Data, Walker, 1862

Afrand, Mahboobeh, Sourinejad, Iman, Fazeli, Seyed Abolhassan Shahzadeh, Akbarzadeh, Arash, Yeganeh, Laleh Parsa, Sadeghi, Maryam & Azarbaijani, Reza, 2020, Morphological identification and molecular validation of anchovies (Engraulidae) in the Persian Gulf and Oman Sea, Zootaxa 4742 (2), pp. 375-391 : 377

Data analysis

Forward and reverse sequences were assembled and manually edited using the SeqMan software (DNAStar). We added to our dataset the COI sequences of Chirocentrus dorab , Dussumieria acuta , Nematalosa nasus , Sardinella albella , and Sardinella gibbosa (non-engraulid Clupeoidei) and Denticeps clupeoides (Denticipoidei) as out group to root our phylogenetic tree. All sequences were aligned using CLUSTAL W ( Thompson et al. 1994) in Meg Align software (DNAStar) and variable sites were determined. Pairwise sequence divergence was calculated by using Kimura’s two-parameter (K2P) distance ( Kimura 1980). Maximum Likelihood (ML) trees with bootstrap analysis of 1000 replicates based on K2P distance were constructed in MEGA version 6.0 ( Tamura et al. 2013).