Trichinella spp

Kjaer, Lene Jung, Jensen, Laura Mark, Chri, Marian, el, Ren, Bødker, e & Petersen, Heidi Huus, 2021, The raccoon dog (Nyctereutes procyonoides) as a reservoir of zoonotic diseases in Denmark, International Journal for Parasitology: Parasites and Wildlife 16, pp. 175-182 : 177

2.2.3. Trichinella spp

Muscles from thighs were analysed for Trichinella spp . larvae by the magnetic stirrer method for pooled sample digestion according to Mayer-Scholl et al. (2017). Briefly, 20 g individual muscle samples from 10 animals were mixed with digestion fluid. The mixture was then stirred for a maximum of 60 min at 45 ◦ C, subsequently sieved and allowed to sediment for 30 min. The sediment was collected and allowed to sediment for another 10 min. The supernatant was removed and the sediment was analysed for presence of Trichinella spp . larvae by stereomicroscopy at × 20 magnification using a Leica M125 stereo microscope (Leica Microsystems A/S, Brønshøj, Denmark).