Aphidura pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013

Barjadze, Shalva, 2019, A new species of Aphidura Hille Ris Lambers, and additional data for Aphidura pakistanensis (Hemiptera: Aphididae) from Republic of Georgia, Zootaxa 4683 (3), pp. 421-430 : 425-427

Aphidura pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013

Figs 5–8 , Tables 1–2

Intraspecific variation in apterous viviparous females of A. pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013

Material examined. 6 apt. on 3 slides, coll. no. 13324A, GEORGIA, Mtskheta–Mtianeti Region, Mtskheta Municipality, Saguramo Range, Zedazeni Mountain, 41°52’14.45”N, 44°45’59.15”E, 1162 m alt., 12.viii.1969, on Dianthus , leg. J. Holman, det. Sh. Barjadze.

Aphidura pakistanensis was described based on four apterous viviparous females from a single locality in Pakistan ( Nieto Nafría et al. 2013). Later it was found in Iran ( Barjadze et al. 2017). One apterous viviparous specimen of the above-mentioned species from Iran is morphologically similar in length of SIPH (0.211 mm) and CAUDA (0.142 mm) to the apterae of the Pakistani population. However, (1) the PT/ANT VI b ratio in the Iranian specimen is higher (2.91) than in Pakistani (2.00–2.39) ones, (2) on the discal part of the subgenital plate of the Iranian specimen there are 3 setae, while only 2 setae are observed in Pakistani specimens and (3) ABD TERG VIII has one STu in the Iranian specimen, while STu are absent in Pakistani ones ( Nieto Nafría et al. 2013). Differences between apterous viviparous females from Georgian (fig. 5) and Pakistani populations in lengths of ANT III, HTII, MSL ABD TERG II–IV, SIPH and CAUDA, in ratios between MSL ABD TERG II–IV/ANT III BD, CAUDA L/SIPH L and CAUDA L/CAUDA W and in number of discal setae on subgenital plate should be considered as geographical and environmentally–induced differences (see Table 2 ).

Alate viviparous females of Aphidura pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013

Material examined. 11 al. on 6 slides, coll. no. 13324A, GEORGIA, Mtskheta–Mtianeti Region, Mtskheta Municipality, Saguramo Range, Zedazeni Mountain, 41°52’14.45”N, 44°45’59.15”E, 1162 m alt., 12.viii.1969, on Dianthus , leg. J. Holman, det. Sh. Barjadze. 5 al. on 3 slides are deposited at IECA; 4 al. on 2 slides are deposited at IZISU and 2 al. on 1 slide are deposited at the BMNH.

Description. Alate viviparous female (n=11). Color in life: unknown. Pigmentation on slide: ANT segments brown except basal part of ANT III, which is paler; head, thorax and brown; coxae dusky; 1/3 0r 1/2 of femora brown apically, while dusky basally; tibiae with brown apical and basal parts and dusky middle section; tarsi brown; SIPH, subgenital and anal plates and CAUDA brown; presiphuncular and postsiphuncular and sclerites are merged and make brown ring around SIPH; brown transverse bands present on ABD TERG VI–VIII; brown transverse band on ABD TERG V–VI complete or broken; large pale window present between dark transverse bands on ABD TERG V–VI; dark intersegmental sclerites on abdomen always present; abdomen with large, brown marginal sclerites on ABD TERG II–IV; stigmal plates brown (figs 6–8). Body spindle–shaped. Frons w–shaped. Head with rugosity lines and spinules.ANT 6–segmented (fig. 6). ANT tubercles well developed (figs 7 and 8). ANT cuticle imbricated. Rostrum short, reaching to middle coxae. Setae on body dorsum, antennae, and most of those on legs thick, with apices blunt or slightly capitate. Setae on the ventral side of abdomen long and pointed. ABD TERG II–VI with 0–1 MTu. ABD TERG VII with 0 Stu and ABD TERG VIII with 0–2 Stu. SIPH slightly to distinctly swollen on the distal halves, with spinulose imbrication, distinct preapical incision and flange (fig. 8). Cauda elongate triangular (fig. 8). Other characters as in apterous viviparous female. Measurements, ratios and chaetotaxy are provided in Table 1 .

Differential diagnosis. Alate viviparous females of A. pakistanensis have sec. rhin. on ANT IV like other four species of Aphidura : A. acanthophylli Remaudière, 1989 , A. mordvilkoi Shaposhnikov, 1984 , A. pujoli (Gómez- Menor Ortega, 1950) and A. togaica Kadyrbekov, 2013 . Alate females of A. acanthophylli differ from those by the presence of conical tubercles on the abdominal dorsum ( Remaudière 1989). Alate females of A. mordvilkoi have a central patch on the abdomen in front of SIPH, while there is no central patch on the abdomen in alatae of A. pakistanensis ( Shaposhnikov 1984) . Length of SIPH in alate viviparous females of Dianthus –feeding A. pujoli is more than 0.20 mm and ANT III has 22–25 sec. rhin., while in alatae of A. pakistanensis SIPH is less than 0.20 mm and ANT III has 32–48 sec. rhin. ( Gómez-Menor Ortega 1950; Blackman & Eastop 2019). Alate females of A. togaica have 15–20 setae on the posterior margin of subgenital plate, 0–1 sec. rhin. on ANT IV and a pale cauda, while alatae of A. pakistanensis have 10–16 setae on the posterior margin of subgenital plate, 2–10 sec. rhin. on ANT IV and a dark cauda ( Kadyrbekov 2013).

Figures

FIGURE 5–8. Aphidura pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013 from Georgia. 5. Habitus of apterous viviparous female (bar: 500 μm); Alate viviparous female. 6. ANT IV–VI (bar: 100 μm); 7. Head with ANT I–III (bar: 200 μm); 8. Habitus (bar: 500 μm).

Tables

TABLE 2. Comparison of apterous viviparous females from Pakistani and Georgian populations of Aphidura pakistanensis Nieto Nafría, Mier Durante & Remaudière, 2013. Note: Information about apterous viviparous females of A. pakistanensis from Pakistani population is provided from Nieto Nafría et al. (2013).

species	A. pakistanensis from Pakistan	A. pakistanensis from Georgia
	(apt., n=4)	(apt., n=6)
Characters
ANT III	0.32–0.49	0.28–0.33
HTII L	0.11–0.13	0.13–0.15
MSL ABD TERG II–IV	0.010	0.008–0.009
SIPH L	0.17–0.20	0.13–0.16
CAUDA L	0.11–0.14	0.15–0.16
Ratios
MSL ABD TERG II–IV/ANT III BD	0.4–0.6	0.3–0.4
CAUDA L/SIPH L	0.55–0.82	0.95–1.22
CAUDA L/CAUDA W	0.71–1.00	0.99–1.08
Number of setae
Subgenital plate–discal	2	3–5