Salinadaptatus halalkaliphilus, Xue & Zuo & Zhou & Zhou & Zhang & Han & Zhao & Xiang, 2021

Xue, Qiong, Zuo, Zhenqiang, Zhou, Heng, Zhou, Jian, Zhang, Shengjie, Han, Jing, Zhao, Dahe & Xiang, Hua, 2021, Salinadaptatus halalkaliphilus gen. nov., sp. nov., a haloalkaliphilic archaeon isolated from salt pond in Inner Mongolia Autonomous Region, China, International Journal of Systematic and Evolutionary Microbiology (004584) 71 (1), pp. 1-10 : 9

DESCRIPTION OF SALINADAPTATUS HALALKALIPHILUS SP. NOV.

Salinadaptatus halalkaliphilus (hal.al.ka.li′ phi.lus. Gr. masc. n. hals halos salt; N.L. n. alkali alkali; Gr. masc. adj. philus friend, loving; N.L. masc. adj. halalkaliphilus salt and alkali-loving). Cells are motile, rod-shaped and Gram-stain-negative. Cell size is 0.4–0.8µm wide and 1.0–2.0µm long. Colonies are pink, smooth, convex, round, and 1.0–2.0 mm in diameter when cultured on the AM plate at 42 °C for 7–10days. Cell lysis occurs in distilled water and the minimal NaCl concentration to prevent cell lysis is 0.85 M. Growth occurs in the presence of 2.6–5.3 M NaCl (optimum 4.4 M), at 30–50 °C (optimum 42 °C) and pH 7.0–10.0 (optimum pH 8.0–8.5). No requirement for Mg 2+, but grows well with the addition of Mg 2+ up to 5 mM. Oxidase and catalase tests are positive. Anaerobic growth does not occur in the presence of nitrate, L-arginine or DMSO. H 2 S is produced from cysteine but not from thiosulfate. Indole is produced from tryptophan. Nitrate and nitrite cannot be reduced, and no gas formation occurs. Gelatin and Tweens 20, 40, 60, 80 are hydrolysed but starch and casein are not hydrolysed. The following substrates are utilized as single carbon and energy sources for growth: glycerol, D-glucose,D-galactose, sucrose, lactose, D-sorbitol, acetate, succinate, L-malate, fumarate, L-glutamate, citrate, L-aspartate. The following substrates are not utilized as sole sources of carbon and energy: chitin, mannose, maltose, starch, pyruvate, lactate, L-alanine,L-ornithine,D-fructose, D-sorbose,D-xylose, glycine,L-arginine,L-lysine. Acid was produced from glycerol, but not fromD-glucose,D-galactose, sucrose, lactose, D-sorbitol, mannose, maltose, D-fructose, D-sorbose, andD-xylose. The major polar lipids are phosphatidylglycerol (PG) and phosphatidylglycerol phosphate methyl ester (PGP-Me), but glycolipids were not detected. The main quinones are MK-8 and MK-6 . The type strain, XQ-INN 246 T (=CGMCC 1.16692 T =JCM 33751 T), was isolated from a salt pond named Yanpaozi Lake of Xilingol , in Inner Mongolia Autonomous Region, China. The genomic DNA G+C content of this strain is 62.06 mol% (determined from the genome sequence).

The GenBank accession number of the 16S rRNA gene sequence of the strain XQ-INN 246 T is MK226479 .

The GenBank accession number of the genome sequence of the strain XQ-INN 246 T is RBZW00000000.