Melaminivora jejuensis, Kim & Park & Lee & Song & Kim, 2018

Kim, Ji-Young, Park, So-Hyun, Lee, Dong-Heon, Song, Gwanpil & Kim, Young-Ju, 2018, Melaminivora jejuensis sp. nov., isolated from Swinery waste, International Journal of Systematic and Evolutionary Microbiology 68 (1), pp. 9-13 : 11-12

DESCRIPTION OF MELAMINIVORA JEJUENSIS SP. NOV.

Melaminivora jejuensis (je.ju.en′ sis. N.L. fem. adj. jejuensis pertaining to Jeju, Republic of Korea, from where the type strain was isolated).

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Denitrifying, 0.5–0.7 µm (width)×2.0–3.5 µm (length), as assessed in 3 day-old cultures grown at 30 Ǫ C on TSA. Optimum growth is observed at 30–37 Ǫ C, at pH 7–8 and in the presence of 0–1 % NaCl (w/v). Hydrolyses gelatin and Tweens 20, 40, and 80, but does not hydrolyse casein, urea, Tween 60, tyrosine, aesculin and starch. Utilizes N -acetyl galatosamine, N -acetyl glucosamine, N -acetyl mannosamine, D- arabitol, adipate, glucuronamide, gluconate, myo - inositol, lactulose, malate, maltose, D- mannose, palatinose, raffinose, D- sorbitol, sucrose, bromosuccinic acid, b-hydroxybutyric acid, malic acid and succinamic acid. Produces alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase and naphthol-AS-BI-phosphohydrolase, but does not produce lipase (C14), trypsin, a- chymotrypsin, a- galactosidase, b- galactosidase, b- glucuronidase, a-glucosidase, b-glucosidase, N -acetyl-b- glucosaminidase, a- mannosidase, a- fucosidase, arginine dihydrolase, lysine decarboxylase or ornithine decarboxylase. Susceptible to ampicillin, cephalothin, chloramphenicol, gentamicin, kanamycin, neomycin, penicillin G, polymyxinB and tetracycline. The major fatty acids are (iso-C 15: 0 2-OH and/or C 16: 1 Ɯ 7 c), C 16: 0 and C Ɯ 7 c. The polar lipids are phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified lipid.

The type strain, KBB12 T (=KCTC 32230 T =JCM 18740 T), was isolated from swinery sludge in Jeju, Republic of Korea. The DNA G+C content of the type strain is 69.6 mol% .