Neopestalotiopsis, S.S.N.Maharachchikumbura, K.D.Hyde & P.W.Crous, 2014

Huanaluek, Naruemon, Jayawardena, Ruvishika S., Maharachchikum- Bura, Sajeewa S. N. & Harishchandra, Dulanjalee L., 2021, Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov., Phytotaxa 479 (1), pp. 23-43 : 32

Neopestalotiopsis

MP, ML and BYPP analyses produced nearly identical topologies. Fifty-nine strains are included in the analyses, which comprised 1,455 characters including gaps. The tree was rooted with Pestalotiopsis diversiseta (MFLUCC 12–0287). The maximum parsimonious dataset consisted of 1,037 constant, 216 parsimony-informative and 202 parsimonyuninformative characters. The parsimony analysis of the data matrix resulted in the maximum of ten equally most parsimonious trees with a length of 808 steps (CI = 0.644, RI = 0.647, RC = 0.416, HI = 0.356) in the third tree. The best scoring RAxML tree with a final likelihood value of -6463.200637 is presented. The matrix had 488 distinct alignment patterns, with 8.33% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.228199, C = 0.269018, G = 0.213127, T = 0.289656; substitution rates AC = 1.235844, AG = 3.930484, AT = 1.813207, CG = 0.912315, CT = 5.091895, GT = 1.000000; gamma distribution shape parameter α = 0.762342. In our analyses ten isolates formed a distinct clade with high statistical support and neighbouring to N. musae (MFLUCC 15–0776) and N. alpapicalis (MFLUCC 17–2544 and 17–2545) ( Fig. 1 ). Therefore, the new lineage is introduced here as the new species Neopestalotiopsis hydeana .

Figures

FIGURE 1. Consensus phylogram of 1,000 trees resulting from a RAxML analysis of the (ITS+TUB2+ Tef1) alignment of the analysed Neopestalotiopsis sequences. RAxML bootstrap support values (ML above 60%), maximum parsimony (MP above 60%) and Bayesian inference (BYPP above 0.90) are given at the nodes (ML/MP/BYPP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree is rooted to Pestalotiopsis diversiseta MFLUCC 12–0287.