Phyllozyma aceris Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 113

Phyllozyma aceris Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828813. Fig. 15A, B .

Etymology: the specific epithet aceris refers to Acer , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are cylindrical, 2.6– 3.5 × 5.5–8.9 μm and single, budding is polar ( Fig. 15A ), a sediment is present. After 1 mo at 17 °C, a pellicle and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pink-orange, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid, falcate or cylindrical, 2.5– 3.7 × 10.0– 13.3 μm ( Fig. 15B ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, trehalose, raffinose, inulin (delayed and weak), ribitol (delayed and weak), D-mannitol, Dglucitol (weak) and succinate (weak) are assimilated as sole carbon sources. Galactose, L-sorbose, maltose, cellobiose, lactose, melibiose, melezitose, soluble starch, D-xylose, Larabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, ethanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 20 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Phy. aceris differs from its closely related species Phy. coprosmicola in its inability to assimilate glycerol, Dgluconate, DL-lactate and sodium nitrite ( Table S1.22 ).

Typus: China, Bomi county, Tibet, obtained from a leaf of Acer caudatum, Sep. 2004 , F.-Y. Bai (holotype CGMCC 2. 2662 T preserved in a metabolically inactive state, ex-type CBS 15773 = XZ17B1).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.