Myrinia orieca Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 28

Myrinia orieca Grishin , new species

https://zoobank.org/ E284FB0F-A886-47D0-B039-E684CA8EF130

( Fig. 2 part, 61–62, 274–276)

Definition and diagnosis. Phylogenetic analysis reveals that specimens from eastern Ecuador identified as Myrinia binoculus (Möschler, 1877) (type locality in Suriname, syntype sequenced as NVG-15033C11) are genetically differentiated from it in both nuclear and mitochondrial genomes ( Fig. 2): e.g., their COI barcodes differ by 1.8% (12 bp), and therefore represent a new species. This new species keys to M. binoculus ((E.24.1) in Evans (1953) and differs from it by more elongated eyespot on the dorsal forewing, wider dark-brown forewing margins, better defined and more contrasting from the ground color bands (discal, postdiscal, and submarginal) on the ventral hindwing, and a smaller dark ventral hindwing tornal spot ( Fig. 61–62). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly2178.55.2:C48T, aly2178.55.2:C63T, aly1084.13.12:C93T, aly276558.43.4:A408G, aly4645.9.3:T103C, and COI barcode: A43G, A79G, T97C, A166G, A184G, A586G.

Barcode sequence of the holotype. Sample NVG-17105F03, GenBank OR837649, 658 base pairs: TACTTTATACTTTATTTTTGGAATTTGAGCAGGAATAGTAGGGACATCCTTAAGTTTATTAATTCGTACTGAATTAGGGAATCCAGGATCATTAATC GGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATGCCAATTATAATTGGAGGGTTTGGAAATT GACTTGTTCCATTAATATTAGGTGCCCCAGATATAGCTTTTCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCATCATTAATATTATTAAT TTCAAGAAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTACCCTCCTTTATCTGCTAATATTGCTCATCAAGGATCCTCAGTAGATTTA GCTATTTTTTCTTTACATTTAGCTGGAATTTCATCAATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGTATTAATAATCTTTCAT TCGATCAAATACCTTTATTTGTATGAGCAGTAGGAATTACAGCTTTATTATTACTATTATCTTTACCTGTATTAGCAGGAGCAATTACTATACTTTT AACGGATCGAAATTTAAATACATCATTTTTTGATCCTGCAGGTGGGGGAGATCCTATTTTATATCAACACTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History, Smithsonian Institution , Washington, DC, USA ( USNM), illustrated in Fig. 61–62, bears the following four rectangular printed labels, three white: [ ECUADOR: Orellana, | Tiputini Biodiversity Station, | Lower Río Tiputini, | 0 37′55″ S, 76 08′39″ W | 200 m, 10–15 Aug 2002 | J.P.W. Hall & M.A. Solis], [DNA sample ID: | NVG-17105F03 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 00894950], and one red [HOLOTYPE ♂ | Myrinia | orieca Grishin ] . Paratype: 1♂ NVG-14112H01 Ecuador: Napo, Yasuní National Park, 20-Sep–4-Oct-2003, C. Bordelon and E. Knudson, the Texas Lepidoptera Survey collection [ MGCL].

Type locality. Ecuador: Orellana Province, Lower Río Tiputini, Tiputini Biodiversity Station, elevation 200 m, GPS −0.6319, −76.1442.

Etymology. This species comes from the Ori [ente (eastern region) in] Ec [u] a [dor]. The name is a Latinized feminine adjective.

Distribution. Currently known only from the Amazonian region in eastern Ecuador.