Cotesia Cameron 1891

Fagan-Jeffries, Erinn P. & Austin, Andrew D., 2020, Synopsis of the parasitoid wasp genus Cotesia Cameron, 1891 (Hymenoptera: Braconidae: Microgastrinae) in Australia, with the description of seven new species, European Journal of Taxonomy 667 (667), pp. 1-70 : 4-12

Genus Cotesia Cameron 1891

Type species

Cotesia flavipes Cameron, 1891: 185 . For a complete list of synonyms and bibliography, see Fernández- Triana et al. (2020).

Comments

Cotesia is one of the largest genera of Microgastrinae and was previously treated as the glomeratus group of Apanteles sensu lato (s.l.). A detailed description of the genus can be found in Mason (1981), whilst supplementary notes, a discussion of putative relationships and a list of world species can be found in Fernandez-Triana et al. (2020). Members of the genus can be diagnosed by the absence of a fore wing areolet, possessing a short inflexible hypopygium and a short ovipositor, T1 generally parallelsided or broadening posteriorly (occasionally in the Australian fauna with T1 narrowing posteriorly but never to the extent as in Glyptapanteles ) and T2 normally broad and rectangular. Often species have the propodeum with a medial carina, and the propodeum, T1 and T2 coarsely sculptured.

Host information for Australian species of Cotesia

In Australia, species of Cotesia are known to parasitise at least nine different lepidopteran families ( Table 1), with some species appearing highly specialised on individual host species or genera, whilst others (e.g., C. ruficrus ) are recorded from numerous genera within a single family. The host information presented below, and in the following key, are restricted to records from Australia; for extra-limital host records see references listed under the treatment of each species.

Key to the described species of Cotesia in Australia and Papua New Guinea

This key is based on adult females and should be treated with some caution due to the variable nature of some characters in Cotesia , and the limited number of specimens available for some species during the construction of the key. Wherever possible, morphological identification should be supported by COI barcoding or host information.

1. T1 evenly broadening towards posterior margin ( Fig. 2A )................................................................2

– T1 mostly parallel sided or barrel shaped ( Fig. 2C ), in some species broadening on anterior 0.6–0.7 and then curving inwards to meet T2 ( Fig. 2B )...............................................................................10

2. Scutellar disk with large, strong circular punctures ( Fig. 3A ).......................................................3 – Scutellar disk smooth or with small punctures associated with setae ( Fig. 3B ).............................4

3. T3 with only a single row of setae, often in posterior half ( Fig. 4A ) often row of setae is irregular or undulating [gregarious parasitoid of noctuid species]...................... C. ruficrus ( Haliday, 1834)

– T3 with multiple rows of setae, particularly visible at lateral edges ( Fig. 4B ) [solitary parasitoid of Plutella xylostella ]............................................................................... C. vestalis ( Haliday, 1834)

4. Mesosoma dorsoventrally flattened, in lateral view its length more than 2.0 × its height ( Fig. 5A ) [scutellar disk completely smooth, no small punctures, anteromesoscutum punctures widely spaced (distance between punctures sometimes greater than diameter of punctures)].................5

– Mesosoma not dorsoventrally flattened, in lateral view its length less than 2.0 × its height ( Fig. 5B ) [scutellar disk with at least some micropunctures associated with setae]..........................6

5. Face projection between base of antennae slightly rounded, with smooth margin ( Fig. 6C )......... ................................................................................................................. C. flavipes Cameron 1981 *

– Face projection between base of antennae less rounded, with margin appearing almost tri-lobed under high magnification ( Fig. 6 A–B)....................................................... C. nonagriae ( Olliff, 1893) *

6. Hind coxa and metasoma significantly paler than mesosoma, dorsal metasoma light brown to yellow ( Fig. 28 A–B) [parasitoid of the native butterfly Ogyris zosine Hewitson, 1853 ].................................................................................................. C. rufiventris ( Bingham, 1906)

– Hind coxa and metasoma as dark or only slightly paler than mesosoma. Dorsal metasoma dark....7

7. Ocelli small, OOL/posterior ocellus diameter ratio> 2.5 ( Fig. 7A ) [dorsal head smooth and polished with sparse setae, scutum punctate, propodeum strongly sculptured, T2 mostly smooth with some striate sculpturing].............................................................................. C. ocellata sp. nov.

– Ocelli normal sized, OOL/posterior ocellus diameter <2.4 ( Fig. 7B )............................................8

8. Fore wing vein r shorter than 2RS [anteromesoscutum very strongly punctate reticulate along notauli, much smoother in centre and lateral areas, gregarious parasitoid of Agrotis munda (Walker,1857) ].. ............................................................................................................. C. radiantis (Wilkinson, 1929)

– Fore wing vein r normally longer than, occasionally of similar length, to 2RS [anteromesoscutum punctate to punctate reticulate, slightly stronger along lines of notauli and smoother in centre and lateral areas].......................................................................................................................................9

9. Distal quarter of hind tibia darkened, including in lateral view [scutellum sometimes with small posteromedian band of rugosity, parasitoid of the introduced Pieris rapae ( Linnaeus, 1758) in Australia and therefore associated with agricultural crops and urban gardens]............................... ........................................................................................................ C. rubecula ( Marshall, 1885)

– Hind tibia with only a very small darkened patch, mainly in dorsal view [scutellum without posteromedian band of rugosity; parasitoid of the native Uraba lugens Walker, 1866 ] ................................................................................................ C. urabae Austin & Allen, 1989

10. Fore wing vein 2RS extending past junction with r, creating a ‘stub’ ( Fig. 23A ) [ r shorter than or approximately equal length of 2RS, T1 mostly parallel sided, broadening on anterior 0.6–0.7 and then curving inwards to meet T2; Fig 23B ]............................................ C. philoeampa ( Cameron, 1911)

– Fore wing 2RS not creating a ‘stub’ by extending slightly past junction [vein r equal in length or longer than 2RS]..........................................................................................................................................11

11. Anteromesoscutum relatively smooth, slightly punctate or punctulate, only generally sparse, small punctures ( Fig. 8A )................................................................................................................12

– Anteromesoscutum densely sculptured, strongly and coarsely punctate or punctate reticulate ( Fig. 8B )...........................................................................................................................................14

12. T3 densely setose for all of length............................................................ C. kazak (Telenga, 1949) – T3 only sparsly setose, mostly in posterior one-third...................................................................13

13. Ventral hypopygium deeply emarginate ( Fig. 18C ) [gregarious parasitoid of the introduced Pieris rapae ( Linnaeus, 1758) ]....................................................................... C. glomerata ( Linnaeus, 1758)

– Ventral hypopygium not emarginate [gregarious parasitoid of the native Mnesampela privata Guenée, 1858 on Eucalyptus spp.].......................................................... C. geometricae Austin, 2000

14. T3 pale ( Figs 16A , 25A )................................................................................................................15 – T3 mostly dark or dark with pale lateral areas........................................................................16

15. T1–2 mostly pale, antennae pale [introduced into Papua New Guinea, parasitoid of the introduced Erionota thrax (Linnaeus, 1767) ]..................................................... C. erionotae (Wilkinson, 1928)

– T1–2 mostly dark, antennae dark [ Australia, parasitoid of the native Candalides delospila (Waterhouse, 1903) ]............................................................................................ C. reidarum sp. nov.

16. Scutellum with small posteromedian band of rugosity ( Fig. 9A )........................................................ ................................................................................. C. sp. nr icipe Fernández-Triana & Fiaboe, 2017 – Scutellum without small posteromedian band of rugosity ( Fig. 9B )............................................... 17

17. Scutellar disk with very strong, distinct punctures scattered over whole area [anteromesoscutum, propodeum and T1–2 strongly sculptured, T3 densely setose in posterior two-thirds]................... ............................................................................................................................. C. tjapekki sp. nov.

– Scutellar disk smooth, or with only shallow punctures or dents normally associated with setae.......18

18. T3 with sparse setae only in posterior half [gregarious parasitoid of Delias aganippe (Donovan, 1805) and D. argenthona Fabricius, 1793 ]............................................. C. deliadis ( Bingham, 1906) – T3 with sparse or dense setae over all or at least posterior two-thirds of tergite...............................19

19. T2 ovoid, occasionally semicircle shaped, with very strong, wide crenulate border, T2 often with large punctures on most of tergite ( Fig. 10 A–B)...............................................................20

– T2 semicircle shaped or trapezoid, border with T3 smoothly indented ( Fig. 10D ) or occasionally with fine shallow crenulate border ( Fig. 10C ), T2 often mostly smooth, with shallow and sparse punctures limited to the margins....................................................................................................21

20. Scutoscutellar sulcus with approximately 6–8 large pits [parasitoid of Anthela ocellata (Walker, 1855) ]................................................................................................... C. anthelae (Wilkinson, 1928)

– Scutoscutellar sulcus with approximately 9–11 pits [gregarious parasitoid of Anthela connexa (Walker, 1855) ] ........................................................................................... C. wonboynensis sp. nov.

21. Fore wing vein r subtly curved ( Fig. 11B ) [T1–2 mostly smooth, gregarious parasitoid of Epicoma contristis Hübner, 1823 ] ......................................................................................... C. scripta sp. nov. – Fore wing vein r straight ( Fig. 11A , C–D)......................................................................................22

22. Antennal flagellomere 14 length/width <1.5 (T1–2 mostly smooth)...................... C. lasallei sp. nov. – Antennal flagellomere 14 length/width> 2......................................................................................23

23. Scutellar sulcus generally with eight very strong, large punctures, anteromesoscutum with reticulate rugose sculpturing medially ( Fig. 12A ) [gregarious parasitoid of Catopsilia gorgonphone (Boisduval, 1836) ]................................................................................................................... C. medusae sp. nov.

– Scutellar sulcus generally with more than eight small, irregular punctures, anteromesoscutum punctate medially, with smooth spaces visible between punctures ( Fig. 12B ) [gregarious parasitoid of Opodipthera eucalypti (Scott, 1864) ]........................................ C. australiensis ( Ashmead, 1900)

Figures

Fig. 2. A. Cotesia ocellata sp. nov., T1 (SAMA 32-44404). B. Cotesia reidarum sp. nov., T1 (QM T246703). C. Cotesia deliadis (Bingham, 1906), T1 (paralectotype, NHMUK).

Fig. 3. A. Cotesia ruficrus (Haliday, 1834), scutellar disk (ANIC 32 130230). B. C. rufiventris (Bingham, 1906), scutellar disk (paralectotype NHMUK).

Fig. 4. A. Cotesia ruficrus (Haliday, 1834), T3 (ANIC 32 130230). B. Cotesia vestalis (Haliday, 1834), T3 (WINC).

Fig. 5. A. Cotesia nonagriae (Olliff, 1893), head and mesosoma in lateral view. B. Cotesia ocellata sp. nov., head and mesosoma in lateral view (holotype, SAMA 32-44404).

Fig. 6. A. Cotesia nonagriae (Olliff, 1893), Giru, Australia (WINC). B. C. nonagriae, Bundaberg Australia (WINC). C. C. flavipes Cameron 1981, Indonesia (WINC).

Fig. 28. Cotesia rufiventris (Bingham, 1906), paralectotype, ♀ (NHMUK). A. Mesosoma in dorsal view and T1–3 B. Habitus in dorsal view and fore wing C. Habitus in lateral view.

Fig. 7. A. Cotesia ocellata sp. nov., head in dorsal view (holotype, SAMA 32-44404). B. C. rubecula (Marshall, 1885), head in dorsal view (WINC).

Fig. 23. A–B. Cotesia philoeampa (Cameron, 1911), lectotype, ♀ (NHMUK 3.c.997). A. Fore wing. B. Habitus in dorsal view. C–D. C. rubecula (Marshall, 1885), ♀, Urrbrae (WINC). C. Fore wing D. Habitus in dorsal view.

Fig. 8. A. Cotesia kazak (Telenga, 1949), anteromesoscutum (WINC, Mariginiup specimen). B. C. reidarum sp. nov., anteromesoscutum (holotype, QM T246703).

Fig. 18. A. Cotesia glomerata (Linnaeus, 1758), specimen from South Perth (WINC), habitus in dorsal view. B. C. kazak (Telenga, 1949), specimen from Mariginiup (WINC), habitus in dorsal view. C. C. glomerata, specimen from Victoria (WINC), hypopygium in ventral view, arrow pointing towards emarginated area. D. C. glomerata, fore wing. E. C. kazak, fore wing.

Fig. 16. A. Cotesia erionotae (Wilkinson, 1928), holotype, ♀ (NHMUK 3.c.1037). B. Cotesia geometricae Austin, 2000, paratype (WINC).

Fig. 25. Cotesia reidarum sp. nov., holotype, ♀ (QM T246703). A. Habitus in dorsal view. B. Habitus in lateral view. C. Head in dorsal view. D. Head in anterior view. E. Propodeum. F. Fore wing.

Fig. 9. A. Cotesia sp. nr icipe, medial posterior band of the scutellum (WINC, Cox Scrub). B. C. lasallei sp. nov., medial posterior band of the scutellum (holotype, SAMA 32-44401).

Fig. 10. A. Cotesia anthelae (Wilkinson, 1928), T2 (paratype, NHMUK 3.c.002). B. C. wonboynensis sp. nov., T2 (holotype ANIC 32 130294). C. C. medusae sp. nov., T2 (paratype, QM T246705). D. C. scripta sp. nov., T2 (paratype, MV T22495).

Fig. 11. A. Cotesia australiensis (Ashmead, 1900), fore wing vein r straight (ANIC 32 151532). B. C. scripta sp. nov., fore wing vein r subtly curved (holotype, MV T22495). C. C. lasallei sp. nov., fore wing vein r straight (holotype, SAMA 32-44401). D. C. medusae sp. nov., fore wing vein r straight (paratype QM T246705).

Fig. 12. Scutellar disk, scutellar sulcus and partial anteromesoscutum. A. Cotesia medusae sp. nov. (paratype, QM T246705). B. C. australiensis (Ashmead, 1900) (ANIC 32 151535).