Cystobasidium terricola Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 116

Cystobasidium terricola Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828823. Fig. 15N .

Etymology: the specific epithet terricola refers to the origin of the substrate of the type strain, soil.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.1– 4.2 × 2.8– 5.7 μm and single, budding is polar ( Fig. 15N ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pink-red, mucoid, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (variable), L-sorbose (variable), sucrose, cellobiose, trehalose, lactose, raffinose (variable), melezitose, D-xylose, L-arabinose, D-arabinose (delayed and weak), D-ribose, ethanol, glycerol, ribitol, D-mannitol, D-glucitol, salicin, DL-lactate (delayed and weak), succinate and citrate (variable) are assimilated as sole carbon sources. Maltose, melibiose, inulin, soluble starch, L-rhamnose, D-glucosamine, methanol, erythritol, galactitol, Methyl-α- D-glucoside, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (delayed and weak), sodium nitrite (delayed and weak), L-lysine (delayed and weak), ethylamine hydrochloride (delayed and weak) and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 35 °C. Growth in vitamin-free medium is weak. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Cy. terricola and its three closely related species, Cy. raffinophilum , Cy. minutum and Cy. fimetarium , are distinguishable by the assimilation of L-sorbose, galactose, lactose, raffinose, melezitose, galactitol, D-glucitol, salicin, DLlactate and potassium nitrate ( Table S1.26 ).

Typus: China, Yecheng county, Xinjiang province, obtained from soil, Jul. 2007, Q.-M. Wang (holotype CGMCC 2.3823 T preserved in a metabolically inactive state, ex-type CBS 15650 = 140.23).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.