Cunninghamella echinulate, ATCC

Eliwa, Duaa, Albadry, Mohamed A., Ibrahim, Abdel-Rahim S., Kabbash, Amal, Meepagala, Kumudini, Khan, Ikhlas A., El-Aasr, Mona & Ross, Samir A., 2021, Biotransformation of papaverine and in silico docking studies of the metabolites on human phosphodiesterase 10 a, Phytochemistry (112598) 183, pp. 1-12 : 6

3.4. Microbial transformation using Cunninghamella echinulate ATCC 18968

Biotransformation of papaverine using Cunninghamella echinulate ATCC 18968 produced one metabolite. The residue (800 mg) was dissolved in 30 ml of methanol/dichloromethane mixture (1:1) and adsorbed onto 800 mg Celite and dried. The adsorbed sample was placed onto a glass column (110 × 2 cm) packed with silica after making a slurry in dichloromethane. The column was gradiently eluted with dichloromethane 100% then dichloromethane: methanol (98.5:1.5, 98:2) and 10 ml fractions were collected. Fractions 40–60 were pooled together to give compound 1. Further purification was achieved using sephadex LH-20 column, as determined by TLC. The metabolite was obtained in the form of white powder (70 mg, R f 0.6 S 2).