pale reddish brown to subhyaline. Conidia acropleurogenous, solitary, fusiform or narrowly obovoid, subhyaline to pale brown, 0–1-septate, finely verrucose, 9.5–14.5 × 4.0–4.5 μm (x = 12 × 4.5 μm, n = 30).</p><p>Culture characteristics: Single-spore isolation was repeated three times on PDA and WA media, and no germinated spore was observed.</p><p>Material examined: CHINA. Sichuan Province, Chengdu City, Dujiangyan City, Longchi National Forest Park, 103°38’46 E, 31°0’11” N, elevation 702 m, on decaying branches of an unidentified herbaceous plant, 5 October 2021, Y.P. Chen and W.H. Tian, LC64 (HUEST 24.0068, holotype)</p><p>Notes: Our fungus exhibits the typical characteristics of Neomyrmecridium (Xu et al. 2023) . In the multi-gene phylogenetic tree (FIGURE 1), specimen HUEST 24.0068 forms a distinct branch. Its ITS sequence shows the highest similarity to Neomyrmecridium sorbicola CBS 143433, but with only 91% sequence identity (510/559, 8 gaps). Morphologically, HUEST 24.0068 and Neomyrmecridium sorbicola exhibit a similar conidial size dimension (7–15 × 4–5 μm), but the latter is characterized by the possession of appendages, a feature absent in HUEST 24.0068 (Crous et al. 2018). Therefore, the study proposed that the newly collected specimen be a new species to Neomyrmecridium, which is designated N. sichuanense .</p></div>	https://treatment.plazi.org/id/039B878C9902FFDF9BE3FF65493BFD92	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Chen, Yanpeng;Liao, Yuechi;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Chen, Yanpeng, Liao, Yuechi, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Advancements in fungal and fungi-like diversity with a new species and updated records. Phytotaxa 701 (2): 127-147, DOI: 10.11646/phytotaxa.701.2.1, URL: https://doi.org/10.11646/phytotaxa.701.2.1
039B878C990DFFDF9BE3FDF6494FFA02.text	039B878C990DFFDF9BE3FDF6494FFA02.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Paradiacheopsis longipes Hooff & Nann. - Bremek., Proc. K. Ned. Akad. Wet.	<div><p>Paradiacheopsis longipes Hooff &amp; Nann.-Bremek., Proc. K. Ned. Akad. Wet. 99(1-2): 51 (1996) (FIGURE 4)</p><p>Saprobic on dried twigs of an unidentified woody plant. Sexual morph: Not observed. Asexual morph: Sporangia stipitate, gregarious, globose and brown to dark brown, 380–450 μm high (x = 415 μm, n = 20). Stalk clustered, cylindrical, brown to dark brown, 1,150 –1,470 μm high (x = 1,339 μm, n = 20), upper stalk 60–145 μm (x = 103 μm, n = 20), and becoming wider at the base reaching to 315 μm. Columella thick and irregular, giving rise to primary branches of the capillitium along its entire length. Capillitium formed by brown threads frequently branching and intricately anastomosing, reaching the surface as long, straight, finely pointed horizontal free ends. Spores globose with verruculose protuberances, 5‒7.5 μm diam. (x = 6 μm, n = 30), pale brown.</p><p>Culture characteristics: Single-spore isolation was repeated three times on culture media (PDA and WA), and no germinated spore was observed.</p><p>Material examined: China, Sichuan Province, Chengdu City, Qionglai County, Nanmuxi, 103°7’16” E, 30°18’ N, elevation 775 m, on dried twigs of an unidentified woody plant, 24 August 2021, Y.P. Chen and Y.B Guo, NMX07 (HUEST 01.0275).</p><p>Notes: In the Index Fungorum (https://indexfungorum.org/Names/Names.asp, accessed January 10, 2025, Paradiacheopsis includes eight accepted species, all of which were described before the 21st century, with no molecular data available for any of their type materials. For our newly collected specimen, although we attempted to extract DNA directly from the fruiting bodies on natural substrates three times, all attempts were unsuccessful. Through a literature review, we compiled the spore characteristics of these eight species (TABLE 3). HUEST 01.0275 has pale brown spores, which are similar to those of Paradiacheopsis longipe, as well as in conidial size dimension (5‒7.5 μm vs. 7–8 µm diam.) (Hooff &amp; Nannenga-Bremekamp 1996). Therefore, we identified the newly collected specimen (HUEST 01.0275), as Paradiacheopsis longipes . The species was first reported by Ukkola et al. (2001) from Hunan Province, China, but no morphological data were provided. This study reidentifies the species in Sichuan Province and offers detailed characterization and visualization.</p></div>	https://treatment.plazi.org/id/039B878C990DFFDF9BE3FDF6494FFA02	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Chen, Yanpeng;Liao, Yuechi;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Chen, Yanpeng, Liao, Yuechi, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Advancements in fungal and fungi-like diversity with a new species and updated records. Phytotaxa 701 (2): 127-147, DOI: 10.11646/phytotaxa.701.2.1, URL: https://doi.org/10.11646/phytotaxa.701.2.1
039B878C990FFFDD9BE3FF0F4804FC5A.text	039B878C990FFFDD9BE3FF0F4804FC5A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Trimmatostroma salicis Corda, Icon.	<div><p>Trimmatostroma salicis Corda, Icon. fung. (Prague) 1: 9 (1837) (FIGURE 5)</p><p>Saprobic on decaying branches of an unidentified woody plant. Sexual morph: Not observed. Asexual morph: Colonies brownish to black, macroscopically visible, caespitose or in erect tufts. Mycelium internal, hyphae not very conspicuous, sparingly branched, pale brown to brown, septate, rather thick-walled, smooth. Stromata lacking. Conidiophores immersed, cylindrical, curved or upright, pale brown, 4–7 μm (x = 5.8 μm) wide, occasionally branched, hyphal filaments gradually developing into fertile threads by becoming somewhat wider and darker. Conidiogenous cells integrated, monoblastic, poorly differentiated, 3–7 × 3–6 μm (x = 5.4 × 4.6 μm, n= 30). Conidia in basipetal chains, multicellular, straight or curved long rod, 2–13-septate, 17–45 × 4–7 μm (x = 28.3 × 5.9 μm, n= 30), pale to dark brown, wall 0.3–0.8 μm (x = 0.6 μm) thick.</p><p>Culture characteristics: Conidia germinated on PDA within 12 h at 25 °C in darkness. Colonies white with a dense, uneven texture and a distinct brownish central area, with irregular and slightly raised margins, approximately 12 mm diam. on PDA after 4 weeks at 25°C; on the reverse side, bright orange, dense, and exhibits a radiating, slightly wrinkled texture with smooth and well-defined margins.</p><p>Material examined: China, Sichuan Province, Aba Tibetan and Qiang Autonomous Prefecture, Li County, Miyaluo Scenic Resort, 102°48’35” E, 31°42’56” N, elevation 3207 m, on decaying branches of an unidentified woody plant, 19 October 2021, Y.P. Chen and W.H. Tian, MYL57 (HUEST 24.0139), living culture UESTCC 24.0128.</p><p>Notes: BLASTn analysis showed that our newly collected isolate UESTCC 24.0128 displayed 99% sequence identity of ITS (661/663, 0 gaps), and 100% sequence identity of LSU to Trimmatostroma salicis CPC 13571. In the multilocus phylogenetic tree (FIGURE 2), isolate UESTCC 24.0128 cluster with T. salicis MFLU 18-0702 formed a strongly supported clade. Morphologically, the newly collected specimen resembles the generic type, Trimmatostroma salicis, in having much more compact and well-delimited sporodochia, up to 13-septate conidia and being non-lichenicolous (Ellis 1971, Crous et al. 2007). Therefore, we identified the newly collected specimen (HUEST 24.0139) as T. salicis, marking its first recorded occurrence in China.</p></div>	https://treatment.plazi.org/id/039B878C990FFFDD9BE3FF0F4804FC5A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Chen, Yanpeng;Liao, Yuechi;Shami, Ashwag;Alharbi, Nada K.;Liu, Jian-Kui;Maharachchikumbura, Sajeewa S. N.	Chen, Yanpeng, Liao, Yuechi, Shami, Ashwag, Alharbi, Nada K., Liu, Jian-Kui, Maharachchikumbura, Sajeewa S. N. (2025): Advancements in fungal and fungi-like diversity with a new species and updated records. Phytotaxa 701 (2): 127-147, DOI: 10.11646/phytotaxa.701.2.1, URL: https://doi.org/10.11646/phytotaxa.701.2.1
