Diaporthe sojae Lehman, Ann. Mo. bot. Gdn 10: 128 (1923)

Yang, Qin, Tang, Jie & Zhou, Guo Y., 2021, Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species, MycoKeys 84, pp. 15-33 : 15

Diaporthe sojae Lehman, Ann. Mo. bot. Gdn 10: 128 (1923)

Figure 5

Description.

Sexual morph: perithecia on pine needles in culture, black, globose, 250-500 μm in diam., densely clustered in groups, deeply immersed with elongated, tapering perithecial necks protruding through substrata, 525-800 μm. Asci unitunicate, 8-spored, sessile, elongate to clavate, (35-)37-42(-44.5) × (8-)10-11.5 μm (n = 30). Ascospores hyaline, two-celled, often 4-guttulate, with larger guttules at centre and smaller one at ends, elongated to elliptical, slightly or not constricted at septum, (9-) 9.5-11.5 × 2.7-4 μm (n = 30). Asexual morph not observed.

Culture characters.

Culture incubated on PNA at 25 °C, originally white, fluffy aerial mycelium, reverse yellowish pigmentation developing in centre, later becoming dark brown, with yellowish-cream drops exuding from the perithecia after 15 days.

Specimens examined.

China. Hunan Province: Zhuzhou City , on leaves of Camellia oleifera , 27°2'41"N, 113°19'17"E, 14 Aug. 2020, Q. Yang (USUFT 022; living cultures: HNZZ022, HNZZ008 and HNZZ010) .

Notes.

Diaporthe sojae was first reported on pods and stems of soybean, and subsequently reported on a wide range of hosts (Dissanayake et al. 2015; Udayanga et al. 2015; Guo et al. 2020). It was also reported on some fruit trees in China, such as Vitis spp. (Dissanayake et al. 2015) and Citrus spp. ( Huang et al. 2015). In the present, three isolates (HNZZ008, HNZZ010 and HNZZ022) are closely related to D. sojae in the combined phylogenetic tree (Fig. 1 ). The differences of nucleotides in the concatenated alignment (1/460 in ITS, 3/458 in cal, 1/320 in his3 and 3/433 in tub2) are minor. Compared with the description of the ex-type isolate FAU635, the isolate has wider asci (10-11.5 μm vs. 7-9 μm) ( Udayanga et al. 2015). We therefore identify the isolates as belonging to D. sojae .

Figures

Figure 1. Phylogram of Diaporthe resulting from a maximum likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Numbers above the branches indicate ML bootstraps (left, ML BS ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.75). The tree is rooted with Diaporthella corylina. Isolates in current study are in blue. " - " indicates ML BS <50 % or BI PP <0.75.

Figure 5. Diaporthe sojae (HNZZ 022) A Culture on PNA B ascomata C-E asci and ascospores. Scale bars: 500 μm (B); 10 μm (C-E).