Sporobolomyces reniformis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 121

Sporobolomyces reniformis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828836. Fig. 16I, J .

Etymology: the specific epithet reniformis refers to the reniform ballistoconidia.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal to ovoid, 3.8– 5.7 × 5.8–10.7 μm and single, budding is polar ( Fig. 16I ), a sediment is formed. On YM agar, after 1 mo at 17 °C, the streak culture is orange red, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or reniform, 2.8– 3.8 × 7.5– 10.0 μm ( Fig. 16J ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, L-sorbose, sucrose, maltose, trehalose, raffinose, ethanol (delayed and weak) and DL-lactate are assimilated as sole carbon sources. Galactose, cellobiose, lactose, melibiose, melezitose, inulin, soluble starch, D-xylose, Larabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin, D-gluconate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Potassium nitrate, sodium nitrite, L-lysine and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sp. reniformis differs from its closely related species Sp. ellipsoideus in its inability to assimilate melezitose, D-mannitol and D-glucitol ( Table S1.29 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5627 T preserved in a metabolically inactive state, ex-type CBS 15562 = GPS21.2C2).

Figures

Fig. 16. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ros. petaloides CGMCC 2.3446T; (C, D) Ros. cystopteridis CGMCC 2.2615T; (E, F) Ros. motuoensis CGMCC 2.5819T; (G, H) Sp. cellobiolyticus CGMCC 2.5675T; (I, J) Sp. reniformis CGMCC 2.5627T; (K, L) Sp. ellipsoideus CGMCC 2.5619T; (M, N) Sp. primogenomicus IAM13481T; (O, P) Rh. platycladi CGMCC 2.3118T. Bars = 10 μm.