Colletotrichum jiangxiense F. Liu and L. Cai, Persoonia

Yang, Erfu, Tibpromma, Saowaluck, Dai, Dongqin, Promputtha, Itthayakorn, Mortimer, Peter E. & Karunarathna, Samantha C., 2022, Three interesting fungal species associated with the Asian House Gecko in Kunming, China, Phytotaxa 545 (1), pp. 37-56 : 49

Colletotrichum jiangxiense F. Liu and L. Cai, Persoonia 35: 82 (2015) ( Figure 7 )

Index Fungorum number: 809161

Saprobic or pathogenic on dead Asian House Gecko, white, cottony mycelium on the skin. Sexual morph: undetermined. Asexual morph: Mycelium 1.5–3.5 μm wide (x̅ = 2.5 μm, n = 30), granular to smooth, branched, with thick-walled septa. Conidiophores 2.5–5 μm (x̅ = 4 μm, n = 15), straight to slight curved, septate, smooth-walled, narrow in the terminal with a hyaline, cylindrical. Conidiogenous cells 10–20 × 1–3 µm (x̅ = 13 × 2.5 μm, n = 20), cylindric to flask-shaped, tapering at apex to narrow, phialidic conidiogenous locus. Conidia 10–20 × 3–5 μm (x̅ = 14 × 4 μm, n = 20), cylindrical to oblong, hyaline, thick-walled, granular to smooth, around, both ends bluntly rounded, or some see bottom end acute, appressoria not observed.

Substratum: Camellia sinensis ( Liu et al. 2015, Jayawardena et al. 2016 a, Wang et al. 2020); Citrus sinensis ( De Silva et al. 2017) Persea americana ( Fuentes-Aragón et al. 2020) ;

Distribution: China ( Liu et al. 2015, Jayawardena et al. 2016 a, De Silva et al. 2017, Wang et al. 2020); Mexico ( Fuentes-Aragón et al. 2020);

Material examined: China, Yunnan Province, on dead Asian House Gecko, Peter E. Mortimer, F-1 (Herb. KUN-HKAS 122653), living culture, KUMCC 21-0466. Genbank numbers: ITS: OM 670162 , HIS: OM 744410 , CAL: OM 744409 , ACT: OM 744408 , tub2: OM 744407 , GPDH: OM 744411 .

Culture characteristics: Colonies on PDA fast-growing, reach 60 mm in one week, above effuse, aerial mycelium, cottony, light grayish to olivaceous with white margin. Reverse: grayish orange to pale brown, black-brown at the central inoculation, with black pigmented margin, not sporulated, without pigments produced in PDA.

Notes: Colletotrichum jiangxiense was first introduced by Liu et al. (2015) from Jiangxi province, China. Our isolate KUMCC 21-0466 has similar morphologies to members of Colletotrichum , and the BLASTn results of GAPDH, CHS- 1, ACT, tub2, CAL and ITS of our strain indicate 99–100% similarity with C. jiangxiense ZY 12 and C. jiangxiense ( COP 27.905, LF 488). The multi-locus phylogenetic trees also showed our new isolate clusters together with C. jiangxiense ZY 12 with high statistical support values ( ML: 94%, BI: 0.96; Figure 6 ); thus, we report C. jiangxiense KUMCC 21-0466 as a new host record associated with a reptile (Asian House Gecko) in this study.

Figures

FIGURE 6. Phylogram generated from maximum likelihood analysis based on a combined ITS, HIS, CAL, ACT, tub2 and GPDH sequence datasets. Related sequences were taken from Diao et al. (2017) and Chaiwan et al. (2021). The 52 strains are included in the combined gene analyses, 2882 total characters including gaps (ITS: 1–579 bp, HIS: 580–874 bp, CAL: 875–1610 bp, ACT: 1611–1888 bp, tub2: 1889–2602 bp, GPDH: 2603–2882). Tree topology of the ML analysis was similar to the BI. The matrix had distinct alignment patterns, with the final ML optimization likelihood value of -14018.222367 (ln). All free model parameters were estimated using the RAxML model, with 1867 distinct alignment patterns and 15.05% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.227166, C = 0.283924, G = 0.257262, T = 0.216566, with substitution rates AC = 1.035930, AG = 0.298823, AT = 0.248992, CG = 0.225019, CT = 4.115498, GT = 1.000000. The gamma distribution shape parameter alpha = 0.904631 and the Tree- Length = 0.821909. The final average standard deviation of split frequencies at the end of total MCMC generations calculated as 0.009613 in BI analysis. The species determined in this study are indicated in red. Bootstrap values equal to or greater than 70% (ML, left) and Bayesian posterior probabilities (BI, right) equal to or greater than 0.95 are given at the nodes. Hyphens (-) represent support values less than 70% in ML/0.95 in BI.

FIGURE 7. Colletotrichum jiangxiense (KUMCC 21-0466). a, b Colonies on PDA. c Hyphae. d Hyphae stained by cotton blue reagent. e, f Conidophores connected with conidia (f: stained by congo red reagent). g–i Conidia (g: stained by cotton blue reagent). Scale bars: c, d = 20 μm, e–i = 10 μm.