Rhodocollybia utrorensis A. Sattar, M. Kiran & A.N Khalid, 2018

Sattar, Ammara, Kiran, Munazza & Khalid, Abdul Nasir, 2018, Rhodocollybia utrorensis (Agaricales, Basidiomycota): a new species and a first record of the genus Rhodocollybia from Pakistan, Phytotaxa 369 (4), pp. 269-277 : 271-273

Rhodocollybia utrorensis A. Sattar, M. Kiran & A.N Khalid , sp. nov. ( Fig. 2. A–B ; Fig. 3 A–D )

MycoBank No. MB825074

Etymology:—The specific epithet refers to the Utror valley, the region from where the basidiomata were collected.

Diagnosis:—Small to medium slightly rimose dull brown basidiomata with off-white margins, dark brown low umbo; adnate lamellae; clavate to sub-clavate basidia; cylindrical to narrowly clavate cheilocystidia; lageniform to narrowly utrifrom pleurocystidia; smooth, inamyloid ellipsoidal to oblong basidisospores, clamp connections present in all tissues.

Holotype:— PAKISTAN. Khyber Pakhtunkhwa Province, Swat District, Utror valley , 19 August 2016, M. Kiran & A. N. Khalid UTR9 ( LAH35478 ! GenBank no: MH220536 ).

Description: Pileus 3.9–4.8 cm in diam. applanate with a low umbo; surface smooth, glabrous, dull brown (7.5YR, 6/3) in the center becoming off-white towards margin when young, changing to brown (7.5YR, 4/3) with age; margins incurved at first becoming inrolled at maturity, rimose. Lamellae adnate, crowded, wavy edges, off-white when young becoming dull brown (7.5YR, 6/3) towards the edges at maturity; lamellulae in 3 tiers. Stipe 4–11 cm in length, 0.5–1.5 cm in diam, smooth, compressed, hollow, equal to broadening towards base, creamy white with yellowish orange (7.5YR, 8/3) striations. Veil not present. Odor unpleasant. Taste not known.

Basidiospores [60/3/2] (6–) 6.2–9.4 (–10.9) × (3.3–) 3.6–4.7 (4.8) μm, avl × avw = 7.8 × 4.2 μm, Q = 1.8–2.2, avQ = 1.85, ellipsoidal to oblong, hyaline in 5% KOH, smooth, thin-walled, mono-gutulate, with a prominent apiculus, inamyloid. Basidia (22.5–) 23.5–30 (–31) × (5.5–) 6–7.5 (–8.5) μm, hyaline in 5% KOH, thin-walled clavate to sub-clavate, with 2–4 sterigmata; sterigmata 2.2–5.3 μm in length. Pleurocystidia (18) 21–26.5 (–27.5) × (4) 4.5–6 (–6.5) μm, hyaline in 5% KOH, lageniform to narrowly utrifrom. Cheilocystidia (22–) 23–26.5 (–32.5) × (3.5–) 3.9–5 (–6) μm, hyaline in 5% KOH, cylindrical to narrowly clavate. Stiptipellis an intricate trichoderm, hyphae 5.4–16.6 μm thick, hyaline in 5% KOH, arranged in parallel. Pileipellis an intricate trichoderm; hyphae 5.4–10.7 μm thick, hyaline in 5% KOH, interwoven, irregular. Clamp connections present in all tissues.

Additional material examined:— PAKISTAN. Khyber Pakhtunkhwa Province, Upper Dir District, Kalkot, 2044m a.s.l. on the forest floor, gregarious, 20 August 2016, M. Kiran & A. N. Khalid KK93 (LAH35479! GenBank no: MH220537).

Figures

FIGURE 2. Rhodocollybia utrorensis sp. nov. basidiomata (dry); A. LAH35478 (Holotype); B. LAH35479; Scale Bars A= 2.2 cm, B= 1.1 cm Field Photography by Abdul Nasir Khalid.

FIGURE 3. Rhodocollybia utrorensis (LAH35478! Holotype) A. Basidiospores, B. Basidia, C. Cheilocystidia, D. Pleurocystidia; Scale Bars A= 3.5 μm, B= 6.9 μm, C= 5.5 μm, D= 5.9 μm, Drawings by Ammara Sattar.