Sporobolomyces primogenomicus Q.M. Wang & F.Y. Bai, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 123

Sporobolomyces primogenomicus Q.M. Wang & F.Y. Bai sp. nov. MycoBank MB828838. Fig. 16M, N .

Etymology: the specific epithet primogenomicus refers to the fact that the type strain was the first sequenced genome in the Pucciniomycotina.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.0– 3.8 × 3.0–5.6 μm and single, budding is polar ( Fig. 16M ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is red, butyrous, shiny. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.0 –2.7 × 3.3– 5.8 μm ( Fig. 16N ).

Physiological and biochemical characteristics: Glucose, galactose, L-sorbose, sucrose, maltose, cellobiose, trehalose, raffi- nose, melezitose, soluble starch, D-xylose, L-arabinose, Darabinose, D-ribose, glycerol, ribitol, D-mannitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, succinate and citrate (delayed) are assimilated as sole carbon sources. Lactose, melibiose, inulin, L-rhamnose, erythritol, galactitol and myo-inositol are not assimilated. Potassium nitrate (weak) is assimilated as sole nitrogen sources.

Physiologically, Sp. primogenomicus differs from its closely related species Sp. ruberrimus in its ability to assimilate L-sorbose, soluble starch, D-xylose, L-arabinose, D-arabinose, Dribose, ribitol, D-glucitol, Methyl-α- D-glucoside and DL-lactate ( Table S1.29 ). The data of carbon assimilation were collected from Yamazaki & Komagata (1983).

Typus: Japan, Kanto region, obtained from a willow leaf, 1983, M. Yoshizawa (holotype JCM 8242 T preserved in a metabolically inactive state, ex-type CBS 15935 = IAM13481).

Figures

Fig. 16. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Ros. petaloides CGMCC 2.3446T; (C, D) Ros. cystopteridis CGMCC 2.2615T; (E, F) Ros. motuoensis CGMCC 2.5819T; (G, H) Sp. cellobiolyticus CGMCC 2.5675T; (I, J) Sp. reniformis CGMCC 2.5627T; (K, L) Sp. ellipsoideus CGMCC 2.5619T; (M, N) Sp. primogenomicus IAM13481T; (O, P) Rh. platycladi CGMCC 2.3118T. Bars = 10 μm.