Isospora brasilsatoae Oliveira and Berto, 2019

Oliveira, Mariana S., Oliveira, Jhon Lennon G., Rodrigues, Mariana B., Silva-Carvalho, Lidiane Maria, Andrade, Lucas De Assis S., Cardozo, Sergian V., Ferreira, Ildemar & Berto, Bruno Pereira, 2019, Isospora brasilsatoae n. sp. (Apicomplexa: Eimeriidae) from thrushes Turdus spp. (Passeriformes: Turdidae) from Brazil, Zootaxa 4555 (3), pp. 433-440 : 434-435

Isospora brasilsatoae Oliveira and Berto n. sp.

Type host: Yellow-legged thrush Turdus flavipes (Vieillot, 1818) (Aves: Passeriformes : Turdidae ).

Other hosts: Pale-breasted thrush Turdus leucomelas Vieillot, 1818 (Aves: Passeriformes : Turdidae ); creamybellied thrush Turdus amaurochalinus Cabanis, 1850 (Aves: Passeriformes : Turdidae ).

Type locality: Parque Nacional do Itatiaia (22°26'57"S, 44°36'25"W), Southeastern Brazil .

Other localities: Cacaria (22°42'51"S, 43°50'38"W) and Guapimirim (22°31'10"S, 43°00'36"W), also from Southeastern Brazil.

Type-material: Photosyntypes, line drawing and oocysts recovered from T. flavipes in 2.5% K 2 Cr 2 O 7 solution ( Williams et al. 2010) are deposited at the Museu de Zoologia at the Universidade Federal Rural do Rio de Janeiro, Brazil, under accession number MZURPTZ2018007. Photomicrographs are also deposited and available (http:// r1.ufrrj.br/labicoc/colecao.html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number P-90/2018. Photographs of the type-host specimen (symbiotype) are deposited in the same collection.

ZooBank registration: To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature ( ICZN, 2012), details of the new species have been submitted to ZooBank. The Life Science Identifier (LSID) for Isospora brasilsatoae is urn:lsid:zoobank.org:act:6C3FEE82-FC9C-4A58-8C17-1713532F03F3 .

Site of infection: Unknown.

Prevalence: Four of 10 (40%).

Sporulation: Exogenous. All oocysts were passed in the feces unsporulated and were fully sporulated by day 7 in K 2 Cr 2 O 7 solution at room temperature (20–25°C).

Etymology: The specific name is derived from the family name of a Brazilian parasitologist Dr Marília de Carvalho Brasil Sato, given in her honor for her contribution to the study of taxonomy and ecology of parasites.

Description ( Figs 1 ; 2 A–C).

Oocyst (n = 15) ovoidal to pyriform, 25-30 × 19-23 (26.8 × 21.1); length/width (L/W) ratio 1.2-1.4 (1.27). Wall bi-layered, delicate, 1.4-1.8 (1.6) thick, outer layer smooth, c. 2/3 of total thickness. Micropyle present, without micropyle cap, wrinkles or invagination of the in inner layer perceptible. Oocyst residuum absent, but 1 to 3 rounded polar granules are present. Sporocyst elongate ovoidal with tapered anterior end (where it is Stieda/sub- Stieda complex), 17-19 × 10-11 (18.1 × 10.5); L/W ratio 1.6-1.9 (1.72). Stieda body present, knob-like, 2.0 high × 2.0 wide. Sub-Stieda body present, trapezoidal, 1.5 high × 3.5 wide. Para-Stieda body absent. Sporocyst residuum present, as a distinctly sub-spherical body consisting of numerous small granules that appear to be membranebounded, 6–9 (7.5). Sporozoites vermiform, with anterior and posterior refractile bodies and striations.

Remarks: Isospora brasilsatoae is different from other coccidian species of Turdidae of the New World on several aspects ( Table 1). It is the only one to have a pyriform shape of the oocysts. It has a micropyle, which is a characteristic present only in the oocysts of Isospora albicollis Lainson and Shaw, 1989 , Isospora sabiai Pinho, Rodrigues, Silva, Lopes, Oliveira, Ferreira, Cardozo, Luz, Ferreira, Lopes and Berto, 2017 and Isospora machadoae Pinho, Silva, Rodrigues, Lopes, Oliveira, Luz, Ferreira, Lopes and Berto, 2018 ( Lainson & Shaw 1989; Pinho et al. 2017a; 2017b; 2018). From these three, but also from the others, I. brasilsatoae can be easily differentiated by having up to three rounded polar granules, sporocysts elongate ovoidal (L/W ratio> 1.6), sub- Stieda body trapezoidal, and compact residuum.

Figures

FIGURE 1. Line drawing of the sporulated oocyst of Isospora brasilsatoae n. sp, a new coccidium species recovered from the yellow-legged thrush Turdus flavipes, the pale-breasted thrush Turdus leucomelas and the creamy-bellied thrush Turdus amaurochalinus. Scale-bar: 10µm.

FIGURE 2. Photomicrographs (A-C) of sporulated oocysts of Isospora brasilsatoae n. sp, a new coccidium species recovered from the yellow-legged thrush Turdus flavipes (A), the pale-breasted thrush Turdus leucomelas (B) and the creamy-bellied thrush Turdus amaurochalinus (C). Note the micropyle (m), polar granule (pg), Stieda body (sb), sub-Stieda body (ssb), sporocyst residuum (sr), striations (str) and the refractile body (rb). Scale-bar: 10µm.