Colwellia mytili, Kim & Park & Park & Nam & Jung & Kim & Yoon, 2017

Kim, Young-Ok, Park, In-Suk, Park, Sooyeon, Nam, Bo-Hye, Jung, Yong-Taek, Kim, Dong-Gyun & Yoon, Jung-Hoon, 2017, Colwellia mytili sp. nov., isolated from mussel Mytilus edulis, International Journal of Systematic and Evolutionary Microbiology 67 (1), pp. 31-36 : 34-35

DESCRIPTION OF COLWELLIA MYTILI SP. NOV.

Colwellia mytili (my′ ti.li. L. gen. n. mytili of Mytilus , named after the generic name of the mussel Mytilus edulis , from which the type strain was isolated).

Cells are Gram-stain-negative, non-spore-forming, rodshaped, approximately 0.3–0.7 µm in diameter and 0.5–8.0 µm in length, and motile by means of a single polar flagellum. Colonies on MA are circular, slightly convex, glistening, smooth, greyish-yellow and 0.8–1.5 mm after incubation for 5 days at 20 Ǫ C. Optimal growth is observed at 20 Ǫ C; growth occurs at 4 Ǫ C and slowly at 25 Ǫ C, but not at 28 Ǫ C. Optimal pH for growth is between 7.0 and 8.0; growth occurs at pH 6.0, but not at pH 5.5. Growth occurs with 1.5–4.0 % (w/v) NaCl (optimum 2.0–3.0 %). Growth does not occur under anaerobic conditions on MA and on MA supplemented with nitrate. Catalase- and oxidase-positive. Nitrate is not reduced to nitrite. Casein, starch and Tween 80 are hydrolysed, but aesculin, gelatin, hypoxanthine, L- tyrosine, urea and xanthine are not. Pyruvate is utilized as carbon and energy source, but L- arabinose, cellobiose, D- fructose, D- galactose, D- glucose, maltose, D- mannose, sucrose, trehalose, D- xylose, acetate, benzoate, citrate, formate, L- malate, succinate, salicin and L-glutamate are not. In assays with the API ZYM system, activity of alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase and acid phosphatase is present and activity of naphthol-AS-BI-phosphohydrolase is weakly present, but activity of lipase (C14), valine arylamidase, cystine arylamidase, trypsin, a- chymotrypsin, a- galactosidase, b-galactosidase, b- glucuronidase, a- glucosidase, b- glucosidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase is absent. Susceptible to ampicillin, carbenicillin, cephalotin, chloramphenicol, gentamicin, novobiocin, oleandomycin, penicillin G, polymyxin B and streptomycin, but not to kanamycin, lincomycin, neomycin and tetracycline. The predominant ubiquinone is Q-8. The major fatty acids (>10 % of the total fatty acids) are summed feature 3 (C 16: 1 Ɯ7c and/or C 16: 1 Ɯ6c) and C 16: 0. The major polar lipids are phosphatidylglycerol and phosphatidylethanolamine.

The type strain, RA2-7 T (= KCTC 52417 T = NBRC 112381 T), was isolated a mussel ( Mytilus edulis ) collected from Cheongsan island in the South Sea, South Korea. The DNA G+C content of the type strain is 39.0±0.04 mol %.