Saitozyma pseudoflava Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 87-89

Saitozyma pseudoflava Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828758. Fig. 8A .

Etymology: the specific epithet pseudoflava refers to the similar colony morphology to that of Saitozyma flava .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ovoid, 3.2– 4.3 × 5.2–6.8 μm and single, budding is polar ( Fig. 8A ), a sediment is formed. After 1 mo at 17 °C, a pellicle and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, melibiose (weak), raffinose, melezitose, inulin (weak), Dxylose, L-arabinose, D-arabinose (weak), D-ribose, L-rhamnose (delayed and weak), D-glucosamine (delayed and weak), N- Acetyl-D-glucosamine (delayed and weak), ribitol (delayed and weak), galactitol (delayed and weak), D-mannitol (delayed and weak), D-glucitol, Methyl-α- D-glucoside, salicin (delayed and weak), D-gluconate (delayed and weak) and myo-inositol are assimilated as sole carbon sources. L-sorbose, trehalose, lactose, soluble starch, methanol, ethanol, glycerol, erythritol, DL-lactate, succinate, citrate and hexadecane are not assimilated. Ammonium sulfate, L-lysine and ethylamine hydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sa. pseudoflava differs from its closely related species Sa. paraflava and Sa. flava in its inability to assimilate cellobiose, trehalose, soluble starch, DL-lactate, succinate and citrate ( Table S1.8 ).

Typus: China, Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5811 T preserved in a metabolically inactive state, ex-type CBS 15576 = XZ200A1).

Figures

Fig. 8. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A) Sa. pseudoflava CGMCC 2.5811T; (B) Di. milinica CGMCC 2.5628T; (C, D) Di. heilongjiangensis CGMCC 2.5674T; (E, F) Di. ovata CGMCC 2.3625T; (G, H) Di. maotaiensis CGMCC 2.4537T; (I) Di. kandeliae CGMCC 2.5658T; (J) Bu. phyllostachydis CGMCC 2.5812T; (K, L) Bu. cremeum CGMCC 2.4427T; (M, N) Bu. pseudopanici CGMCC 2.4024T; (O, P) Bu. phyllophilum CGMCC 2.3320T. Bars = 10 μm.