Forcipomyia parasecuris, Paul, Nilotpol, Harsha, Rupa & Mazumdar, Abhijit, 2014

Paul, Nilotpol, Harsha, Rupa & Mazumdar, Abhijit, 2014, A new species of Forcipomyia Meigen (Diptera: Ceratopogonidae) described with immature stages from India, Zootaxa 3881 (2), pp. 165-174 : 171-173

Forcipomyia parasecuris sp. n. ( Figs. 1 –27)

Type Material. Holotype female adult with associated larva and pupa (reared), Burdwan, West Bengal (Lat. 23º14’ N, Long. 87º50’E), 09.VIII.2012, leg. R. Harsha. Paratypes 3 females and 2 males with larvae and pupae (reared), data same as Holotype. 1 female and 3 males with associated larva and pupae (reared), Bhadreswar, West Bengal (Lat. 22º49’ N, Long. 88º19’E), 14.IX.2013, leg. N. Paul.

Etymology. The species has been named after Forcipomyia securis Chan & LeRoux , for its immense similarity with that species.

Diagnosis. Female adult may be separated from other female Forcipomyia sp. by flagellomeres 1–8 longer than flagellomeres 9–12; 2 basal palpal segments attached; 4–5 small sub apical setae arranged like comb in fore tibia; presence of long empodium with many setae; faint R1, second radial cell indistinct and covered with yellow setae; subgenal plate ring shaped.

Male adult may be separated from other male Forcipomyia sp. by posterior light sclerotized (pale yellow) and basally sclerotized (dark brown) aedeagus.

Pupa was separated from other pupa of Forcipomyia sp. by absent of ocular sensilla, presence of fine spicules on surface of 1to 4 abdominal segments and rest of segment surface smooth. Larvae separated by lack of definite eyespot and surrounding pit.

Female. (n = 5) Figs. 1–7

Total length 1.65–1.77 mm

Head ( Figs. 1–2 ). Brown, eyes with only a narrow line separation over their whole width in front and dorsomental extension 140–157 Μm long, medially 4 ommatidia in width. Interocular space with uniserial, ‘V’ shaped 10–13 strong setae. Antennae ( Fig. 1 ) with 13 flagellomeres (except pedicel) with pale brown colour, flagellomeres 1–8 equal in size with 37–42 Μm long, flagellomeres 9–12 even darker with 32–35 Μm long; 13th flagellomere 60–70 Μm long with slightly constricted apical nipple. Sensilla in flagellomeres: 1–8 flagellomeres possess 8–9 Μm long bristle like sensilla chaetica arrange basally in ring and 2 long sensilla trichodea, 9–13 flagellomeres possess 4–5 short bristle like sensilla chaetica arrange basally in ring and 3–4 short sensilla trichodea. AR 0.60–0.63.

Palpus dark brown, 3rd segment elongated and swollen basally with a bare pit opening on it. 2 basal palpal segments intimately attached. Length of palpal segments ( Fig. 2 ) 1–5: 22–25 Μm, 32–36 Μm, 76–81 Μm, 29–31 Μm, 30–33 Μm. PR 2.58–2.64. Clypeus brown and triangular with 8–10 setae.

Thorax. Light brown to deep brown in colour with few anterior hairs, prealars with small spiniform setae and scutum with simple seta; scutellum brown black with 8–9 large setae serially arranged and 10–12 small setae; post scutellum bare and short, deep black in margin.

Leg ( Figs. 3–5 ). Femur and tibia of all legs yellow except hind leg femur apically black. Fore leg: tibia with 180–190 Μm long numerous setae scattered along the length, 4–5 small sub apical setae arranged like comb along with 38–42 Μm long spine ( Fig. 3 ) in tibia, ventral palisade setae in one row on tarsomeres 1and 2, TR 0.98–1.00. Mid leg: 2 strong setae each on tarsomeres 1 to 4, TR 0.78–0.88. Hind leg: tibia with 250–270 Μm long numerous setae scattered along length, tibial comb ( Fig. 4 ) biserial, 9 setae on lower serial with one spur and 12–14 setae on upper serial, TR 0.72–0.77. Claws ( Fig. 5 ) equal, curved, moderately stout; all legs bear a 32–36 Μm long empodium with course of seate. Lengths of leg segments in table 1.

Femora Tibia Tarsomeres

1 2 3 4 5 Fore 328–337 334–340 105–109 97–105 60–64 57–60 50–55 Mid 405–412 430–412 97–105 119–135 84–91 60–67 59–65 Hind 429–435 415–420 115–123 158–165 90–93 62–67 60–62 Wing ( Fig. 6 ). Wing length 0.81–0.83 mm and breadth 360–375 Μm, Costal ratio 0.47–0.50.

Wing membrane covered with dense macrotrichia. First radial cell and R2 obliterated, R1 faint, second radial cell indistinct, and covered with yellow setae, proximal area of beyond R3 with dense black. Fork of cubitus situated beyond costa. Halter large and dark black with 5–6 strong setae.

Abdomen ( Fig. 7 ). Tergites 1–7 uniformly dark brown. Two well-developed ovoid spermathecae ( Fig. 7 ) present on tergite 7, large one 66–74 Μm long and 53–66 Μm wide with well develop neck whereas smaller one 59–62 Μm long and 45–53 Μm wide without neck. Sternite 8 anteriorly dark, posteriorly hyaline and weakly sclerotized; anterolateral margin of sternite 8 pointed and posteromedial portion broad somewhat lobe like. Sternite 9 outwardly pointed and pale yellowish. Cerci pale brown.

Male. (n = 5) Fig. 8

Similar to female with usual sexual differences: Total length 1.85–1.90 mm. Flagellomeres 1–9 conical and pale, flagellomere 10 longest, flagellomeres 11–13 brown and elongated; AR 0.78–0.82. Wing 0.88–0.90 mm long, 290–310 Μm wide, CR 0.40–0.42; PR 3.50–3.70. Wing with less microtrichia and R1 quite visible, subcosta extend up to R2.

Genitalia ( Fig. 8 ): Tergite 9 short 62–70 Μm long, slight concave posterior margin with rounded distally. Gonocoxite 131–140 Μm long, 2× longer than greatest breadth; gonostylus 90–97 Μm long, straight with slightly bent blunt tip. Parameres separated, basal arm slender 35–39 Μm long and posterior process 131–139 Μm long with seta like apical portion. Aedeagus roughly triangular, breadth 90–95 Μm basally; basal arm outwardly curved, mid portion excavated and highly sclerotized dark and brown in colour; posterior portion stout, blunt and light sclerotize and pale yellow in colour.

Pupa. (n = 4) Figs. 9 –19

Total length ( Fig. 9 ) 1.950–2.281 mm. Coloration of exuviae pale to dark yellow, respiratory organ golden brown. Dorsal apotome roughly triangular comprise with equal distributed sharp spicule; dise 0.58–0.65 × longer than greatest width and posterior margin with smooth apex, dorsal subapical tubercle (t) absent. Cephaothorax ( Fig. 10 ) roughly oval, 885–1020 Μm long, 660–750 Μm wide, dorsal surface with minute spicules randomly distributed and ventrally smooth. Ceplalothorax sensilla: D-1-T, D-2-T and D-4-T serrated and formed long process. D-1-T ( Fig. 11 ) 187–210 Μm long with apically stout setae, D-2-T ( Fig. 12 ) 165–188 Μm long, D-4-T 121–140 Μm long and D-3-T on rounded large tubercle; SA-2-T on small tubercle and more posteriorly placed. Respiratory organ ( Fig. 13 ) nearly straight, deep brown, bulbous apically and a central depressed area, 15–17 apico-lateral pores present and a large pore with 2–3 spiracles placed mid-laterally; RO 150–175 Μm long and 56–62 Μm wide. Pedicel smooth and short 20–24 Μm long, P/RO 0.12–0.13. Only dorsolateral cephalic sclerite sensilla DL-1-H present, short, stout and spine like. CL-1-H absent, CL-2-H ( Fig. 14 ) 19–23 Μm long, stout chaetica setae. Ocular sensillum absent ( Fig. 14–14 a). First abdominal segment setae ( Fig. 15 ): D- 2-I minute on rounded small tubercle; D- 8-I 31 –35 Μm long, robust and spine like on a large tubercle postero- medially placed; D- 4-I pore without tubercle; D- 7-I pore anteromedial to D- 8-I; L- 2-I stout, 32–36 Μm long arise on a tubercle.

Fourth abdominal segments setae ( Figs. 16 –17): D- 2-IV on large serrated tubercle; D- 3-IV thin, 26–29 Μm long; D- 4-IV absent; D- 5-IV on stout tubercle with 28–32 Μm long; D- 7-IV and D- 8-IV absent. L- 1-IV and L- 2-IV both stout and 18–22 Μm long; L- 3-IV and L- 4-IV both closely placed, 11–14 Μm long. V- 7-IV thin, 15–18 Μm long on tubercle, V- 6-IV thin and wide apart from V-7 -IV. Spiracle absent. Second to seven abdominal setal patterns similar to fourth segment, except length and position. Surface of segments 1to 4 with fine spicules, rest surface of segments smooth.

Segment 9 (Figs. 18–19): 387–415 Μm long and 185–210 Μm wide, 1.90–2.15 × longer than width; surface of the segment generally smooth, a group of small spicules placed mid-lateral on both side. Terminal portion 215–247 Μm long, slightly diverge without any outer setae.

Fourth instar Larvae. (n = 5) Figs. 20 –27

Total length ( Fig. 20–20a ) 2.84–3.50 mm.

Living larvae brown with black shaded, exuviae yellow.

Head capsule ( Fig. 21–21 a). Golden brown, opisthognathous and well developed. HL 295–375 Μm, HW 195–260 Μm, HR 1.42–1.46. Head chaetotaxy as Follows: ‘p’ 105–123 Μm long, lanceolate; ‘q’ 110–127 Μm long, lanceolate; ‘s’ 37–43 Μm long, spinose; ‘t’ 58–65 Μm long spinose; ‘o’ 74–77 Μm long hair like; ‘z’ 35–40 Μm long; ‘y’ 60–65 Μm long with large pore; ‘v’ 95–100 Μm long and spine like. Antennae 2 segmented, basal half 81–86 Μm long and apical one 4–5 Μm long, arise from basal tubercle. Definite eyespots and surrounding pit absent.

Labrum (LB) 42–48 Μm long, equal with hypostoma; palatum (PL) 22–26 Μm log, abutting to messors (MS) with one sensilla styloconica (SS) and one sensilla trichoidea (ST). Messors bifurcated and pointed, MS to PL 70–76 Μm long. Maxilla ( Figs. 22–23 ) shield shaped triangular, outer margin fringe 45–48 long, basally 12–15 Μm wide with centrally large maxillary palpus and a sensilla basiconica (SB). Mandible ( Figs. 22–23 ) short and stout with proximal end light bent of fossa mandibularis; ML 35–40 Μm and MW 17–24 Μm. Galeolacinia ( Fig. 23 ) with two branch lacinial sclerite 1 (LC1) flat and lacinial sclerite 2 (LC2) lobule shaped, 10 Μm long, closely attached with basal maxilla. Hypostoma ( Fig. 22 ) 45–50 Μm long flattened and basally arched. Epipharynx 80–87 Μm long, gently massive and arched.

Thorax: Each ramus of prothoracic pseudopod contains seven equal strong black ( Fig. 24 ), backwardly directed hooklet.

Abdomen. Second to eight abdominal segment ( Figs. 20–20a ) possess same chaetotaxy. Second segment (Figs. 25–25a): ‘a’ 105–115 Μm long, lancelet setae; ‘b’130–137 Μm long, deep brown and serrated on one side; ‘c’ 92–97 Μm long, stout and apically trifurcated; ‘d’ 74–78 Μm long, pale yellow and simple; ‘e’ 75–77 Μm long and simple; ‘f’ 53–78 Μm long and simple; ‘g’ longest setae, 160–188 Μm long. A deep brown band present between two ‘a’ seta of each segment. 4–7 segment bearing very long ‘d’ and ‘g’ seta. Ninth to twelve segments (Figs. 26) without ‘e’ and ‘f’ setae. Anal pseudopod serrated laterally with two rows of 7 recurved hooklets directed posteriorly; anal blood gill (Fig. 27) 4 segmented bearing outer two 70–80 Μm long stout setae, ASL 160–185 Μm, ASW 130–145 Μm.

Figures

FIGURES 1 – 8. 1 – 7. Forcipomyia (F.) parasecuris sp. n., adult female. 1: antenna, 2: palpomere, 3: apex of fore tibia, 4: apex of hind tibia, 5: claw and empodium, 6: wing, 7: female genitalia with spermatheca. Scale bar 76 µm. 8. Forcipomyia (F.) parasecuris sp. n., adult male. 8: male genitalia. Scale bar 76 µm.

FIGURES 9 – 13, 17 – 19. Forcipomyia (F.) parasecuris sp. n., pupa. 9: pupa dorsally, 10: cephalothorax dorsally, 11: Ceplalothorax sensilla D- 1 - T, 12: Ceplalothorax sensilla D- 2 - T, 13: respiratory organ, 17: forth abdominal segment, 18: segment 9 (male), 19: segment 9 (female). Dorsal sensilla (D – 1 – T, D – 2 – T, D – 3 – T, D – 4 – T); dorsal sensilla of segment four (D – 2 – IV, D – 3 – IV, D – 5 – IV); dorsolateral cephalic sclerite sensilla (DL- 1 - H); GL, genital lobe; lateral sensilla of segment four (L – 2 – IV); supralar (SA – 2 – T); TP, terminal process.

FIGURES 14 – 16. Forcipomyia (F.) parasecuris sp. n., pupa. 14: palpus and clypeal / labral sensillum, 14 a: photograph of clypeal / labral sensillum and ocular area, 15: first abdominal segment, 16: fourth abdominal segment. Clypeal / labral sensilla (CL – 2 – H); dorsal sensilla of first abdominal segment (D – 2 – I, D – 4 – I, D – 7 – I, D – 8 – I); dorsal sensilla of segment four (D – 2 – IV, D – 3 – IV, D – 5 – IV); lateral sensilla of first abdominal segment (L – 2 – I); lateral sensilla of segment four (L – 1 – IV, L – 2 – IV, L – 3 – IV, L – 4 – IV); ventral sensilla (V – 6 – IV, V – 7 – IV). Scale bar 76 µm.

FIGURES 20 – 24 & 27. Forcipomyia (F.) parasecuris sp. n., larva. 20: larva, 21: head capsule with cephalic setae and sensory pore, 22: mouthparts of larva, 23: close view of mouthparts, 24: claw of anterior parapod, 27: caudal end of larva. Head capsule chaetotaxy: o, parahypostomal setae; p, posterior perifrontal; q, postfrontal setae; s, anterior perifrontal setae; t, prefrontal setae; v, posterolateral setae; y, ventral setae; z, frontal pit seta. AN, antennae; GL, genital lobe; H, hook; HY, hypostoma; LB, labrum; LC 1, lacinial sclerite 1; LC 2, lacinial sclerite 2; MD, mandible; MX, maxilla; MS, messors; PL, palatum; SS, sensilla styloconica; ST, sensilla trichoidea.