Pupinidius melinostoma melinostoma ( Moellendorff, 1901 )

Wu, Min & Zheng, Wei, 2009, A review of Chinese Pupinidius Moellendorff (Gastropoda, Stylommatophora: Enidae), with the description of a new species, Zootaxa 2053, pp. 1-31 : 8-11

5. Pupinidius melinostoma melinostoma ( Moellendorff, 1901)

Figs 1 , 3 C–D, 8C, 11

Material examined. HBUMM-05480 and HBUMM-05481 (56 fmss, 33 young): 943 m a.s.l., 32°56΄33.5΄ N, 104°40΄33.2΄ E, bank of Bailongjiang River, Wenxian , Gansu, 2006-IX-27, coll. M.Wu, J.Liu, W.Zheng, L.Gao; HBUMM-05577 (13 fmss. All measured. Specimen-1 for dissection): 789 m a.s.l., 32°51΄41.6΄ N, 104°50΄43.1΄ E, Hendan, Wenxian , Gansu, 2006-IX-29, coll. M.Wu, J.Liu, W.Zheng, L.Gao; HBUMM-05548 (180 fmss, 1 nearly matured): from 829 m a.s.l., 32°51΄44.7΄ N, 104°50΄48.2΄ E to 779 m a.s.l., 32°51΄49.4΄ N, 104°50΄37.7΄ E, Hendan, Wenxian , Gansu, 2006-IX-29, coll. M.Wu, J.Liu, W.Zheng, L.Gao. SMF-41978: Lectotype. Shy-pu am Pui-ho, S. Kangsu, China. Slg. O.v.Moellendorff. SMF-41979: Paratypes. 2 fmss. Shypu am Pui-ho, S. Kangsu, China. Slg. O.v.Moellendorff. SMF-41980: Paratypes. 3 fmss (one apically crashed). Fluss Lum-pu, Pui-ho, Kangsu, China. Slg. O.v.Moellendorff (measurement of types: ewh 1.500–1.575–1.750, wh 6.500–6.625–6.750, h 20.2–20.9–21.7, diam. maj. 10.8–11.8–13.3, aph 9.3–9.9–11.0, apw 7.3–7.6–8.0, h/diam. maj. 1.64–1.78–1.99, h/aph 1.98–2.12–2.19).

Distribution. S Gansu (type locality).

Type locality: Shypu am Puiho, River Lum-pu (S Gansu, China).

Diagnosis: Shell bandless; striate. Aperture with angular tubercle. Most swollen part at penultimate and body whorls. With 6.250–7.000 whorls; height 20.2–24.8, diam. maj. 10.8–13.1. Penis without penial caecum; internally with more than two fine longitudinal pilasters. Papilla developed by fused proximal pilasters forming V-shaped structure present. Epiphallus forming numerous loops. Epiphallic caecum absent.

Re-description of shell. Cylindrical-conic; Shell slightly apex protruded. Most swollen part at penultimate and body whorls. Shell dextral; thin; solid; opaque; glossy; more or less speckled (below apex); not spirally grooved; with 6.250–6.740–7.000 whorls. Growth lines usually not very clear. Whorls not shouldered; more or less flattened. Embryonic shell smooth; polished; with 1.375–1.538–1.750 whorls. Postnuclear whorls smooth. Suture normal, without narrow defined zone on beneath whorl. Last whorl gradually ascending towards aperture; rounded at periphery; without smoothed spiral peripheral depression nor rugate region. Shell 20.9–22.9–24.8 high; 11.3–12.1– 13.1 in diam. maj. Height/width ratio 1.76–1.90–2.07. Aperture in a plane; completely attached to body whorl; truncate-ovate; not adnate; slightly oblique; not armed; distinctly with angular tubercle; 10.0–10.7–12.1 high; 7.2–8.0–8.8 wide. Ratio of shell height to aperture height 2.04–2.15–2.28. Peristome strongly reflexed, without distinct cuff; expanded; sharp. Parietal callus indistinct. Columellar margin slightly reflexed. Columella arched. Outer edge of columellar lip vertical. Umbilicus narrowly open. Apical whorls normally coloured. Shell multicoloured; with dark chestnut background, below apex along growth lines with irregularly distributed thick or thin white stripes, which are sometimes densely spaced. Aperture reddish brown; not brightly banded; spirally without colour band.

Re-description of genitalia. Vas deferens indistinctly swollen in middle portion; entering epiphallus apically with distinct demarcation. Epiphallus rather long; cylindrical; of uniform thickness; forming numerous loops; externally smooth. Epiphallic caecum absent. Flagellum short; tubular; proximally normal; with tip blunt. Penis clavate; without penial caecum; distally enlarged; thin-walled; internally with more than 2 fine longitudinal pilasters. Other structure absent. Penis internally V-shaped pilaster with proximal free end approaching at penial retractor insertion. Papilla developed by fused distal pilasters forming V-shaped structure present. Penial process low sac-like; situated at boundary between epiphallus and penis. Penial verge absent. Penial appendix moderately long; branched off from penis at some distance from atrium; divided into sections including A-1+A-2, A-3 and A-4+A-5. A-1 short; interiorly with evenly arranged transversal folds. A-1 and A-2 fused. A-3 opening into A-2 by a long needle-like papilla. Boundary between A-4 and A-5 indistinct. A-5 short; straight. Penial retractor biramous; attaching to penis distally and to A-2 of penial appendix; with arms of penial retractor arising from diaphragm closed to each other. Additional retractor rather than penial or appendicular absent. Muscular band connecting vagina and epiphallus absent. Atrium short; without retractor. Free oviduct longer than vagina. Vagina very short; not swollen; straight; not lined with loose, spongy tissue; unpigmented. Bursa copulatrix duct rather long; proximally strongly twisted. Bursa copulatrix with stalk; without apical ligament; small; with short neck; poorly defined. Diverticle normally present; longer than reservoir; terminally expanded. Bursa copulatrix and diverticle quite indistinguishable; forked more distally from their base.

Measurement of genitalia. P: 5.1; Ep: 26.3; Fl: 0.8; VD: 4.2; Va: 2.3; FO: 3.3; BCD: 28; BC: 1.6; D: 7.5; A-1+A-2: 3.1; A3: 1.4; A-4+A-5: 12.6 (in HBUMM-05577-specimen 1).

Remarks. The specimens of new collection show the fewer whorl number as 6.25–7 than 7.5 in the original description ( Moellendorff 1901). The fresh specimens are distinctly slenderer than types in shell build. However, the large overlap for the value of height/diam. maj. of the fresh specimens and the types demonstrates they belong to the same subspecies.

Figures

FIGURE 1. Distribution map of Pupinidius spp. Red dot, Pupinidius anocamptus, P. nanpingensis nanpingensis and P. nanpingensis ambigua; red circle, P. porrectus; black dot, P. pupinella altispirus; black circle, P. chrysalis; blue dot, P. obrutschewi contractus and P. pupinella pupinella; blue circle: P. latilabrum; green dot, P. melinostoma, P. nanpingensis nanpingensis and P. wenxian sp. nov.; yellow dot, P. obrutschewi obrutschewi and P. streptaxis; yellow circle, P. porrecta pygmaea. P. pupinidius and P. gregorii have not been mapped here due to the unrecognizable locality names. Although the original description of the type locality of P. chrysalis is not accurate and geographically wrong, mapping of the species is possible here based only on the recognized information therein.

FIGURE 3. A – B, Pupinidius latilabrum (Annandale, 1923). A, NHM-Ellis Coln Acc. No. 2136, original series; B, HBUMM- 04328 - specimen 1. C – D, Pupinidius melinostoma melinostoma (Moellendorff, 1901). C, HBUMM- 05577 - specimen 6; D, Lectotype, SMF- 41978. E – F, Pupinidius nanpingensis ambigua (Moellendorff, 1901). E, Lectotype, SMF- 41996; F, one paratype, SMF- 41997.