Sporobolomyces cellobiolyticus Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 121

Sporobolomyces cellobiolyticus Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828835. Fig. 16G, H .

Etymology: the specific epithet cellobiolyticus refers to the physiological character of assimilating cellobiose.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid, ellipsoidal and cylindrical, 2.6– 4.8 × 5.6– 12.0 μm and single, budding is polar ( Fig. 16G ), a sediment is formed. On YM agar, after 1 mo at 17 °C, the streak culture is orange, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or reniform, 1.9– 3.2 × 5.1– 7.1 μm ( Fig. 16H ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), L-sorbose (variable), sucrose, maltose, cellobiose (delayed), trehalose, raffinose (delayed), melezitose, inulin, D-ribose (variable), ethanol (variable), glycerol (variable), ribitol (variable), D-mannitol (variable), D-glucitol, Methyl-α- D-glucoside, salicin (variable), DL-lactate (variable) and succinate (variable) are assimilated as sole carbon sources. Lactose, melibiose, soluble starch, D-xylose, L-arabinose, D-arabinose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, galactitol, D-gluconate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (variable), sodium nitrite (variable), L-lysine, ethylamine hydrochloride (variable) and cadaverine dihydrochloride (variable) are assimilated as sole nitrogen sources. Maximum growth temperature is 26 –27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sp. cellobiolyticus differs from its closely related species Sp. jilinensis in its inability to assimilate soluble starch and D-xylose and its ability to assimilate cellobiose and inulin ( Table S1.29 ).

Typus: China, Wuyiling natural reserve , Heilongjiang province, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5675 T preserved in a metabolically inactive state, ex-type CBS 13964 = HLJ33B4).

Figures

Fig. 16. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ros. petaloides CGMCC 2.3446T; (C, D) Ros. cystopteridis CGMCC 2.2615T; (E, F) Ros. motuoensis CGMCC 2.5819T; (G, H) Sp. cellobiolyticus CGMCC 2.5675T; (I, J) Sp. reniformis CGMCC 2.5627T; (K, L) Sp. ellipsoideus CGMCC 2.5619T; (M, N) Sp. primogenomicus IAM13481T; (O, P) Rh. platycladi CGMCC 2.3118T. Bars = 10 μm.