Fusarium liriopes Y. K. He, Yong Wang

He, Yu-Ke, Jayawardena, Ruvishika S., Kakumyan, Pattana & Wang, Yong, 2023, Taxonomy and phylogeny of Fusarium houttuyniae and F. liriopes spp. nov. (Hypocreales, Nectriaceae) from China, Phytotaxa 603 (3), pp. 260-270 : 266-267

Fusarium liriopes Y. K. He, Yong Wang bis, sp. nov. Fig. 3 .

Mycobank: MB846237; FOF number: 14110

Etymology:— liriopes refers to the host genus Liriope , which the holotype was isolated.

Holotype:— CHINA, Guangxi Province, Nanning City, Guang Xi Medicinal Botanical Garden , 108°19’ E, 22°51’ N, leaf of Liriopes platyphylla , November 2017, collected by Qian Zhang (Collection number: HGUP 10010 , culture number: GUCC 21005)

Additional specimens examined:— CHINA, Guangxi Province, Nanning City, Guang Xi Medicinal Botanical Garden, 108°19’ E, 22°51’ N, leaf of Liriopes platyphylla , November 2017, collected by Qian Zhang (Collection number: HGUP 10010, culture number: GUCC 21009)

Description: Colonies on PDA media with an average radial growth rate of 2.0‒ 2.5 mm /d at 24℃. Colony surface is pale yellow to pinky purple, floccose with a small amount of aerial mycelium which produces an almost pale pink exudate. Colony margins irregular, serrate or filiform, odour absent. Reverse pale yellow to earth yellow. Mycelium is composed of septate, branched, hyphae hyaline, with plentiful chlamydospores, aerial mycelium is sparse with abundant sporulation on the medium surface. Conidiophores unbranched or sparingly branched, aerial phialides subulate to subcylindrical, straight, thin- and smooth-walled. Microconidia hyaline, ellipsoidal to falcate, smooth- and thin-walled, 0‒1 septate; 0-septate conidia: 3‒12 × 2‒4 (av. 7.35 ± 1.9 × 3.01 ± 0.47) µm; 1-septate conidia: 9‒21 × 3‒6 (av. 17.42 ± 2.45 × 4.75 ± 0.66) µm. Macroconidia formed on aerial mycelium, falcate, curved dorsiventrally with almost parallel sides tapering slightly towards both ends, with a blunt to papillate, curved apical cell and a blunt to foot-like basal cell, 1‒3-septate, hyaline, smooth- and thin-walled; 1-septate conidia: 11‒15 × 2‒3 (av. 13.6 ± 0.88 × 2.54 ± 0.25) µm; 2-septate conidia: 14‒22 × 2‒4 (av. 19.1 ± 1.68 × 2.95 ± 0.41) µm; 3-septate conidia: 18‒28 × 3‒5 (av. 25 ± 1.88 × 4 ± 0.39) µm. Chlamydospores globose to sub globose, formed terminally or in the middle, 4‒10 (av. 6.81 ± 1.59) µm diam.

Notes: Phylogenetically, F. liriopes (GUCC 21005) clustered with F. nirenbergiae (CBS 129.24) and F. houttuyniae (GUCC 21004). The comparison of F. liriopes and F. nirenbergiae show that there are 7/606 character differences in cmdA, 2/876 characters in rpb2, 8/621 characters in tef1, and 3/534 characters in tub2. Morphologically, F. liriopes is distinct from F. nirenbergiae based on macroconidial septation ( F. liriopes : 0‒3-septate vs. 0‒5-septate: F. nirenbergiae ( Zhao et al. 2020)) and the size of macroconidia ( F. liriopes : 11‒28 × 2‒5 µm vs. 7‒42 × 3.7‒4.3 µm: F. nirenbergiae ( Zhao et al. 2020)) . The morphological characteristic comparison between F. liriopes and F. houttuyniae is mentioned in notes of F. houttuyniae . Fusarium houttuyniae differs from F. liriopes by having bigger conidia ( F. houttuyniae : 1-septate conidia: 12‒20 × 2‒5 µm; 2-septate conidia: 15‒22 × 2‒4 µm; 3-septate conidia: 28‒45 × 3‒6 µm. vs. F. liriopes : 1-septate conidia: 11‒15 × 2‒3 µm; 2-septate conidia: 14‒22 × 2‒4 µm; 3-septate conidia: 18‒28 × 3‒5 µm.).

Figures

FIGURE 3. Fusarium liriopes (Specimen code: HGUP 10008) a, b. Colony on PDA; a. Surface of colony on PDA after 21 days at 24 °C under continuous white light; b. Reverse of colony on PDA; c, d. Mycelium on PDA; e, h. Conidiophores and phialides on aerial mycelium; i, j. Chlamydospores; k, r Aerial conidia (microconidia); s, t Sporodochia conidia (macroconidia). Scale bars: a, b = 10 mm; c, d = 1 mm; e, g, k, r = 5 µm; h, j, s, t = 15 µm.