Paracrassiperidium fusiforme Jayasiri, Tibpromma & Karun., 2024

Jayasiri, Subashini Chathumini, Tibpromma, Saowaluck, Xu, Ruifang, Bhat, Darbhe Jayarama, Elgorban, Abdallah M., Karunarathna, Samantha C., Rampelotto, Pabulo H., Han, Meiyan & Xu, Jianchu, 2024, Unveiling new fungal discoveries in Yunnan Province, China, Phytotaxa 676 (2), pp. 103-125 : 115

Paracrassiperidium fusiforme Jayasiri, Tibpromma & Karun. , sp. nov. FIGURE 6

Index Fungorum number: IF903020

Etymology: The epithet “fusiforme ” refers to the spore shape of the new taxon.

Holotype: HKAS 115543

Saprobic on dead branches of Taxodium mucronatum . Sexual morph: Ascomata 250–300 × 200–235 µm (x̄ = 270 × 220 µm, n = 10), visible as black dots, small dome-shaped on host surface, immersed to semi-immersed, scattered, subglobose, uni-loculate, with a papillate ostiole. Peridium 25–40 µm (x̄ = 30 µm, n = 20), of unequal thickness, composed of several layers of brown to dark brown cells, arranged in textura angularis. Hamathecium composed of dense, 1.1–1.5 µm wide, filamentous, branched, aseptate, pseudoparaphyses, anastomosed between asci, embedded in a hyaline gelatinous matrix. Asci 70–150 × 9–13 µm (x̄ = 110 × 10.7 µm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, pedicellate, and with well-developed ocular chamber. Ascospores 20–35 × 2.6–4.3 µm (x̄ = 28 × 3.8 µm, n = 20), overlapping 1–2-seriate, fusiform, hyaline to light brown, 1–5 septate, constricted at the septa, the upper cell larger than the lower cell, conical at the ends, guttulate, smooth-walled, with mucilaginous sheath. Asexual morph: undetermined.

Culture characteristics: Ascospores germinated within 12 hours on PDA; colonies were grown on PDA at room temperature (20–25 °C). Colonies circular to irregular, concentric surface, grey to dark brown, aerial hyphae. Dark brown to black on the reverse.

Material examined: China, Yunnan Province, Kunming, Kunming Institute of Botany Botanical Garden, Chinese Academy of Science, on dead branches of Taxodium mucronatum ( Cupressaceae ), 08 May 2020, Tibpromma S, ST 20 (HKAS 115543, holotype), ex-type KUMCC 21-0041a, KUMCC 21-0041b.

Notes: Paracrassiperidium fusiforme is morphologically similar to the members of Pleosporales in having perithecioid ascomata, bitunicate asci, and hyaline to brown ascospores. Paracrassiperidium fusiforme (KUMCC 21-0041a and KUMCC 21-0041b) formed a distinct lineage sister to other genera in Longiostiolaceae ( Figure. 5 ). Paracrassiperidium has 8-spored asci, hyaline to light brown, fusiform ascospores with 1–5-septa, Crassiperidium has 4–8-spored asci, hyaline, broadly fusiform ascospores with 1–3-septa ( Matsumura et al. 2018), and Longiostiolum has ascostromata with a long central ostiole with phragmosporous ascospores ( Li et al. 2016).

Figures

FIGURE 5. The phylogram generated from maximum likelihood analysis based on LSU, SSU, TEF1, and RPB2 sequence data represents Longiostiolaceae and closely related families. Related sequences are obtained following Wanasinghe et al. (2020). Thirty-six strains are included in the combined analyses, with three species from the family Melanommataceae being used as the outgroup taxa. Bootstrap values for ML are equal to or greater than 70%, and clade credibility values equal to or greater than 0.95 (the rounding of values to 2 decimal proportions) from the BYPP analysis labeled on the nodes.A newly generated sequence is in blue, and type species are bold.

FIGURE 6. Paracrassiperidium fusiforme (HKAS 115543, holotype). a, b Ascomata on host surface. c Vertical section of ascoma. d Peridium. e–h Asci. i–m Ascospores (arrow shows mucilaginous sheath). n Germinated ascospore. o Upper view on PDA. p Reverse view on PDA. Scale bars: a–b = 1000 μm; c = 100 μm; d = 30 μm; e = 20 μm; f–h = 30 μm; i–n = 10 μm.