Haemoproteus (Parahaemoproteus) nucleocondensus, Križanauskienė, Asta, Iezhova, Tatjana A., Palinauskas, Vaidas, Chernetsov, Nikita & Valkiūnas, Gediminas, 2012
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publication ID |
https://doi.org/ 10.5281/zenodo.282145 |
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DOI |
https://doi.org/10.5281/zenodo.5630398 |
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persistent identifier |
https://treatment.plazi.org/id/03CA2B08-FFCE-FFF1-FF2F-F9BEA2FA9CD5 |
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treatment provided by |
Plazi |
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scientific name |
Haemoproteus (Parahaemoproteus) nucleocondensus |
| status |
sp. nov. |
Haemoproteus (Parahaemoproteus) nucleocondensus n. sp.
( Figure 1 View FIGURE 1 , Table 1 View TABLE 1 ).
Young gametocytes ( Figure 1 View FIGURE 1 , a, b): Develop in mature erythrocytes. Earliest forms not observed, but growing gametocytes are numerous in type material. With development (size greater than erythrocyte nuclei), gametocytes closely associate with nuclei of infected erythrocytes; they do not touch envelope of erythrocytes along entire margin ( Figure 1 View FIGURE 1 , b), a characteristic feature of this species’ development. Parasite nucleus small, central or subcentral ( Figure 1 View FIGURE 1 , a, b) in position. Pigment granules medium size (0.5–1.0 μm), roundish or slightly oval, darkbrown or black, and frequently grouped ( Figure 1 View FIGURE 1 , a). Outline even ( Figure 1 View FIGURE 1 , b) or slightly wavy ( Figure 1 View FIGURE 1 , a). Volutin granules not seen. Growing gametocytes do not displace erythrocyte nuclei laterally ( Figure 1 View FIGURE 1 , a, b).
Macrogametocytes ( Figure 1 View FIGURE 1 , c–h): Gametocytes grow along nuclei of erythrocytes, not displacing or only slightly displacing nuclei laterally. The majority of growing ( Figure 1 View FIGURE 1 , c, d) and fully-grown gametocytes ( Figure 1 View FIGURE 1 , g, h) are closely associated with both nucleus and envelope of erythrocytes, but advanced gametocytes, which do not touch envelope of erythrocytes along entire margin present ( Figure 1 View FIGURE 1 , d), a distinctive feature of this species’ development. Growing gametocytes slightly enclose erythrocyte nuclei with their ends; do not fill erythrocytes up to their poles ( Figure 1 View FIGURE 1 , c–f). Fully-grown gametocytes occupy nearly all cytoplasmic space on poles of erythrocytes (see Figure 1 View FIGURE 1 , g, h and also microgametocyte in Figure 1 View FIGURE 1 , l). Fully-grown gametocytes halteridial, and only slightly enclose erythrocyte nuclei with their ends and do not displace or only slightly displace them laterally ( Figure 1 View FIGURE 1 , g, h). Parasite nucleus compact, small ( Table 1 View TABLE 1 ), markedly variable in shape, and central or sub-central ( Figure 1 View FIGURE 1 , c–h) in position. Nucleolus not observed. Pigment granules roundish or slightly oval, dark-brown, of medium size (0.5–1 µm), randomly scattered throughout cytoplasm ( Figure 1 View FIGURE 1 , d, e, h) or grouped ( Figure 1 View FIGURE 1 , c, f, g). Individual granules variable in size (non-sister-size). Outline of gametocytes usually even ( Figure 1 View FIGURE 1 , c, d, e, f, h), or occasionally slightly wavy ( Figure 1 View FIGURE 1 , g). Cytoplasm blue, homogeneous in appearance, usually lacks visible vacuoles, and stains more intensively in the ends of gametocytes. Volutin granules not seen.
Microgametocytes ( Figure 1 View FIGURE 1 , i–l): General configuration as for macrogametocytes, with main haemosporidian sexually dimorphic characters. Parasite nucleus markedly condensed and small ( Table 1 View TABLE 1 ); these 2 attributes of microgametocyte nuclei are distinctive morphological characters of this species. In the majority of fully-grown gametocytes, nuclei are of irregular shape, central or sub-central in position, do not closely associated with parasite pellicle ( Figure 1 View FIGURE 1 , i–l). Area of microgametocyte nuclei is significantly less than in macrogametocytes ( Table 1 View TABLE 1 , P <0.001), a rare character for bird haemosporidian parasites.
Feature Measurements a
Feature Measurements a a All measurements are given in micrometers. Minimum and maximum values are provided, followed in parentheses by the arithmetic mean and standard deviation.
b Measurements are given according to Valkiūnas et al. (1994), except for area of macrogametocytes and their nuclei, which are original data.
c NDR = nucleus displacement ratio according to Bennett & Campbell (1972).
TABLE 1. Morphometric parameters of gametocytes and host cells of Haemoproteus payevskyi (lineage hRW 1) and Haemoproteus nucleocondensus sp. nov. (lineage hGRW 1).
| Uninfected erythrocyte Length Width Area Uninfected erythrocyte nucleus | H. payevskyi (n=31)b 10.3–12.7 (11.7±0.5) 6.1–7.4 (6.7±0.3) - | H. nucleocondensus (n=21) 11.3–14.6 (13.2±0.9) 5.6–7.3 (6.6±0.4) 52.4–78.3 (70.9±5.5) |
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| Length Width Area | 5.2–6.0 (5.5±0.2) 1.8–2.6 (2.2±0.2) - | 5.5–7.3 (6.0±0.4) 1.9–2.4 (2.2±0.2) 9.6–12.4 (11.2±0.8) |
| Macrogametocyte Infected erythrocyte Length | 10.8–12.9 (11.9±0.6) | 12.6–16.2 (14.5±0.8) |
| Width Area Infected erythrocyte nucleus | 5.8–7.4 (6.7±0.2) - | 5.8–7.0 (6.5±0.4) 56.6–91.8 (76.1±7.4) |
| Length Width Area Gametocyte | 4.6–6.4 (5.2±0.2) 1.9–2.8 (2.2±0.1) - | 5.8–7.3 (6.6±0.4) 2.0–2.7 (2.3±0.2) 10.7–14.4 (12.4±0.9) |
| Length Width Area Gametocyte nucleus | 9.8–12.0 (10.5±0.4) 2.4–3.4 (2.9±0.4) 27.4–36.5(32.0±2.7) | 11.9–15.1 (13.3±0.8) 1.8–2.8 (2.3±0.2) 27.7–40.6 (35.5±3.3) continued next page |
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