Abronia cunemica Clause, Luna-Reyes, Mendoza-Velázquez, Nieto-Montes de Oca & Solano-Zavaleta, 2024
|
publication ID |
https://doi.org/ 10.1371/journal.pone.0295230 |
|
persistent identifier |
https://treatment.plazi.org/id/038187B4-FFFD-FF8B-7C6E-FA8CFB827CAC |
|
treatment provided by |
Felipe |
|
scientific name |
Abronia cunemica Clause, Luna-Reyes, Mendoza-Velázquez, Nieto-Montes de Oca & Solano-Zavaleta |
| status |
sp. nov. |
Abronia cunemica Clause, Luna-Reyes, Mendoza-Velázquez, Nieto-Montes de Oca & Solano-Zavaleta sp. nov.
urn:lsid:zoobank.org:act:5E56D78F-1DEF-42B3-AEBD-B2AD2E1D1817
Dragoncito de Coapilla (recommended Spanish common name)
Coapilla Arboreal Alligator Lizard (recommended English common name)
Figs 5–8 View Fig 5 View Fig 6 View Fig 7 View Fig 8 , Table 1 View Table 1 .
Holotype ( Figs 5–8 View Fig 5 View Fig 6 View Fig 7 View Fig 8 , Table 1 View Table 1 )
MZFC-HE 36544 (field series number AGC 1428), adult male, vicinity of Coapilla , Municipio de Coapilla, Northern Highlands, Chiapas, Mexico (17.14˚, -93.16˚, datum WGS 84), 1625 m elevation, Adam G. Clause, Emmanuel Javier-Vázquez, Ana Reyna Pale Morales, 14 August 2021 GoogleMaps .
Paratypes ( Figs 6 View Fig 6 and 7 View Fig 7 , Table 1 View Table 1 , n = 4)
MZFC-HE 36545 (field series number AGC 1429), adult female, all collection data the same as for the holotype. GoogleMaps MZFZ 4512 (AGC 1484), juvenile male, vicinity of Coapilla , Municipio de Coapilla, Northern Highlands, Chiapas, Mexico (17.13˚, -93.16˚, datum WGS 84), 1605 m elevation, Oscar M. Mendoza-Velázquez, Candelario Cundapí-Pérez, Roberto Luna-Reyes, Adam G. Clause, Marcos Joaquín Fitz-Pérez, José Manuel Toledo-Morales, Emmanuel Javier-Vázquez, Daniel Lara-TufiÑo, 16 February 2022. GoogleMaps MZFZ 4513–4514 ( AGC 1491–1492), two adult females, all collection data the same as for MZFZ 4512 except collected on 18 and 19 February 2022, respectively. The GPS coordinates in this paragraph and the preceding paragraph are intentionally imprecise; details are available in the Conservation subsection GoogleMaps .
Diagnosis
Abronia cunemica sp. nov. can be distinguished from all recognized congeners, including all species formerly considered members of the genus Mesaspis , by the following combination of characters: (1) lack of protuberant or spine-like supra-auricular scales; (2) lack of protuberant or casque-like posterolateral head scales; (3) dorsum of head pale yellow with distinct dark markings; (4) 35–39 transverse dorsal scale rows; (5) lateralmost row of ventral scales enlarged relative to adjacent medial row; and (6) dorsum brown with dark crossbands, these sometimes reduced to series of dark spots.
Comparisons
Abronia cunemica sp. nov. can be differentiated from all species of the former genus Mesaspis by having 35–39 transverse dorsal scale rows (vs.> 40 rows). Additionally, the new species differs from A. cuchumatanus , A. gadovii , and the A. moreletii species complex by having 14 longitudinal dorsal scale rows (vs. 16 or 18, 16–18, and 18–22, respectively); from A. antauges and A. juarezi by having vertebral and paravertebral dorsal scales strongly keeled at midbody (vs. smooth to slightly convex); from A. viridiflava by having the frontonasal scale present (vs. absent); and from A. monticola by having a divided or partly divided postmental scale in 3/5 or 60% of specimens (vs. undivided). Among Abronia species not previously considered members of the former genus Mesaspis , Abronia cunemica sp. nov. differs from each recognized subgenus as follows, with character state(s) for each subgenus in parentheses and subgeneric synapomorphies (if they exist) indicated in italic font. Unlike the subgenus Scopaeabronia , the new species has the lower primary temporal scale unexpanded (vs. expanded), 6 longitudinal nuchal scale rows (vs. 8 rows) and 35–39 transverse dorsal scale rows (vs. 38–47). Unlike the subgenus Auriculabronia , the new species lacks strongly protuberant or spine-like supra-auricular scales (vs. present). Within the subgenus Auriculabronia , the new species further differs from A. matudai (a species in which the supra-auricular scales are sometimes barely protuberant) in having a dark lateral bar on the neck present, albeit often divided into two separate blotches (vs. absent); and in having a dorsum that is brown with dark crossbands, albeit often reduced to series of spots (vs. dorsum green with no crossbands in adult males). Unlike the subgenus Abronia , the new species has the lateralmost row of ventral scales distinctly enlarged relative to the adjacent medial row (vs. not enlarged). Within the subgenus Abronia , the new species further differs from the deppii group ( A. cuetzpali , A. deppii , A. martindelcampoi , A.
https://doi.org/10.1371/journal.pone.0295230.t001
mixteca , and A. oaxacae as defined by Campbell et al. [ 84], contra Campbell and Frost [ 39]) in having dorsal scales on the flanks arranged in parallel longitudinal rows relative to the ventrolateral fold (vs. oblique longitudinal rows), and from A. zongolica by having 35–39 transverse dorsal scale rows (vs. 30–34). Unlike the subgenus Aenigmabronia , the new species has one occipital scale (vs. two occipitals), and two scale rows separating the occipital scale from the first transverse nuchal scale row (vs. one scale row separating the occipitals from the nuchals). Unlike the subgenus Abaculabronia , the new species has a brown dorsum with at least traces of crossbands in life in adults (vs. dorsum olive green with pale or yellow scale margins and no trace of crossbands in life in adults), and supranasal scales lacking contact in all specimens (vs. supranasals in contact in 8/9 or 89% of specimens). Within the subgenus Lissabronia , no single character differentiates the new species from all four currently recognized members of the group. However, it can be distinguished from all Lissabronia species except for A. morenica by having 35–39 transverse dorsal scale rows (vs. 28–32). Unlike A. morenica , the new species attains a larger adult size of 107–127 mm snout-to-vent length (vs. 92–93 mm), and the dorsum of the head is pale yellow with distinct dark markings (vs. pale gray or tan with dark markings absent or faint). Table 1 View Table 1 presents additional characters that differentiate species of Lissabronia from A. cunemica sp. nov. and/or from each other.
We are uncertain about the subgenus to which A. cunemica sp. nov. belongs. Nonetheless, we provisionally assign it to Lissabronia based on our phylogenetic analysis (see above). Furthermore, unlike for all other subgenera, no single character cleanly separates the new species from Lissabronia based on the diagnosis provided by Campbell et al. [ 1] and on the subsequent description of A. morenica [ 44] that provisionally assigned that species to Lissabronia . In the Discussion section we present further explanation for our tentative assignment of the new species.
Description of holotype ( Figs 5–8 View Fig 5 View Fig 6 View Fig 7 View Fig 8 , Table 1 View Table 1 )
Adult male with both hemipenes everted, mass 37.6 g, snout-to-vent length (SVL) 127 mm, head length from rostral to upper anterior edge of ear opening 29 mm, head width at broadest point 22 mm, head width/length ratio = 75.9%, tail broken and regenerating, tail length 121 mm, and 50 caudal whorls (including regenerating portion).
Supranasals 1/1, neither expanded medially; postnasals 2/2, upper smaller than lower; one pair each of anterior and posterior internasals situated between rostral and frontonasal, right anterior internasal abnormally divided into a large medial and a small lateral scale; prefrontals>2 times size of posterior internasals, broadly contacting each other medially; canthals 1/1, separating posterior internasal and prefrontal; loreals 1/1, in contact with both postnasals; cantholoreals 1/1, barely extending onto dorsum of canthus rostralis, narrowly contacting anterior median supraocular, broadly contacting canthal, prefrontal, and supralabials; median supraoculars 5/5; lateral supraoculars 3/3; superciliaries 6/6, anteriormost contacting cantholoreal and <1.5 times length of adjoining superciliary; preoculars 1/1, left fused with anterior subocular; suboculars 1/2, anterior on left aberrantly fused with preocular; posterior suboculars not contacting lowermost primary temporal; postoculars 2/3; frontal large, not contacting frontonasal, narrowly contacting interparietal; parietals lacking contact with median supraoculars; one large occipital, slightly larger than interparietal; two transverse scale rows separating occipital from first transverse row of nuchals; primary temporals 4/4, only lowermost two contacting postoculars on each side, third aberrantly fused with fourth secondary temporal on each side; secondary temporals 4/4, fourth aberrantly fused with third primary temporal on each side; tertiary temporals 4/5; supralabials 11/10, antepenultimate posteriormost to reach orbit; infralabials 8/8; postmental divided; three pairs of enlarged chin shields posterior to divided postmental, posteriormost ones subequal in size to adjacent chin shields,> 1.5 times size of posterior scales; sublabials 5/5, anteriormost contacting second infralabial but not postmental.
Minimum longitudinal nuchal scale rows 6; transverse dorsal scale rows 36–38; longitudinal dorsal scale rows 14, arranged in parallel horizontal rows on sides of body; eight middorsal longitudinal scale rows strongly keeled, becoming smooth on flanks; transverse ventral scale rows 37; longitudinal ventral scale rows 12; lateralmost row of ventral scales enlarged relative to adjacent medial row, but only on middle third of body; osteoderms appear moderately well developed on head and adjacent nuchals; posterolateral corners of head not protruding or casque-like; osteoderms appear weakly developed or absent on body and tail; supra-auricular scales granular, not protruding or spine-like; scales on side of neck between enlarged lateral nuchals and enlarged ventrolateral scales (hereafter, lateral neck scales) 8–10, granular; antebrachials from insertion of forelimb to wrist 12–13; ventrolateral fold between ear opening and forelimb absent; ventrolateral fold posterior to forelimb moderately well developed, with 3–6 indistinct longitudinal rows of small scales and granules on interstitial skin; subdigital lamellae on fourth toes 19/19.
Coloration of holotype
In life, body medium ochre brown becoming pale yellow on lateral nuchals and dull lemon yellow with brown flecks on flanks. Body with distinct dark brown flank blotches and faint dark brown middorsal spots, seemingly the remnants of 10 indistinct crossbands. Dark blotches and spots separated by 3 scales middorsally and 1.5–2.5 scales laterally. Most scales in and along ventrolateral fold dull lemon yellow, but some mostly or entirely reddish brown due to ventrolateral continuation of crossbands. Lateral neck scales mostly whitish to dull lemon yellow, with 3–4 distinct reddish-brown ventrolateral blotches. Lateral neck scales bordered dorsally by prominent, interrupted or nearly interrupted black to dark brown bar extending from shoulder to near upper posterior edge of ear opening, 1–2 times width of lowermost nuchal scales. Dorsal surface of forelimbs and hindlimbs rusty brown, with a few yellow highlights and dark brown flecking and blotching, more so on forelimbs. Forelimb digits dull yellow with rusty brown spotting, hindlimb digits rusty brown. Head pale yellow becoming pale gray to white laterally with prominent, ill-defined arrowhead-shaped black to dark brown dorsal blotches. Head scales with moderate rugosity accentuated by fine black to dark brown flecking and vermiculations that extend onto most nuchals. Scales between nares on dorsum, and between orbit and rostral on sides of head (except cantholoreal), with no dark flecking or vermiculations. Tail same color as body, with 11 indistinct but mostly uninterrupted dark brown to blackish crossbands that degrade into a checkerboard-like pattern ventrally. Lower jaw, chin, and throat white to pale gray, many scales with distinct dark flecking on anterior or dorsal margins, especially prominent on sublabials and chinshields. Venter anterior to forelimbs white to pale gray, becoming yellow on remainder of venter including hindlimbs and base of tail, and beige with faint yellow tinge on remainder of tail. Many ventral scales with prominent pale ochre brown markings, becoming more extensive laterally. Manus and pes rusty orange to rusty yellow. Iris pale yellow with heavy dark flecking.
In preservative (ethanol after formalin), body medium ochre brown with small parts of some scales becoming greenish, cream on lateral nuchals and horn-colored with brown flecks on flanks. Body with distinct brown flank blotches and faint brown dorsal spots. Most scales in and along ventrolateral fold horn-colored, but some mostly or entirely brown due to ventrolateral continuation of crossbands. Lateral neck scales mostly whitish to horn-colored, with 3–4 distinct greenish brown ventrolateral blotches. Lateral neck scales bordered dorsally by prominent, interrupted or nearly interrupted dark brown bar extending from shoulder to near upper posterior edge of ear opening. Dorsal surface of forelimbs and hindlimbs olive, with a few cream highlights and light brown flecking and blotching, more so on forelimbs. Fingers horn-colored to whitish with light brown spotting, toes light brown to olive. Head pale gray becoming white laterally with prominent, vaguely arrowhead-shaped brown dorsal blotches. Head scales with moderate rugosity accentuated by fine brown flecking and vermiculations that extend onto most nuchals. Tail lighter than body, with mostly light brown to brown crossbands that fade to horn-colored ventrally. Lower jaw, chin, and throat white to cream. Venter anterior to forelimbs white, becoming mostly cream and horn-colored laterally over the remainder of venter including limbs and base of tail, and grayish-cream on remainder of tail. Manus and pes saffron.
Variation ( Figs 6 View Fig 6 and 7 View Fig 7 )
All four paratypes are similar to the holotype in most respects, but they differ as follows. Three adult females (MZFC-HE 36545 and MZFZ 4513–4514) with mass 22.1–24.3 g (X � ¼ 23:4), SVL 107–113 mm (X � ¼ 110), head length from rostral to upper anterior edge of ear opening 22–23 mm (X � ¼ 22:3), head width at broadest point 16–17 mm (X � ¼ 16:3), head width/ length ratio 72.7–73.9% (X � ¼ 73:1%), tail unbroken and unregenerated only in MZFC-HE 36545, with tail length 156 mm (1.46 times SVL) and 97 caudal whorls. One juvenile male (MZFZ 4512) with mass 13.9 g, SVL 91 mm, head length 18 mm, head width 12 mm, head width/length ratio 66.7%, tail broken and regenerating, tail length 126 mm (including 26 mm regenerated tissue).
Left supranasal expanded medially and displacing adjacent posterior internasal in MZFC-HE 36545, and left supranasal damaged in MZFZ 4514; medial azygous scale present between anterior and posterior internasals in MZFZ 4514; prefrontals 2 times size of posterior internasals in MZFZ 4512, 2.5 times size of posterior internasals in MZFC-HE 36545 and MZFZ 4513–4514, but not contacting each other in MZFC-HE 36545 and narrowly contacting each other in MZFZ 4513; cantholoreal not (left) or barely (right) contacting anterior median supraocular in MZFC-HE 36545, no median supraocular contact in MZFZ 4513, and very narrow contact in MZFZ 4514; superciliaries 7/ 7 in MZFC-HE 36545, MZFZ 4512, and MZFZ 4513; anteriormost superciliary nearly 2 times length of adjoining superciliary in MZFC-HE 36545 and MZFZ 4513, and nearly same length as adjoining superciliary in MZFZ 4512; suboculars 2/ 3 in MZFZ 4514, right anteriormost aberrantly divided into small posterior and large anterior scales, and 2/2 on all other paratypes; posterior suboculars not contacting lowermost primary temporal in all paratypes; postoculars 4/ 4 in MZFC-HE 36545 and MZFZ 4514, 3/ 3 in MZFZ 4512, and 3/ 4 in MZFZ 4513; frontal narrowly contacting frontonasal in MZFC-HE 36545, barely contacting frontonasal via thin spur in MZFZ 4512 and MZFZ 4514; occipital smaller than interparietal in all paratypes, abnormally divided posteriorly to form a small semi-triangular scale on left side in MZFC-HE 36545 and right side in MZFZ 4513; two transverse scale rows separating occipital from nuchals with enlarged posteriormost scale medially dividing first row of nuchals in MZFZ 4513; third primary temporal aberrantly fused with uppermost secondary temporal on each side only in MZFC-HE 36545; secondary temporals 3/ 4 in MZFZ 4512 and MZFZ 4513, second on left and third on right abnormally enlarged posteriorly and partially displacing adjacent tertiary temporals in MZFZ 4512, first and second on left separated by abnormally enlarged second tertiary temporal in MZFZ 4513, second on left abnormally small in MZFZ 4514; tertiary temporals 4–5 (X � ¼ 4:6), second abnormally enlarged and anteriorly displaced in MZFZ 4513; supralabials 10–11 (X � ¼ 10:3); infralabials 8–9 (23345076), small aberrantly divided sublabial nearly separating eighth and ninth sublabials on right side in MZFZ 4514; postmental single in MZFZ 4513, partly divided posteriorly in MZFC-HE 36545 and anteriorly in MZFZ 4514; second sublabial on left abnormally divided in MZFZ 4513, fifth sublabial on both sides aberrantly divided and anteriormost contacting second (left) or third (right) infralabial in MZFZ 4514, sublabials 4/5 with anteriormost contacting third (left) or second (right) infralabial in MZFZ 4512.
Transverse dorsal scale rows 35–39 (X � ¼ 37:25); eight middorsal longitudinal scale rows strongly to moderately keeled in MZFZ 4512 and MZFZ 4514; transverse ventral scale rows 37–40 (X � ¼ 38:5); lateral neck scales 8–9 in MZFC-HE 36545, 9–10 in MZFZ 4513–4514; subdigital lamellae on fourth toes 18–21 (X � ¼ 19:6).
In life, the three adult female paratypes (MZFC-HE 36545 and MZFZ 4513–4514) differ from the adult male holotype as follows: body dark brown with rusty tinge (MZFZ 4513) to pale ochre brown (MZFZ 4514); flanks medium rusty brown (MZFZ 4513) to pale brown (MZFZ 4514), becoming pale gray, beige, or cream anteriorly; remnants of dark crossbands even more reduced on MZFC-HE 36545, but crossbands unbroken, distinct, and roughly chevron-shaped on MZFZ 4513–4514, separated by 0.5–2 scales middorsally and 1.5–3.5 scales laterally; scales in and along ventrolateral fold same color as adjacent flank scales, including ventrolateral continuation of darker crossbands; lateral neck scales whitish to pale gray, rarely with yellow tinge, and ventrolateral blotches blackish to medium brown; dark lateral neck bar interrupted in MZFC-HE 36545, complete in MZFZ 4513–4514; dorsal surface of forelimbs and hindlimbs whitish, pale gray, pale brown, medium brown, or rusty brown, often with darker flecks or blotches and with forelimbs always at least partially paler than hindlimbs; digits various shades of gray or brown, often with darker spots or markings, rarely with cream flecks or highlights; 4–5 alternating brown spots on supralabial and/or infralabial scales in MZFC-HE 36545 and MZFZ 4513; cantholoreal scales lack dark flecks and vermiculations; tail with 9 (MZFC-HE 36545, tail broken and not yet regenerating), 14 (MZFZ 4514, tail regenerating), or 22 (MZFZ 4513, tail unbroken) dark brown crossbands, some partially merged with adjacent crossbands; regenerated portion of tail in MZFZ 4514 almost completely dark brown; lower jaw, chin, and throat lack dark flecking; venter white to pale gray becoming darker gray laterally, with pale yellow or cream midventral stripe posterior to forelimbs; venter of tail white to pale gray with degraded crossbands forming ill-defined checkerboard-like pattern of darker gray blotches; manus and pes bright rusty orange to dull rusty brown, sometimes becoming yellowish basally; iris with faint green tinge in MZFC-HE 36545.
In life, the juvenile male paratype (MZFZ 4512) differs from the adult male holotype as follows: body pale brown becoming paler beige on flanks, with some darker flecking throughout and a faint cream tinge anteriorly; body with 11 indistinct dark brown crossbands, largely reduced to blotches on vertebral and paravertebral scale rows, often bordered posteriorly with pale gray to beige flecks or spots, each separated by 2–3 scales both middorsally and laterally; most scales in and along ventrolateral fold beige, but some mostly dark brown bordered posteriorly by white or pale gray spots due to ventrolateral continuation of crossbands; lateral neck scales whitish, some with cream tinge, and ventrolateral blotches medium brown; dark lateral neck bar interrupted; forelimbs and digits beige to pale brown with medium brown flecks and spots; hindlimbs and digits medium brown with dark brown flecks and spots; 3–4 alternating pale brown spots on supralabial scales; pale yellow and dark brown markings on dorsum of head faded and less contrasting; head scales smooth or weakly rugose, with less extensive dark flecking and vermiculations; tail slightly paler than body, especially posteriorly, but dark crossbands more distinct than on body; regenerated portion of tail pale brown with darker flecking and no crossbands; lower jaw, chin, and throat lack dark flecking; venter anterior to forelimbs white to pale gray, becoming pale yellow on remainder of venter including limbs and base of tail, and beige to pale gray on remainder of tail; manus and pes dull yellow-orange.
In preservative (ethanol after formalin), all paratypes maintain their basic color pattern elements from life except that rusty brown tones are mostly replaced by various shades of tan, cream, or pale gray; dorsum of body dark brown to pale gray or pale brown, but scales in lighter-colored paratypes with greenish tones; dark dorsal crossbands slightly faded and less distinct; neck generally more grayish, tan, or pale brown; dorsum of head grayish; head markings accentuated and distinct in all specimens; faint to distinct oblique dark mark extends from posterior margin of frontal to tertiary temporals in all specimens; manus and pes yellowish to saffron.
Etymology
The species name is a feminine singular adjective in the nominative case derived from CuÑemo (alternative spellings: KuÑØmØ or Kujnyä’mä), which is the name for Coapilla in the indigenous Zoque language. Coapilla is derived from the Náhuatl words coatl (snake) and apan (river) and means “river of the snakes,” while CuÑemo has been variously translated as agua entre los árboles (“water among the trees” in English) [ 85], lugar de la gran capital (“place of the new or great capital”) [ 86], or corona de cerros (“crown of hills”) according to residents of the area. The chosen name, derived from the Zoque language, thus refers to the ejido and municipality which support the only known population of the new species. Our inquiries with residents of Coapilla about this name received universally positive responses. The recommended English pronunciation is “koon-YEM-ih-kuh.”
Distribution and ecology
Like most arboreal Abronia species described in the last 30 years, A. cunemica sp. nov. has only been reported from the vicinity of the type locality ( Fig 8 View Fig 8 ). However, also like many other members of the genus, this species probably occurs more widely given the presence of seemingly suitable interconnected highland forests. All documented populations exist on a small, wooded tableland. To the north and east, this tableland transitions into steep slopes that form several peaks exceeding 2200 m elevation which overlook the town of Tapalapa. To the south and west, the tableland ends sharply in a long bank of dramatic cliffs and promontories that loom above the town of Copainalá. Rainfall on this tableland drains either into the Río Zacalapa to the west or the Río Chavarria to the east. Both waterways then feed into the Gulf of Mexico, as part of the Río Grijalva basin.
The woodland in and around the type locality for A. cunemica sp. nov. is pine-oak forest, and includes Pinus chiapensis , P. maximinoi , Quercus crispipilis , and Q. peduncularis ( Fig 9B View Fig 9 ). Although uncommon, Carpinus caroliniana and Liquidambar styraciflua trees are also present. Epiphytic growth in occupied forest is variable ( Fig 9A and 9C View Fig 9 ). In some patches it is extensive, dominated by dense masses of Tillandsia fasciculata and/or T. rodrigueziana . Other, less abundant bromeliads include T. juncea , T. comitanensis and/or T. makoyana , and an unidentified species of Catopsis . Many trees support Toxicodendron radicans vines, which at times form large tangles that ascend high into the canopy. Less conspicuous elements of the epiphytic flora include at least one species of mistletoe, a few species of ferns, and several orchids that include Domingoa purpurea and one or more species in the genera Epidendrum or Prosthechea . Epiphytic moss is generally scarce or absent, but branches of many trees are laden with crustose, foliose, and fruticose lichens in the genera Cladonia , Leptogium , Parmotrema , Pseudocyphellaria , and Usnea [ 87].
On five separate trips to the vicinity of Coapilla (22–23 August 2015, 9–10 August 2021, 13– 15 August 2021, 15–19 February 2022, and 5–7 August 2022), we cumulatively invested over 350 person-hours of search effort specifically for A. cunemica sp. nov. in occupied habitat, including climbing with ropes into the canopy of almost 20 trees to search among the epiphytes. Despite this effort, we found only five individuals. This difficulty in encountering the species was echoed in our conversations with local residents. Of approximately two dozen people that we met while walking trails and roads through occupied forest, only about half of them recognized the Abronia when shown a photograph, video, or live animal. Furthermore, a student who completed extensive herpetological fieldwork in the Municipality of Coapilla, including one site that overlaps the type locality of A. cunemica sp. nov., reported no observations of the species [ 88].
The arboreal behavior of A. cunemica sp. nov., together with the dense epiphytic growth in many trees of the region, are likely responsible for this lack of familiarity and few observations. We found three specimens 3.5–19.5 m high on limbs of both live and dead Pinus chiapensis trees. We also found a pair in a courtship bite-hold on the forest floor, after they presumably fell from an adjacent Quercus sp. tree. We observed individuals from 10:30–13:30 hr, during sunny or partly cloudy conditions with ambient air temperatures of 20–27˚C. Consistent with the summer/fall mating season documented in other Abronia species [ 89 – 91], we found the courting pair of A. cunemica sp. nov. on 14 August. Although the male immediately released his bite hold on the female upon capture, a few hours later when returned to close contact with her, he re-initiated a more persistent bite hold on her head and neck. After being manually separated, a few minutes later we again brought the pair into close contact. The male responded with repeated bouts of subtle yet intense tail tremors lasting several seconds. Two adult females subsequently collected in mid-February appeared to be gravid, although we did not dissect the specimens to confirm this suspicion.
The male holotype of Abronia cunemica sp nov. is unusually large, measuring 127 mm SVL. Among arboreal Mexican species of the genus, only A. mixteca is reported to be larger, with a maximum known SVL of 148 mm [ 39]. The Guatemalan endemic species A. anzuetoi and A. fimbriata are the only other species known to exceed A. cunemica sp. nov. in size, attaining lengths of 143 and 130 mm SVL, respectively [ 39, 92]. However, we are aware of unpublished records of A. lythrochila exceeding 127 mm SVL, and we hope to see this data formally announced soon.
Remarkably, one adult female paratype (MZFZ 4514) of A. cunemica sp. nov. was re-found and collected 97 days after she was captured, photographed, and released by OMMV on 14 September 2021. Her location when re-captured was less than 10 m from her original capture location, in a Pinus chiapensis tree that was adjacent to the tree of the same species where she was originally found. Albeit limited, these data suggest that A. cunemica sp. nov. can be relatively sedentary at least some months of the year.
Based on our field sampling, arboreal or semi-arboreal herpetofauna with which A. cunemica sp. nov. occurs in sympatry (within 2 airline km) include Sceloporus internasalis (MZFZ 4477), Imantodes gemmistratus (MZFZ 4562), Bolitoglossa rufescens (MZFZ 4473), Bromeliohyla bromeliacia (MZ-UNICACH 68–79) [ 93], and Smilisca baudinii (MZFZ 4561, 4565). One or more of these species might be a food source for the new species, and I. gemmistratus could conceivably eat young A. cunemica sp. nov. Including previous records [ 88], we also documented sympatric co-occurrence of the following reptiles: Anolis tropidonotus (= A. spilorhipis or A. t. spilorhipis of some authors; MZFZ 4559–4560), Scincella incerta (MZFZ 4472), Drymobius margaritiferus (MZ-UNICACH 194) [ 88], Geophis carinosus (MZFZ 4475–4476), Ninia diademata (MZ-UNICACH 196, 376), Pituophis lineaticollis (MZ-UNICACH 198) [ 88], Rhadinaea [= Rhadinella] godmani (MZFC-HE 29997) [ 94], Tantilla schistosa MZFZ 4471, Thamnophis cyrtopsis (MZ-UNICACH 378) [ 88], Micrurus elegans (MZFZ 4474); and the following amphibians: Incilius valliceps (MZ-UNICACH 95–98) [ 88], Craugastor lineatus (MZ-UNICACH 454) [ 88], C. pygmaeus (MZFZ 4563–4564), and Rana brownorum (MZ-UNICACH 155–157) [ 88]. Further sampling will likely show A. cunemica sp. nov. to co-occur with the terrestrial congener Abronia temporalis , which has been collected less than 8 km to the northeast (MVZ 272336).
Conservation
Abronia cunemica sp. nov. is unrecorded from any protected areas. However, several forest parcels within a 5 km radius of the type locality have been designated as small reserves by residents of Coapilla. These patches could support populations of the species. Upon touring one such parcel, we found a more intact forest canopy compared to other lands that we visited, but the understory was highly modified by widespread cutting of saplings (probably for firewood). The Parque Ecoturístico Laguna Verde is another area managed by the Ejido of Coapilla where A. cunemica sp. nov likely occurs, and where relatively environmentally friendly ecotourism activities are promoted by the community. However, socioeconomic problems that are well-documented in other protected areas of Chiapas also affect this ecotourism park and its surroundings [ 95 – 98]. Lastly, an additional 101-ha protected area called the Zona Sujeta a Conservación Ecológica Tzama Cum Pümy, which lies some 7 km from the type locality of A. cunemica sp. nov., could also support the species [ 99 – 101]. Future surveys, in close partnership with community leaders in Coapilla and Tapalapa, are necessary to confirm these suspicions.
The forest on the tableland where A. cunemica sp. nov. lives is generally perturbed to some degree (e.g., Fig 9A View Fig 9 ). Much of the landscape has been converted to a savannah, with widelyspaced trees and a grassy understory with few shrubs and saplings. On multiple occasions, we also observed trucks loaded with large freshly-cut pine logs leaving the area. Within 100 m of all sites where we found A. cunemica sp. nov., we saw agricultural, livestock, and forestry activities taking place. The absence of intact forest and ongoing loss of trees are nonetheless tempered by three factors. First, many communally-owned parcels in the Ejido of Coapilla are under active forest management. Based on public signage that we observed, this management approach appears to disallow clear-cuts in favor of selective logging on a cyclical basis across a mosaic of designated parcels. Second, our surveys revealed that adult female and juvenile A. cunemica sp. nov. can live in remnant trees growing within pastureland. The species thus might have substantial tolerance to forest disturbance, similar to reports for a few other arboreal species of Abronia [ 102]. Third, individuals of A. cunemica sp. nov. appear to spend most of their lives hidden in the forest canopy, where they are inherently sheltered from direct human influence. For these reasons, despite ongoing forest loss that clearly eliminates habitat for this species and imperils its survival, we remain cautiously hopeful about its long-term prognosis.
There are additional reasons for guarded optimism about the future for A. cunemica sp. nov., in our judgement. Because the species lives in mid-elevation portions of the Northern Highlands, it probably has room to expand upward in elevation as the pine-oak forest is pushed upward by climate change [28, 30, but see 31]. This is unlike its close relative A. morenica , which appears to only live in high-elevation mountaintop cloud forest and is thus more strongly threatened by habitat loss driven by climate change [ 44]. Furthermore, although we encountered a few people who mistakenly feared A. cunemica sp. nov., believing it to be venomous, the overall lack of familiarity with the species by community members suggests that populations are not subject to serious pressure from unwarranted killing. Lastly, although arboreal members of the genus Abronia are increasingly targeted for the international illegal pet trade [ 103 – 105], we suspect that the extraordinary difficulty which we experienced in finding this species will reduce the intensity of this threat. Nonetheless, to further reduce any possible poaching pressure, we have intentionally masked the localities mentioned in this paper by (1) rounding GPS coordinates to the nearest hundredth of a degree, and (2) not reporting distance or direction from the nearest town [ 106].
Although all conclusions regarding threats are based on limited data and thus represent informed conjecture, we consider it justifiable to provisionally categorize A. cunemica sp. nov. on three lists of imperiled species. For the Red List of Threatened Species of the International Union for Conservation of Nature, we recommend that A. cunemica sp. nov be assessed as Endangered (B1ab[iii,v]+2ab[iii,v]) [ 107]. In meeting these criteria for Endangered status, we consider the species to have an extent of occurrence and area of occupancy of much less than 5,000 km 2 and 500 km 2, respectively; to be known from fewer than five locations; and to be experiencing a inferred decline in the extent of habitat, quality of habitat, and number of mature individuals, due to cutting of trees and possible loss of individuals to targeted killing and poaching [ 107]. Similarly, we calculate that A. cunemica sp. nov. has an Environmental Vulnerability Score (EVS, see Wilson et al. [ 108]) of 18 out of 20, which places the species in the High Vulnerability category. We derive this total score from assessed criteria on geographic distribution (6 points, for having a distribution limited to Mexico in the vicinity of the type locality), ecological distribution (8 points, for being currently known from only one vegetation formation), and degree of human persecution (4 points, for having arboreal habits yet thought to be harmful to people, and might be killed if encountered). Finally, we propose that A. cunemica sp. nov. be categorized as Amenazada (Threatened) on the Norma Oficial Mexicana list [ 109], due to the species meeting Criteria A.(I)+B.(II)+C.(II)+D.(III) as defined by the Anexo Normativo I or Método de Evaluación del Riesgo de Extinción de las Especies Silvestres en México (MER) [ 110]. This is the only list of at-risk species that is legally recognized by the Mexican government. We suggest this categorization based on A. cunemica sp. nov. having the following characteristics: a very restricted distribution (4 points) that encompasses less than 5% of the national territory of Mexico; an intermediate or limiting habitat (2 points) with respect to the requirements for the natural development of the species, due to its presumed restriction to mid-elevation forests; a medium vulnerability (2 points) intrinsic to the biology of the species, due to its specialized natural history that depends on trees but allows the species to persist in fragmented forests; and a medium impact (3 points) by humans on the species due to pressure from human settlements, moderate habitat fragmentation, and the potential use, trade and trafficking of the species. The total score of 11 points justifies an assignment to the category of Amenazada [ 109].
No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.