Simulium (Chirostilbia) jefersoni Hamada, Hernández, Luz & Pepinelli, 2006

Hamada, Neusa, Hernandez, Luis M., Luz, Sergio Luiz Bessa & Pepinelli, Mateus, 2006, Simulium (Chirostilbia) jefersoni, a new species of black fly (Diptera: Simuliidae) from the state of Bahia, Brazil, Zootaxa 1123, pp. 21-37 : 23-32

publication ID

https://doi.org/ 10.5281/zenodo.171769

DOI

https://doi.org/10.5281/zenodo.6255795

persistent identifier

https://treatment.plazi.org/id/03DC87FB-0212-207F-FEA2-FEC7BABEF8A0

treatment provided by

Plazi

scientific name

Simulium (Chirostilbia) jefersoni Hamada, Hernández, Luz & Pepinelli
status

sp. nov.

Simulium (Chirostilbia) jefersoni Hamada, Hernández, Luz & Pepinelli View in CoL New Species

( Figs. 1–45 View FIGURES 1 – 4 View FIGURES 5 – 13 View FIGURES 14 – 17 View FIGURES 18 – 24 View FIGURES 25 – 28 View FIGURES 29 – 36 View FIGURE 37 View FIGURE 38 View FIGURES 39 – 45 )

Female ( Figs. 1–17 View FIGURES 1 – 4 View FIGURES 5 – 13 View FIGURES 14 – 17 ). General body color dark brown to black (specimens recovered from alcohol) ( Figs. 1–4 View FIGURES 1 – 4 ). Body length (specimens in alcohol) 2.6–3.0 mm (n = 3); thorax lateral length 0.9 mm (n = 3). Wing length 1.3–1.8 mm (n = 3); wing width 0.7–0.8 mm (n = 3).

Head dichoptic with dark red eyes and fronto­ocular triangle well developed ( Fig. 8 View FIGURES 5 – 13 ). Frons, clypeus, and occiput black, with silver pruinosity; clypeus and frons covered with dark, erect setae. Antennae with silver pubescence, 0.40–0.44 mm in length; scape and pedicel pale brown, remaining segments dark brown ( Fig. 6 View FIGURES 5 – 13 ). Maxillary palpus dark brown; sensory vesicle elongated occupying more than 1/3 length of palpomere III; palpomere V twice length of palpomere III and IV ( Fig. 5 View FIGURES 5 – 13 ). Mandible with 12 external serrations and 34–38 internal teeth. Lacinia with 25 or 26 retrorse teeth. Cibarium with well­developed, sclerotized cornuae, without teeth ( Fig. 7 View FIGURES 5 – 13 ). Thorax with scutum black covered by evenly arranged, recumbent, whitish setae; posterior margin with short, recumbent, whitish setae. Scutal pattern varying with illumination. With anterior illumination, thorax black with 1+1 median and 1+1 sublateral, silver pruinose vittae extending from anterior to posterior margin of thorax and black lyre­shaped pattern; humeri pale brown with faint gray pruinosity; lateral and posterior margins black ( Fig. 1 View FIGURES 1 – 4 ). With posterior illumination, lyre­shaped pattern silver pruinose on black scutum; humeri weakly pale brown and lateral margins weakly silver pruinose; posterior margin black ( Fig. 2 View FIGURES 1 – 4 ). Anepisternum dark brown; katepisternum light brown. Scutellum dark brown with recumbent whitish setae interspersed with long, black bristles. Postnotum dark brown with silver pruinosity. Pleura dark brown with silver pruinosity. Costa of wing with sparse distribution of spines and setae. Subcosta with line of few setae up to half length of vein ( Fig. 9 View FIGURES 5 – 13 ). Radius with line of setae intermixed with spines apically; basal section bare. Basal tuft of long, dark setae ( Fig. 9 View FIGURES 5 – 13 ). Leg coloration and proportions as in Figs. 10–12 View FIGURES 5 – 13 . Fore leg with coxa, trochanter, femur, and tibiae yellow; apex of tibia weakly pale brown; basitarsus and tarsomeres I–IV dark brown ( Fig. 10 View FIGURES 5 – 13 ). Middle leg pale yellow except apical two­thirds of basitarsus and tarsomeres I–IV dark brown to black and one­third of basitarsus whitish ( Fig. 11 View FIGURES 5 – 13 ). Hind leg with coxa, apex of femur, half of tibiae, apical onethird of basitarsus, and tarsomeres I–IV dark brown, remainder of hind leg pale yellow ( Fig. 12 View FIGURES 5 – 13 ). Claws curved with basal tooth ( Fig. 13 View FIGURES 5 – 13 ). Halteres cream with brown base. Abdomen with tergites I–IX dark brown to black ( Figs. 3, 4 View FIGURES 1 – 4 ); tergite II silver pruinose on anterolateral margins. Basal fringe with thin, long, golden hairs. Tergal plates developed; sternal plates undeveloped. Sternites grayish black; genitalia dark brown. Eighth sternite weakly sclerotized with irregularly distributed setae on posterior margin; hypogynial valves (= gonapophyses) nearly same length as eighth sternite at its midpoint, subtriangular, membranous except weakly sclerotized on internal margins ( Fig. 14 View FIGURES 14 – 17 ). Cercus subquadrangular, covered with long, brown setae; anal lobe (= paraproct) subtriangular, nearly one and one­half times longer than cercus, sclerotized and covered by long setae basally and small hairs on distal region of posterior margin ( Fig. 15 View FIGURES 14 – 17 ). Genital fork stout, sclerotized with apical termination of stem expanded; termination of lateral arm almost straight; anterior process developed, rounded apically; posterior processes poorly developed ( Fig. 16 View FIGURES 14 – 17 ). Spermatheca globular, with internal spicules in groups of 2–4 ( Fig. 17 View FIGURES 14 – 17 ); spermathecal duct and area of attachment unpigmented.

Male ( Figs. 18–28 View FIGURES 18 – 24 View FIGURES 25 – 28 ). General body color dark brown (specimen recovered from alcohol) ( Figs. 18, 19, 23, 24 View FIGURES 18 – 24 ). Body length (specimens in alcohol) 2.2–3.0 mm (n = 3); thorax lateral length 0.72–0.78 mm (n = 3). Wing length 1.6–2.5 mm (n = 3); wing width 0.9 mm (n = 3).

Head holoptic with dark red eyes. Antenna with whitish pubescense, 0.50 mm in length; scape, pedicel, and first flagellomere light brown, remaining flagellomeres dark brown ( Fig. 20 View FIGURES 18 – 24 ). Palpus dark brown, sensory vesicle small, occupying less than 1/5 of palpomere III; palpomere V about 1.6 times as long as palpomeres III and IV ( Fig. 21 View FIGURES 18 – 24 ). Thorax with scutum black covered with recumbent golden hairs. Scutal pattern varies slightly with light incidence: with anterior light source thorax black with 1+1 submedian, silver pruinose cunae on anterior one­third ( Fig. 18 View FIGURES 18 – 24 ). With light source posterior to specimen, thorax black ( Fig. 19 View FIGURES 18 – 24 ). Humeri pale brown; lateral and posterior margins of scutum pruinose [best seen when specimen viewed laterally]. Scutellum dark brown with recumbent whitish hairs interspersed with long, erect black hairs on posterior margin. Postnotum dark brown to black with silvery gray pruinosity. Anepisternum and katepisternum dark brown. Wing setation and leg coloration as in female, except claws without basal tooth ( Fig. 22 View FIGURES 18 – 24 ). Abdomen ( Figs. 23, 24 View FIGURES 18 – 24 ) with tergite I black with posterior margin silver pruinose; basal fringe with long, thin, golden hairs; tergite II black in median region and pale brown laterally; tergites III–IV black; tergites V–IX black mesally and grayish in lateral margins; tergites II (anteriorly), IV–VII with silver pruinosity on ventrolateral margin; in some specimens, silver pruinosity also on tergites VIII and IX [best seen on specimens in lateral view]. Sternal plates undeveloped. Genitalia black; gonocoxite subquadrangular ( Fig. 28 View FIGURES 25 – 28 ); gonostyle conical (finger­like) ( Fig. 28 View FIGURES 25 – 28 ), nearly as long as gonocoxite at midpoint, with ridge in median region and 2 spiniform setae apically [visible only at high magnification]; gonocoxite and gonostyle covered with long setae. Ventral plate sclerotized, with ventral margin almost straight, without keel and covered by small hairs; basal arms short and deeply sclerotized ( Fig. 27 View FIGURES 25 – 28 ). Median sclerite Y shaped with distinct incision in apical one­third ( Fig. 26 View FIGURES 25 – 28 ). Paramere with developed and sclerotized basal process and numerous stout teeth centrally ( Fig. 25 View FIGURES 25 – 28 ).

Pupa ( Figs. 29–38 View FIGURES 29 – 36 View FIGURE 37 View FIGURE 38 ). Cocoon length dorsally 2.8–3.8 mm (mean = 3.2 mm, SD = 0.28, n = 10), ventrally 3.8–5.4 mm (mean = 4.5 mm, SD = 0.39, n = 10); pupa length 2.6–3.9 mm (mean = 3.1 mm; SD = 0.43, n = 10); gill length 1.5–2.5 mm (mean = 1.9 mm, SD = 0.38, n = 8).

Cocoon shoe shaped, dark brown composed of thick coalesced fibres posteriorly and distinct, loop­like fenestrations anteriorly ( Figs. 29, 30 View FIGURES 29 – 36 ). Frontoclypeus with 3+3 long, multiramous frontal and 1+1 long, multiramous, dorsal trichomes ( Fig. 34 View FIGURES 29 – 36 ); frontoclypeus with distinct groups of platelets mesally, 1+1 dorsolaterally and 2 or 3 platelets in 2 groups laterally in frontal region, respectively ( Fig. 33 View FIGURES 29 – 36 ); tubercles rounded, densely distributed ( Fig. 36 View FIGURES 29 – 36 ) over entire frontal and dorsal region. Thorax with 5 pairs of long, multiramous ( Fig. 32 View FIGURES 29 – 36 ) trichomes near margin of dorsal cleft, 2+2 multiramous and 1 simple trichomes at gill base, one small, bifid or simple trichome on ventral margin, and 1 bifid or trifid trichome in median region of thorax; tubercles rounded, densely distributed over entire surface. Antennal sheath with transversal punctuations ( Fig. 35 View FIGURES 29 – 36 ). Gill filaments pale yellowish with 8 forwardly directed filaments, arranged in 3 dimensions, curving at midpoint and directed inward apically. Gill configuration with main trunk short, giving rise to 3 sets of primary branches, dorsal and ventral (internal) primary branches each consisting of 3 secondary branches; external set consists of 2 secondary branches; all filaments bifurcate at different heights near base of gill. Filaments stout basally and becoming narrower toward apex, rounded distally, without spicules on surface; edges weakly crenate. All gill filaments approximately same length ( Fig. 31 View FIGURES 29 – 36 ). Abdominal tergite I ( Figs. 37 View FIGURE 37 a–e) with 1+1 submedian, simple trichomes. Tergite II with 4+4 submedian, spiniform setae in longitudinal row, 2+2 small, simple trichomes anterior to most external spiniform setae and 1+1 simple trichome on lateral margin ( Fig. 37 View FIGURE 37 a). Tergites III and IV with 4+4 submedian, simple hooks in longitudinal row ( Figs. 37 View FIGURE 37 b, 37c), 1+1 small, simple trichomes anterior to most lateral hooks, and 1+1 small, simple trichomes on lateral margin. Tergite V with 1+1 sublateral, small, simple trichomes, and 1+1 small, simple trichomes on anterior margin; tergite VI with 1+1 submedian and 1+1 sublateral, small trichomes. Tergite VII with 1+1 sub­median and 1+1 sublateral, small, simple trichomes. Tergite VIII with 1+1 submedian, simple or bifid, and 1+1 sublateral, simple trichomes, and spine combs distinctly resembling teeth on anterior margin ( Fig. 37 View FIGURE 37 e). Tergite IX weakly sclerotized, with 1+1 small spines. Spine combs on anterior margin of tergites II, VI–IX ( Fig. 37 View FIGURE 37 d). Abdominal sternite III ( Figs. 38 View FIGURE 38 a–d) with 1+1 submedian and 2+2 sublateral, small, simple trichomes; sternite IV with 1+1 submedian and 2+2 sublateral, small, simple trichomes; sternite V with 2 +2 close, bifid or trifid hooks in row and 2+2 simple trichomes anterior to most lateral hooks ( Fig. 38 View FIGURE 38 b); sternites VI and VII with 2+2 well separated, simple, bifid or trifid hooks ( Figs. 38 View FIGURE 38 c, 38d), sternite VII with 1+1 simple trichomes on lateral margin; sternite VIII without hooks; sternite IX weakly sclerotized. Abdominal sternites III–IX with spine combs on anteromedian margin ( Fig. 38 View FIGURE 38 a).

Larva (last instar) ( Figs. 39–45 View FIGURES 39 – 45 ). Body length: 5.8–6.6 mm (mean = 6.2 mm, SD = 0.2, n = 10); head capsule lateral length: 0.6–0.8 mm (mean = 0.7 mm, SD = 0.06, n = 10); dorsal width of head capsule: 0.5–0.7 mm (mean = 0.6 mm, SD = 0.05, n = 10).

General body coloration pale gray (in Carnoy’s solution); form as in Fig. 39 View FIGURES 39 – 45 . Head mainly pale brown, numerous small setae present on all surfaces and head capsule slightly wrinkled. Head pattern positive ( Fig. 43 View FIGURES 39 – 45 ). Cervical sclerites small, elliptical, free in membrane. Postgenal cleft subtriangular, wider basally ( Figs. 41, 44 View FIGURES 39 – 45 ). Postgenal bridge 0.7 times as long as hypostoma ( Fig. 41 View FIGURES 39 – 45 ). Hypostoma with strongly pigmented anterior margin and 9 apical teeth ( Fig. 40 View FIGURES 39 – 45 ): median tooth simple and more prominent than sublateral teeth, 3+3 rows of sublateral teeth, middle tooth smaller than remaining teeth, 1+1 lateral teeth nearly same height as median tooth, 2 poorly developed paralateral teeth, and 3 small lateral serrations; hypostoma with 1+1 line of 6 or 7 lateral setae parallel to lateral margin and 1+1 long and 1+1 or 2+2 short setae in posterior region of hypostoma near hypostomal groove. Subesophageal ganglion not pigmented. Antennae ( Fig. 42 View FIGURES 39 – 45 ) as long as labral fan stalk; antennal segments brown, except whitish apical one­third of median and basal region of distal segment; segments proportions (proximal, medial and distal) approximately 0.7–0.9: 0.4–0.6: 1.2–1.3 (n = 3). Mandible with 3 apical teeth, basal tooth more prominent than remaining teeth, second comblike teeth with first tooth longer than second and third, mandible with 9 internal teeth, and mandibular serration with anterior teeth longer than posterior; mandibular brushes well developed; mandible with small, simple setae near external margin and 2 prominent, simple trichomes at base of apical mandibular brush. Lateral mandibular process simple and thick. Maxillary palp heavily pigmented, nearly 3 times as long as wide at base. Cephalic fan with 43–47 rays. Thorax pale dorsally and grey ventrally. Proleg with plate heavily sclerotized with band of more than 40 processes of nearly 13 hooks (n = 1). Pupal gill histoblast dark brown with 8 filaments. Abdomen usually completely gray dorsally, progressively paler ventrally, last segments white; faint segmental banding visible dorsally ( Fig. 39 View FIGURES 39 – 45 ). Cuticle mainly lacking setae. Ventral nerve cord pale gray. Ventral posterior tubercles absent. Anterodorsal arms of anal sclerite shorter in length than posteroventral arms ( Fig. 45 View FIGURES 39 – 45 ). Posterior proleg bearing approximately 100 rows of up to 13 or 14 hooks (n = 2). Anal papillae with 3 branches, each with approximately 7 or 8 finger­shaped lobes of same length (n = 1).

Type Material

Holotype ( INPA): Brazil, Bahia State, Palmeiras County. Parque Nacional Chapada Diamantina: Cachoeira da Fumaça stream (#32), 12o36’S 41o27’W, 31.vii.2005 (N. Hamada, M. Pepinelli and V.L. Landeiro) ­ female (pinned), with pupal exuviae in glycerine.

Paratypes: Brazil, Bahia State, Palmeiras County. Parque Nacional Chapada Diamantina: Cachoeira da Fumaça stream (#32), 12o36’S 41o27’W, 31.vii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 2 pupae (P), 2 larvae (L) in ethanol, 1 male (M) pinned, with pupal exuviae in glycerine ( INPA); small tributary of Mucujezinho stream (#26), 12o28’S 41o27’W, 07.vii.2003 (N. Hamada & J. Silva) ­ 2P, 2L in ethanol ( INPA); Galinha stream (#29), Bomba village (Vale do Capão), Gerais do Vieira, 12o40’S 41o29’W, 08.vii.2003 (N. Hamada & J. Silva) ­ 3L in ethanol ( INPA); Batista River (#30), Pousada do Capão, 12o37’S 41o29’W, 08.vii.2003 (N. Hamada & J. Silva) ­ 2L in ethanol ( INPA); Sobradinho stream (#34), Lençóis­Capão trail, Gerais do Morrão, 12o32’S 41o28’W, 10.vii.2003 (N. Hamada & J. Silva) ­ 1L, 1P in ethanol, 1F pinned with pupal exuviae in glycerine ( INPA); Águas Claras stream, Gerais do Morrão, Lençóis­Capão trail, 12o31’S 41o28’W, 10.vii.2003 (#35) (N. Hamada & J. Silva) ­ 1L, 2P in ethanol ( INPA), 26.vii.2005 (#14) (N. Hamada & M. Pepinelli) ­ 2P ethanol ( INPA); Morrão stream (#13), Morrão trail, 12o31’S 41o28’W, 26.vii.2005 (N. Hamada & M. Pepinelli) ­ 1L, 2P in ethanol ( INPA); headwater of Conceição dos Gatos stream (#15), Morrão trail, 12o32’S 41o28’W, 26.vii.2005 (N. Hamada & M. Pepinelli) ­ 1P in ethanol ( INPA); Mucujezinho stream (#17), 12o28’S 41o27’W, 27.vii.2005 (N. Hamada & M. Pepinelli) ­ 2L, 3P in ethanol ( INPA). Lençóis County, Parque Nacional Chapada Diamantina: Cachoeirinha stream (#32), 12o33’S 41o24’W, 09.vii.2003 (N. Hamada & J. Silva) ­ 2P in ethanol ( INPA), 25.vii.2005 (N. Hamada & M. Pepinelli) ­ 1P in ethanol ( INPA). Andaraí County, Parque Nacional Chapada Diamantina: Piabas River (below erosion area) (#39), 12o51’S 41o18’W, 11.vii.2003 (N. Hamada & J. Silva) ­ 1L, 2P in ethanol ( INPA); Piabas River (#43), 12o57’S 41o16’W, 04.viii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 2L, 2P in ethanol ( INPA); Coisa Boa River (#40), 12o51’S 41o18’W, 11.vii.2003 (N. Hamada & J. Silva) ­ 1P in ethanol ( INPA); Roncador River (#41), “off road” track between Andaraí and Lençóis, 12o41’S 41o21’W, 11.vii.2003 (N. Hamada & J. Silva) ­ 2L, 3P in ethanol, 1F pinned ( INPA); Serrano River (#9), 12o33’S 41o23’W, 25.vii.2005 (N. Hamada, M. Pepinelli) ­ 2L, 1P in ethanol ( INPA); Pombos waterfall (#38), Igatu, 12o54’S 41o19’W, 03.viii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 2P in ethanol ( INPA). Mucugê County, Parque Nacional Chapada Diamantina: Cumbuca River (#42), 12o59’S 41o21’W, 12.vii.2003 (N. Hamada & J. Silva) ­ 3P in ethanol, 1M pinned ( INPA); 1F, 1M pinned (genitalia on slide), 2F, 2M, 7P, 4L in slides, several P in ethanol, several L in Carnoys ( BMNH); stream in the access road to Sibéria waterfall (#45), 12 o56’S 41o21’W, 15.vii.2003 (N. Hamada & J. Silva) ­ 3P in ethanol ( INPA); Pimenteiras stream (#46), in the access road to Sibéria waterfall, 12o57’S 41o21’W, 15.vii.2003 (N. Hamada & J. Silva) ­ 1L, 4P in ethanol ( INPA); first stream on the access road to Sibéria waterfall (#41), 12o58’S 41o21’W, 04.viii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 2L, 2P in ethanol ( INPA). Itaitê County, Urubu River (#24), Chapadinha, Rumo village, 13o11’S 41o10’W, 29.vii.2005 (N. Hamada, M. Pepinelli) ­ 2P in ethanol, 1F pinned ( INPA); Barrigudinha River (#26), Rumo village, 13o13’S 41o08’W, 29.vii.2005 (N. Hamada, M. Pepinelli) ­ 2L, 2P in ethanol, 2F, 2M pinned ( INPA). Ibicoara County, Parque Muncipal Natural do Espalhado, Espalhado River (# 52), 13o19’S 41o09’W, 08.viii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 4P in ethanol, 1F, 1M pinned ( INPA). Rio de Contas County, Mocotó River (#57), Mato Grosso village, 13o25’S 41o50’W, 10.viii.2005 (N. Hamada, M. Pepinelli & V.L. Landeiro) ­ 2L, 3P in ethanol ( INPA).

Etymology: This species is named in honor of Jeferson Oliveira da Silva (INPA/ CPEN), an ethical and dedicated professional and a good friend, who has been working tirelessly with N. Hamada in the field and laboratory.

Taxonomic discussion and diagnosis: Simulium jefersoni n. sp. is placed in the subgenus Chirostilbia based on the combination of characters given by Coscarón (1987, 1991). Within Chirostilbia , this species might be included in the S. pertinax species group by having females with a black thorax and tarsal claws with a basal tooth ( Coscarón 1987). However, the males have a ridge on the median region of the gonostyle, as in the species of the S. subpallidum group. The adults in the subgenus Chirostilbia are difficult to separate, especially those species in the S. pertinax group, without examination of the pupal gill configuration. Comparisons, especially with species in image archives in the BMNH, identified black fly specimens at INPA and BMNH, and published illustrations of known species of Chirostilbia , indicate that S. jefersoni n. sp. is morphologically similar to several species. The females are externally similar to females of S. acarayense Coscarón & Wygodzinsky , S. laneportoi Varg a s, S. papaveroi Coscarón , S. serranum Coscarón , S. spinibranchium Lutz , the black form of S. subpallidum Lutz , and S. striginotum Enderlein by having the scutum black with 1+1 median and 1+1 sublateral silver pruinose vittae and a black lyre­shaped pattern. However, the new species can be distinguished by the structure of the anal lobe ( Fig. 15 View FIGURES 14 – 17 ) and its length, which is nearly 1.5 times longer than the cercus. In the other species, the anal lobe is approximately 2.5 to 3 times longer than the cercus. The male thoracic pattern of S. jefersoni n. sp. is also similar to that of species in the S. pertinax species group, from which this species can be distinguished by the structure of the gonostyle and the ventral plate. In S. jefersoni n. sp., the gonostyle is conical and finger­like with a ridge ( Fig. 28 View FIGURES 25 – 28 ), and the ventral plate is distinctly rectangular, with the ventral margin nearly straight ( Fig. 27 View FIGURES 25 – 28 ), similar to that of S. acarayense ( Coscarón 1991) . The most reliable character for identifying S. jefersoni n. sp. is the structure of the cocoon and the number and configuration of the pupal gill filaments. The pupa of the new species has eight gill filaments ( Fig. 31 View FIGURES 29 – 36 ), which group this species with S. acarayense , S. empascae , S. laneportoi , S. papaveroi , S. pertinax , S. serranum , S. spinibranchium and S. subpallidum . However, S. jefersoni n. sp. can be separated by the shoe­shaped cocoon with prominent anterior fenestrations ( Figs. 29, 30 View FIGURES 29 – 36 ). In this respect, S. jefersoni n. sp. is similar to S. distinctum , S. friedlanderi , S. obesum Vulcano and S. riograndense , but the number of its gill filaments distinguishes it from these species, which have 10, 11,>100, and 10 filaments, respectively. The cocoon in S. empascae is also shoe­shaped, but the pupal gill filaments and the cocoon have different configurations. The pupa of S. jefersoni n. sp. also can be recognized by the set of three primary branches giving rise to three sets of secondary branches that curve at their midpoints and diverge inward apically ( Fig. 31 View FIGURES 29 – 36 ), and the thorax and frontoclypeus with prominent, multiramous trichomes ( Figs. 32, 34 View FIGURES 29 – 36 ). The pupae of the known eight­filamented species of Chirostilbia in the Neotropical Region have a different gill and trichome configuration pattern. The postgenal cleft of the lastinstar larva of S. jefersoni n. sp. is similar to that of S. serranum ( Coscarón 1981) , but both species can be distinguished by the different structure of the filaments of the gill histoblast.

Bionomics: Simulium jefersoni n. sp. has been collected only in the state of Bahia, Brazil, in Chapada Diamantina National Park and the surrounding area. The immature stages were collected from trailing vegetation, deciduous leaves, and rocks in areas with faster flow in 30 rock­bottomed streams, with width varying from 1 to 50 m. The streams had black water, mean water temperature of 21o C (SD = 2.0), median electrical conductivity below 20µS/cm, and mean pH of 4.5 (SD = 0.45). These streams were located at altitudes of 468 to 1345 m. Females were not collected biting humans.

INPA

Instituto Nacional de Pesquisas da Amazonia

Kingdom

Animalia

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Simuliidae

Genus

Simulium

GBIF Dataset (for parent article) Darwin Core Archive (for parent article) View in SIBiLS Plain XML RDF