Kondoa arboricola Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 104-105

Kondoa arboricola Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828795. Fig. 12I, J .

Etymology: the specific epithet arboricola refers to the substrate origin of the type strain, tree.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.9– 5.0 × 7.1– 10.0 μm and single, budding is polar ( Fig. 12I ), a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish cream, butyrous, smooth and semi-glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 3.0–5.7 × 7.0–15.7 μm ( Fig. 12J ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose (variable), sucrose (variable), maltose, cellobiose, trehalose, lactose (delayed and weak), raffinose, inulin (variable), soluble starch (variable), Dxylose (variable), L-arabinose (variable), ethanol (variable), glycerol, ribitol (variable), D-mannitol (variable), D-glucitol (variable), DL-lactate (variable) and succinate (variable) are assimilated as sole carbon sources. Galactose, melibiose, melezitose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), L-lysine (weak) and ethylamine hydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26– 27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Kon. arboricola differs from its closely related species Kon. foliicola in its inability to assimilate melezitose, Darabinose and D-glucosamine and its ability to assimilate maltose, grow in vitamin-free medium and produce starch like compounds ( Table S1.17 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of tree, Sep. 2004, F.-Y. Bai (holotype CGMCC 2.2621 T preserved in a metabolically inactive state, ex-type CBS 15452 = XZ12B5).

Figures

Fig. 12. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Fi. mucilaginum CGMCC 2.3463T; (B) Pha. aurantiaca CGMCC 2.5601T; (C, D) Kon. cylindrica CGMCC 2.3102T; (E, F) Kon. chamaenerii CGMCC 2.2652T; (G, H) Kon. foliicola CGMCC 2.3100T; (I, J) Kon. arboricola CGMCC 2.2621T; (K, L) Kon. lulangica CGMCC 2.2762T; (M, N) Kon. daliangziensis CGMCC 2.5610T, (O) Kon. ribitophobia CGMCC 2.4441T; (P) Kon. myxariophila CBS 8379T. Bars = 10 μm.