Nigrospora solani Silva, R. M. F

Silva, Rejane M. F., De Mélo, Mayara A. C., Oliveira, Thays G. L., De Oliveira, Rafael J. V., Souza-Motta, Cristina M. & Silva, Gladstone A., 2024, Nigrospora solani (Amphisphaeriales, Apiosporaceae) a new endophytic species from Brazil, Phytotaxa 669 (3), pp. 273-284 : 279

Nigrospora solani Silva, R. M. F ; Correia de Mélo, M. A. & Silva, G. A., sp. nov. ( Fig. 2 )

MycoBank no.:—MB845957

Etymology:—Named in reference to the host genus Solanum in which the fungus was collected.

Habitat:—Healthy leaves of Solanum lycopersicum var. cerasiforme .

Distribution:—Organic farming system in Pernambuco, Brazil.

Description:—The mycelial hyphae are smooth, hyaline, branched, septate, and 4–5 μm in diam. The conidiophores are reduced to conidiogenous cells. The conidiogenous cells are monoblastic, discrete, smooth, solitary, initially hyaline becoming pale brown with age, and globose, subglobose, or ampulliform (5–6 × 5 μm). The conidia are solitary, globose to subglobose, spherical, black, smooth-walled, shiny and aseptate (12–14 × 15 μm).

Culture characteristics:—On PDA, forms flat colonies, floccose, olivaceous surface, dark brown on reverse, and reaches 8 cm in diam. in 14 d at ±28 °C. On SNA, forms flat colonies, dark brown surface with black patches, dark brown reverse with black patches, and mycelium with slow sporulation reaching 3 cm in diam. in 14 d at ±28 °C.

Holotypus:— Brazil, Pernambuco, Lagoa de Itaenga (7°53’42.9”S, 35°16’52.5”W), Jan 2021, leg. M. A. Correia de Mélo (Holotype HURM 94667 ; ex-type URM 8459 = RV01 ; ibid. RV02 ) .

Notes — Nigrospora solani form a single well-supported clade close to N. endophytica , N. macarangae , and N. pernambucoensis . Morphologically, N.solani differs from N.endophytica by the presence of ampulliform conidiogenous cells that are smaller (5–6 × 5 μm vs. 6.2–10 μm) ( Brito et al. 2023). Nigrospora solani can be distinguished from N. macarangae by the absence of conidiogenous cells aggregated in clusters and the presence of smaller conidiogenous cells (5–6 × 5 μm vs. 7–10 × 11–14 μm) ( Tennakoon et al. 2021). Nigrospora pernambucoensis produces conidiogenous cells that are pale brown to dark brown, globose to obpyriform and has hyaline vesicles around the septum delimiting the conidia and their conidiogenous cells ( Brito et al. 2023). In contrast, N. solani produces conidiogenous cells that are subglobose, or ampulliform, initially hyaline and become pale brown with age, vesicles around the septum delimiting the conidia and their conidiogenous cells are absent in N. solani .

Figures

FIGURE 2. Morphology of N. solani sp. nov.A. Colony morphology on potato dextrose agar (PDA) after 15 d. B. Colony morphology on synthetic nutrient-poor agar (SNA) after 15 d. C, D, E, and F. Conidiogenous cells and conidia. Scale bars = 10 µm.