Heteropriapulus heterotylus

Acosta, Aline Angelina, Franceschini, Lidiane, Zago, Aline Cristina, Scholz, Tomáš & Silva, Reinaldo José Da, 2017, Six new species of Heteropriapulus (Monogenea: Dactylogyridae) from South American fishes with an amended diagnosis to the genus, Zootaxa 4290 (3), pp. 459-482 : 465-466

Heteropriapulus heterotylus

( Fig. 2 )

Syn. Heterotylus heterotylus Jogunoori, Kritsky & Venkatanarasaiah, 2004

Material studied. HOlOtype ( USNPC 93651 ) and 4 paratypeS ( BMNH 2003.9.4.4-12, 19, 23, 24 and 26); 20 SpecimenS frOm Pterygoplichthys ambrosettii frOm the Aguapeí RiVer : 8 VOucherS in CHIOC (38817–38820a, b; 38821a, b; 38822), 8 VOucherS in CHIBB (244–251L) and 4 VOucherS in IPCAS (M-652).

Type host. Hypostomus Sp. ( SilurifOrmeS : LOricariidae ).

Type locality. Aquaria in Hyderabad and Secunderabad, India (fiSh hOStS Were impOrted Via the aquarium trade).

Additional hosts. Hypostomus plecostomus , Pterygoplichthys ambrosettii (neW hOSt recOrd), P. disjunctivus (Weber) and P. pardalis (CaStelnau) (all SilurifOrmeS : LOricariidae ).

Site of infection. GillS.

Distribution. India (intrOduced), Brazil (Aguapeí RiVer, municipality Of CaStilhO, Paraná RiVer baSin, SãO PaulO State, Brazil, 21°3'36.20"S, 51°45'38.58"W—neW geOgraphical recOrd), China (intrOduced), MeXicO (intrOduced).

Representative DNA sequence. 1,575 bp lOng Sequence Of the D1–D3 regiOn Of the lsr DNA Of One Specimen frOm P. ambrosettii (GenBank AcceSSiOn NO. MF 116370 ).

Remarks. In additiOn tO the Original deScriptiOn, thiS SpecieS WaS alSO fOund On H. plecostomus intrOduced tO China ( Li & Huang, 2012), P. disjunctivus intrOduced tO Japan ( Nitta & NagaSaWa, 2013), and inVaSiVe P. pardalis and P. disjunctivus in MeXicO (ROdríguez-SantiagO et al., 2015, 2016). SpecimenS fOund On P. ambrosettii frOm Brazil Were identified aS H. heterotylus and their identificatiOn WaS cOnfirmed by cOmpariSOn With the hOlOtype and paratypeS Of H. heterotylus . NeVertheleSS, Slight difference WaS fOund betWeen SOme Of the meaSurementS Of the neW material frOm Brazil and thOSe in the Original deScriptiOn, eSpecially in greater Size Of the preSent SpecimenS (See Table 2). The cirruS Of the Brazilian SpecimenS preSentS 1½ cOunterclOckWiSe Spiral ringS neXt tO the rOunded baSe, WhereaS the cirruS Of SpecimenS frOm type hOSt preSentS, accOrding tO JOgunOOri et al. (2004), an incOnSpicuOuS prOXimal bend. In the Original deScriptiOn, JOgunOOri et al. (2004) State that the acceSSOry piece iS lOng, Variable and apparently With fOur SubunitS, but Only three SubunitS Were ObSerVed in the SpecimenS frOm Brazil ( Fig. 2 C). In the preSent Study, firSt mOlecular data On H. heterotylus are prOVided ( Fig. 10 ), a neW fiSh hOSt iS added and the diStributiOn area iS enlarged including the mOSt prObably Original diStributiOn area Of the SpecieS.

Figures

FIGURE 2. (A – H) Sclerotized structures of Heteropriapulus heterotylus (Jogunoori, Kritsky & Venkatanarasaiah, 2004) from Pterygoplichthys ambrosettii (Holmberg) from the Upper Paraná River, Brazil. A, ventral anchor. B, dorsal anchor. C, male copulatory complex. D, vagina. E, hook. F, egg. G, dorsal bar. H, ventral bar.

FIGURE 10. Maximum likelihood topology based on partial 28 S rDNA sequences of selected monogenean species. Newly sequenced species from this study are in dark blue. Posterior probabilities followed by bootstrap support values are given above the branches. Pseudorhabdosynochus epinepheli, P. lantauensis and Pseudomurraytrema sp. were used as outgroups. Branch length scale bar indicates number of substitution per site.