Derxomyces longicylindricus Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 98

Derxomyces longicylindricus Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828781. Fig. 10G, H .

Etymology: the specific epithet longicylindricus refers to the long cylindrical vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are long cylindrical, 2.9–5.0 × 7.1– 22 μm and single, budding is polar ( Fig. 10G ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, wrinkled and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal to napiform, 2.4– -4.2 × 3.6–6.0 μm ( Fig. 10H ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose (weak), sucrose, maltose, cellobiose (weak), trehalose, melibiose, raffinose, melezitose, inulin, soluble starch (weak), D-xylose, L-arabinose, L-rhamnose, D-glucitol (delayed and weak), Methyl-α- D-glucoside and succinate (delayed and weak) are assimilated as sole carbon sources. Lactose, D-ribose, D-arabinose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate and sodium nitrite are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, De. longicylindricus differs from the closely related species De. linzhiensis in its inability to assimilate Darabinose, galactitol, D-mannitol and cadaverine and its ability to assimilate L-rhamnose, L-lysine and ethylamine ( Table S1.11 ).

Typus: China, Beibeng county, Motuo , Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5660 T preserved in a metabolically inactive state, ex-type CBS 13979 = XZ132E37A).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.