Genolevuria pseudoamylolytica Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 83-84

Genolevuria pseudoamylolytica Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828740. Fig. 7F .

Etymology: the specific epithet pseudoamylolytica refers to the similar colony morphology to that of Genolevuria amylolytica .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and subglobosal, 2.9– 5.2 × 3.3– 7.7 μm and single, budding is polar ( Fig. 7F ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is cream, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin (weak), soluble starch, D-xylose (weak), Larabinose, D-arabinose, D-ribose, L-rhamnose (weak), Dglucosamine, N-Acetyl-D-glucosamine, D-mannitol (weak), Dglucitol (weak), Methyl-α- D-glucoside and salicin are assimilated as sole carbon sources. Methanol, ethanol, glycerol, erythritol, ribitol, galactitol, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, G. pseudoamylolytica differs from the two closely related species, G. amylolytica and G. tibetensis , in its inability to assimilate ribitol and succinate and the ability to assimilate L-sorbose and potassium nitrate ( Table S1.2 ).

Typus: China, Daliangzi river national forest park , Heilongjiang province, obtained from a leaf of an unidentified plant, Aug. 2014, Q.-M. Wang (holotype CGMCC 2.5809 T preserved in a metabolically inactive state, ex-type CBS 13955 = HLJ1B6).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.