Tricharina tophiseda Matočec & I. Kušan, 2015

Kušan, Ivana, Matočec, Neven, Mešić, Armin & Tkalčec, Zdenko, 2015, Tricharina tophiseda-a new species from Croatia, with a revision of T. japonica (Pyronemataceae, Pezizales), Phytotaxa 221 (1), pp. 35-47 : 38

Tricharina tophiseda Matočec & I. Kušan , sp. nov., Fig. 1 , 2 , 3A–L

MycoBank MB 811923

Type: — CROATIA. Dalmatia: 3500 m E-SE from Skradin, right bank of Krka river in the area of Skradinski buk waterfalls, elev. 13 m, 43°48’22’’ N, 15°57’51’’ E, habitat: tufa barrier surrounded by Populus sp. , Fraxinus angustifolia , Platanus sp. and Ficus carica riparian vegetation, substratum: continuously wet tuffaceous sediment covered with mosses and scarcely covered with minute plant remnants, together with Trichophaea woolhopeia s. l., 30 November 2008, N. Matočec & I. Kušan, (holotype CNF 2/8079).

Etymology: —Specific epithet tophiseda (tophus = tufa, sedere = sitting) means “sitting” on a tuffaceous substrate.

Figures

FIGURE 1. Tricharina tophiseda.A.Ascus apex with the ascospore.B.Ascus apex. C. Paraphyses.D.Ascus base with a part of ascogenous system. E–F. Ascospores (representing all shape variations). G. Marginal hairs apices and a middle part. H. Marginal hair fascicle. I. Excipular texture. A–E, G, I from (CNF 2/8079, holotype), C, F, H from (CNF 2/7953). All in *H O, except B is in *CR. Scale bars: A–F 2 = 10 μm, G, I = 50 μm, H = 100 μm. Del. N. Matočec.

FIGURE 2. Tricharina tophiseda. A–D.Living apothecia.E. Section through the apothecia. F. Marginal and submarginal area (side view). G. Margin. H. Paraphyses and ascospores (two lower ascosp. in early stage of germination). I. Asci with ascospores. J–K. Ascospores. L. Asci (each in a different focal view). M. Middle part of the marginal hair. N. Ascospores (with visible BSG). O–R. Cells in a meddulary excipulum.A–C, E–R from (CNF 2/8079, holotype), D from (CNF 2/7953). E–J, O in *H O, K in †KOH, L in *CR, M, R in *CRB-KOH, P 2 in *IKI. Scale bars:A = 5 cm, B–C = 2 cm, E–G = 200 μm, M, O–R = 20 μm, H–L, N = 10 μm. Phot. I. Kušan & N. Matočec.

FIGURE 3. Tricharina tophiseda—Colony and ascorhizoctonia-type anamorphic structures on CYA (A–J) and MEA (K–L). A. Germinating ascospores. B. Colony after 1 day. C. Colony rim after 2 days. D. Mycelial branching with the septal positions. E. Terminal and lateral nodules. F. Mycelial loops.G. Smooth and rough walled hyphae. H.Young moniliform sporodochia-like cell aggregates. I. Ripe moniliform sporodochia-like cell aggregates. J. Aerial hyphae (after 5.5 days). K. Hyphae preceding to formation of sporodochia-like cell aggregates. L. Moniliform sporodochia-like cell aggregates. T. japonica—Apothecia, ascospores and excipular structures (M–W). M. Part of the type collection. N. Section from the margin to the apothecial base (red arrow). O. Marginal hairs. P. LBs in the ectal excipular cells. R. Excipular hairs (side view). S. Excipular hairs with bulbous base (section). T–U.Ascospores.V. Subicular hyphae.W.Ascospores.A–L from (CNF 2/8079, holotype), M–O, R, T–V from (CUP-K-(JA-000286), holotype), P, S, W from (R.D. 31.01.245.11). D–K, W in *H O, 2 N–O, T, V in †KOH, P–S, U in †CB. Scale bars: B–C, M = 5 mm, N = 200 μm, J, O, R = 100 μm, S, V = 50 μm, A, H–I, P = 20 μm, D–G, K–L, T–U, W = 10 μm. Phot. I. Kušan & N. Matočec.