Aguna lata Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 16-17

Aguna lata Grishin , new species

https://zoobank.org/ 1D86CDBD-1D55-4A24-9705-84847DF9E86B

( Fig. 1 part, 31–32, 243–245)

Definition and diagnosis. Inspection of genomic trees reveals that a specimen identified as Aguna spatulata Austin and O. Mielke, 1998 (type locality in Brazil: Rondônia) is sister to the clade consisting of A. spatulata , Aguna panama Austin and O. Mielke, 1998 (type locality in Panama), and Aguna similis Austin and O. Mielke, 1998 (type locality in Brazil: Rondônia) and therefore is a distinct species. The new species differs from its closest relatives by a more angular distal margin of lamella antevaginalis, with slightly concave lobes on the sides of the notch ( Fig. 243); these lobes appear truncate rather than rounded as in other species ( Austin and Mielke 1998). Due to the cryptic nature of this species and unknown males, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly37338.40.1:A567G, aly 1651.2.15:T844C, aly 1651.2.15:T1098C, aly1146.14.5:T39A, aly318.14.14:T432C, aly806.11.5:G1380G (not A), aly383.14.5:C90C (not T), aly114.6.2:T960T (not C), aly770.33.9:A75A (not G), aly725.4.3:A123A (not G), but the COI barcode does not distinguish this species from A. spatulata .

Barcode sequence of the holotype. Sample NVG-18016D11, GenBank OR837635, 658 base pairs: AACTTTATATTTTATTTTTGGTATTTGAGCAGGATTAGTAGGAACTTCTTTAAGATTATTAATTCGAACTGAATTAGGAACACCAGGATCTTTAATT GGAGATGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATT GACTAGTACCCCTTATACTAGGAGCTCCTGATATAGCATTCCCCCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAACCCTTTTAAT TTCTAGTAGTATCGTAGAAAATGGTGCAGGCACAGGATGAACAGTTTACCCCCCACTTTCATCTAATATTGCCCATCAAGGAGCTTCAGTTGATTTA

GCAATTTTCTCCTTACACTTAGCAGGAATTTCTTCTATTCTTGGAGCTATTAATTTTATCACCACAATTATTAACATACGAATTAATAATATATCAT TTGATCAAATATCATTATTTATTTGAGCTGTTGGAATTACAGCTTTATTATTACTACTATCTTTACCAGTTTTAGCAGGAGCTATTACAATATTATT AACAGATCGAAATTTAAATACATCATTTTTTGATCCTGCTGGAGGGGGAGATCCTATTCTTTACCAACATTTATTC

Type material. Holotype: ♀ deposited in the National Museum of Natural History , Smithsonian Institution, Washington, DC, USA ( USNM), illustrated in Fig. 31–32, bears the following four rectangular labels, three white: [ GUYANA: Trop F Res | Middle Demerara R | 200–400′ | 31.I-12.II.2001 | 5°9.32′N 58°41.98′W | Leg. S.Fratello et al], [DNA sample ID: | NVG-18016D11 | c/o Nick V. Grishin], [{QR Code} | USNM ENT 00234359 ], and one red [HOLOTYPE ♀ | Aguna | lata Grishin ].

Type locality. Guyana: Potaro-Siparuni Region, middle Demerara River, Tropenbos forest reserve, elevation 200–400′, GPS 5.1553, −58.6997.

Etymology. The name is formed from its similar species, [spatu] lata , and is a noun in apposition.

Distribution. Known only from the holotype collected in Guyana.