Pseudomonodictys Doilom, Ariyaw., Bhat & K.D. Hyde

Bao, Dan-Feng, Hongsanan, Sinang, Hyde, Kevin D., Luo, Zong-Long & Nalumpang, Sarunya, 2022, Pseudomonodictys aquatica sp. nov., the sexual morph of Pseudomonodictys from freshwater habitats, Phytotaxa 567 (3), pp. 222-232 : 226-227

Pseudomonodictys Doilom, Ariyaw., Bhat & K.D. Hyde , Fungal Diversity 75, 88 (2015)

Saprobic on dead wood in freshwater or terrestrial habitats. Sexual morph: Ascomata solitary, scattered, semi-immersed to erumpent, visible as raised, globose to subglobose, black, ostiolate. Ostiole short papillate, with an irregular porelike opening, brown to dark brown. Peridium composed of several layers of brown to dark brown cells of textura agularis, merged with the host tissues. Hamathecium composed of dense, filamentous, branched, septate, hyaline, cellular pseudoparaphyses. Asci 8-spored, bitunicate, fissitunicate, clavate, short pedicellate, apically rounded, with an ocular chamber. Ascospores overlapping, 2–3-seriate, fusiform to cylindrical with round ends, slightly curved, multi septate, strongly constricted at the septa, narrow towards the both ends, enlarged at the middle cells, smooth-walled, hyaline, with small guttules, surrounded by a distinct, thick, mucilaginous sheath. Asexual morph: See Ariyawansa et al (2015).

Notes: Pseudomonodictys was known only from its asexual morph and the characteristics of sexual morph were unknown. In this study, we reported a sexual species Pseudomonodictys aquatica based on phylogenetic evidences. Pseudomonodictys clustered with Aquastroma , Lonicericola , Multilocularia, Paramonodictys and Paratrimmatostroma in Parabambusicolaceae ( FIGURE 1 ). Morphologically, Pseudomonodictys is similar to the sexual genera Aquastroma , Lonicericola and Multilocularia in having clavate asci with an ocular chamber and fusiform to vermiform, multiseptate ascospores with an entire sheath. However, Pseudomonodictys can be distinguished from Aquastroma and Multilocularia by the ascospores. Ascospores of Pseudomonodictys are 2–3-seriate, fusiform to cylindrical with round ends, strongly constrict at the septa and enlarged at the middle cells. While, ascospores of Aquastroma are clavate to fusiform, with a supramedian primary septum, slightly constricted at septa ( Tanaka et al. 2015) and Multilocularia has 1–2-seriate, ellipsoidal ascospores which are slightly constricted at the central septum ( Li et al. 2016). Pseudomonodictys is different from the asexual genera Paramonodictys and Paratrimmatostrom in having muriform, top-shaped, reddish-brown to dark brown conidia with a protruding basal cell; truncate to slightly rounded at the base. The conidia of Paramonodictys are globose or subglobose, olivaceous brown to dark brown, broadly rounded at apex whereas Paratrimmatostrom has helicoid, cylindrical, sigmoid, or reniform conidia.

Figures

FIGURE 1. RAxML tree based on combined ITS, LSU, SSU and TEF1-α sequence data.Bootstrap support values for Maximum likelihood (ML) and Bayesian posterior probabilities (BYPP) ≥0.95 are indicated above the nodes as ML/PP. The tree is rooted with Melanomma pulvis-pyrius (CBS 124080). The ex-type strains are shown in bold and new isolates are in red.