Sesamum indicum, cytochrome P

Deshmukh, Aaditi B., Datir, Sagar S., Bhonde, Yogesh, Kelkar, Natasha, Samdani, Pawan & Tamhane, Vaijayanti A., 2018, De novo root transcriptome of a medicinally important rare tree Oroxylum indicum for characterization of the flavonoid biosynthesis pathway, Phytochemistry 156, pp. 201-213 : 211

4.11. Phylogenetic analysis of O. indicum cytochrome P 450

Genes annotated as Cytochrome P 450 in the transcriptome were shortlisted (Supplementary Table 4 ). They were used to identify the full length ORFs of OinCyp450s with high FPKM values (above 5.5). Protein BLAST of the genes annotated as cytochrome P450 s was performed to identify the type /function of the specific Cytochrome P450 . Phylogenetic tree of the newly identified, full length protein OinCyps was performed using MEGA7 by the maximum likelihood method ( Kumar et al., 2016) ( Fig. 5 ) .

Conflicts of interest

The authors declare no conflict of interest.

Acknowledgement

The authors would like to acknowledge the Department of Biotechnology (DBT), Government of India for the DBT-BioCARE project to Dr. Vaijayanti Tamhane (BT/Bio-CARe/07/320/2012) and Departmental Research and Developmental Program (DRDP), Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University (SPPU), Pune, Maharashtra, India for funding this work. The authors are thankful to Ms. Meenakshi Kapse, Project Assistant, IBB, SPPU for helping with command line BLAST analysis of the transcriptome. The authors are grateful to Dr. Sneha Bansode, Department of Biotechnology, SPPU for her critical comments and suggestions. The transcriptome sequencing service was provided by Genotypic, Bangalore, India.

Figures

Fig. 5. Phylogenetic analysis of full length O. indicum Cytochrome P450 using maximum likelihood method. Phylogenetic tree of the newly identified, full length OinCyps (Tables 7 and 8) was performed using MEGA7 by maximum likelihood method.