Conlarium nanningense L.Xie, Y.L.Chen & B.Liu

Xie, Ling, Chen, Yan-Lu, Long, Yan-Yan, Zhang, Yan, Liao, Shi-Tong, Liu, Bin, Qin, Li-Ping, Nong, Qian & Zhang, Wen-Long, 2019, Three new species of Conlarium from sugarcane rhizosphere in southern China, MycoKeys 56, pp. 1-11 : 3

Conlarium nanningense L.Xie, Y.L.Chen & B.Liu sp. nov. Figure 1

Etymology.

The species is named for Nanning City, the type locality.

Type.

CHINA. Guangxi: Nanning City , Datang Town . 22°23'25"N, 108°23'12"E, 144 m alt., in sugarcane rhizosphere, 11 Feb. 2011, L. Xie, M1 ( HMAS 247075 holotype) deposited in Microbiology Research Institute, Guangxi Academy of Agricultural Science.

Description.

Colony reached 22 mm diameter on PDA medium after 2 weeks, grey-white to grey-brown, nearly circular, flat growth, less aerial hyphae. Hyphae grey-brown, verruculose, septate. Conidiophores 1-15 × 1-5 μm (6 ± 3 × 4 ± 1 µm, n = 54), stubby, unbranched, septate or aseptate, straight or flexuous, hyaline, becoming brown with age. Conidiogenous cells determinate, doliiform, cylindrical, 4-13 × 5-10 µm (6 ± 2 × 7 ± 2 µm, n = 22). Conidia brown, muriform, irregularly globose or subglobose, smooth, constricted at the septa, 0-1 transversely septa, 0-4 longitudinal septa, 11-21 × 9-21 µm (15 ± 3 × 13 ± 3 µm, n = 50). Chlamydospores subglobose or irregular, 4-12 µm (7 ± 2 µm, n = 67). Sexual morph: undetermined.

Habitat and distribution.

In sugarcane rhizosphere soil of southern China.

Other specimens examined.

CHINA. Guangxi: Nanning City , Datang Town . 22°29'54.51"N, 108°24'3.06"E, 102 m alt., in sugarcane rhizosphere, 11 Feb. 2011, L. Xie, M8 ( HMAS 247985 ).

Notes.

Conlarium nanningense is similar to the asexual morph of C. aquaticum , C. duplumascospora , and C. thailandense . They all have monoblastic, holoblastic conidiogenous cells and mostly irregular, brown, clathrate, muriform conidia ( Liu et al. 2012). However, C. nanningense can be easily distinguished from C. aquaticum , C. duplumascospora , and C. thailandense by the number of conidial septa (2-4-transversely septate, 1-3-longitudinally septate in C. duplumascospora ; 6-12-transverse septa, 4-10-longitudinal septa in C. aquaticum ; 4-8-transverse septa, 4-6-longitudinal septa in C. thailandense vs 0-1 transversely septa, 0-4 longitudinal septa in C. nanningense ) and conidial size (15.5-35 × 11-26.5 μm in C. duplumascosporum , 45-70 × 20-57 μm in C. aquaticum , 25-45 × 17-33 μm in C. thailandense and 11-21 × 9-21 μm in C. nanningense ) ( Liu et al 2012; Zhang et al. 2017; Phookamsak et al. 2019). Phylogenetic reconstructions based on SSU+ITS+LSU+RBP2 sequences show authentic C. nanningense is sister to C. duplumascospora . A comparison of ITS pairwise indicates that C. nanningense differs from C. aquaticum , C. duplumascospora , and C. thailandense in 21 bp, 12 bp, and 18 bp, respectively. Thus, following the guidelines of Jeewon and Hyde (2016), this is a new species.