Sporobolomyces ellipsoideus Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 121-123

Sporobolomyces ellipsoideus Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828837. Fig. 16K, L .

Etymology: the specific epithet ellipsoideus refers to the ellipsoidal cell morphology.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical and ellipsoidal, 2.1– 2.9 × 3.6–8.8 μm and single, budding is polar ( Fig. 16K ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal, allantoid or reniform, 1.2– 2.5 × 5.0– 7.1 μm ( Fig. 16L ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), L-sorbose (variable), sucrose, maltose, cellobiose (variable), trehalose, lactose (variable), raffinose (weak), melezitose, inulin (variable), soluble starch (variable), D-ribose (variable), L-arabinose (variable), Darabinose (variable), L-rhamnose (variable), D-glucosamine (variable), ethanol (variable), glycerol (variable), ribitol (variable), Dmannitol, D-glucitol, Methyl-α- D-glucoside (variable), DL-lactate (variable), succinate (variable), citrate (variable) and salicin (variable) are assimilated as sole carbon sources. Melibiose, D-xylose, N-Acetyl-D-glucosamine, D-gluconate, methanol, erythritol, galactitol, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, Sodium nitrite (variable), Llysine, ethylamine hydrochloride (variable) and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sp. ellipsoideus differs from its closely related species Sp. reniformis in its ability to assimilate melezitose, Dmannitol and D-glucitol ( Table S1.29 ).

Typus: China, Milin county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2015, Q.-M. Wang (holotype CGMCC 2.5619 T preserved in a metabolically inactive state, ex-type CBS 15590 = GPS21.5C1).

Figures

Fig. 16. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ros. petaloides CGMCC 2.3446T; (C, D) Ros. cystopteridis CGMCC 2.2615T; (E, F) Ros. motuoensis CGMCC 2.5819T; (G, H) Sp. cellobiolyticus CGMCC 2.5675T; (I, J) Sp. reniformis CGMCC 2.5627T; (K, L) Sp. ellipsoideus CGMCC 2.5619T; (M, N) Sp. primogenomicus IAM13481T; (O, P) Rh. platycladi CGMCC 2.3118T. Bars = 10 μm.