Phaeotremella lactea Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 98

Phaeotremella lactea Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828782. Fig. 10I .

Etymology: the specific epithet lactea refers to the colony colour of this species.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.7– 4.0 × 4.4– 6.6 μm and single, budding is polar ( Fig. 10I ), a sediment is present. After 1 mo at 17 °C, a pellicle and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, ribitol, D-mannitol, D-glucitol, salicin, Dgluconate, succinate and myo-inositol are assimilated as sole carbon sources. L-sorbose, soluble starch, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, DL-lactate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), sodium nitrite (weak), L-lysine (weak) and ethylamine hydrochloride (weak) are assimilated as sole nitrogen sources. Cadaverine dihydrochloride is not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive (weak). Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Pha. lactea differs from the closely related species Pha. ovata in its inability to assimilate soluble starch, N- Acetyl-D-glucosamine, galactitol, Methyl-α- D-glucoside and cadaverine and its ability to assimilate raffinose, succinate and myo-inositol ( Table S1.12 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5810 T preserved in a metabolically inactive state, ex-type CBS 15574 = GPS20.4A1B).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.